Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,672,863	A→G	L96P (CTG→CCG)	mdtI ←	multidrug efflux system transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,672,863	0	A	G	100.0%	222.5 / NA	62	L96P (CTG→CCG)	mdtI	multidrug efflux system transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/30);  total (32/30)

GCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAAA  >  NC_000913/1672728‑1672995                                                                                                                                        |                                                                                                                                     gCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGc                                                                                                                                   <  2:449070/139‑1 (MQ=255)        cTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCa                                                                                                                            <  1:819359/139‑1 (MQ=255)             aCCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                       >  2:629741/1‑139 (MQ=255)                   tttCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGAt                                                                                                                 >  1:1051230/1‑139 (MQ=255)                      cgcgACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                       <  2:198360/130‑1 (MQ=255)                      cgcgACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                       >  1:198360/1‑130 (MQ=255)                               cTGGATCAACTGTCGCAAAAATAAGGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGAc                                                                                                     <  2:1051230/139‑1 (MQ=255)                                    tCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAAc                                                                                                <  2:1238739/139‑1 (MQ=255)                                    tCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAAc                                                                                                <  1:629741/139‑1 (MQ=255)                                       aCTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAg                                                                                             >  1:758682/1‑139 (MQ=255)                                            cGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                       <  2:972519/108‑1 (MQ=255)                                            cGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCtt                                                                                                                       >  1:972519/1‑108 (MQ=255)                                              cAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAG‑TTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAAcc                                                                                     >  2:280303/1‑139 (MQ=255)                                                         ttCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCt                                                                              <  2:89671/136‑1 (MQ=255)                                                         ttCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCt                                                                              >  1:89671/1‑136 (MQ=255)                                                           cAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGTCAACAGCAAGACCGGGCCAATCCAGCCttt                                                                                                                      >  2:1197694/1‑94 (MQ=255)                                                           cAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCttt                                                                                                                      <  1:1197694/94‑1 (MQ=255)                                                            aaaCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGAcc                                                                                                    <  2:807154/111‑1 (MQ=255)                                                            aaaCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGAcc                                                                                                    >  1:807154/1‑111 (MQ=255)                                                            aaaCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTg                                                                        >  1:1460514/1‑139 (MQ=255)                                                             aaCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCg                                                                                                          <  2:998932/104‑1 (MQ=255)                                                             aaCGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCg                                                                                                          >  1:998932/1‑104 (MQ=255)                                                                 cTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTggcggc                                                                   <  1:176052/139‑1 (MQ=255)                                                                            cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGc                                                                                                                                   >  2:322342/1‑64 (MQ=255)                                                                            cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGc                                                                                                                                   <  1:322342/64‑1 (MQ=255)                                                                            cgcgGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAAcc                                                        <  2:1843108/139‑1 (MQ=255)                                                                                ggCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGcc                                                    <  2:758682/139‑1 (MQ=255)                                                                                   aGCGTCTTCATCAGGCAAG‑TTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCcac                                                <  1:280303/139‑1 (MQ=255)                                                                                     cGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGAttt                                                                                                               >  2:1736909/1‑75 (MQ=255)                                                                                     cGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGAttt                                                                                                               <  1:1736909/75‑1 (MQ=255)                                                                                                 gCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTAc                                                                                                                    <  2:102282/58‑1 (MQ=255)                                                                                                 gCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTAc                                                                                                                    >  1:102282/1‑58 (MQ=255)                                                                                                   aaGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTg                                                                                   >  1:676641/1‑89 (MQ=255)                                                                                                   aaGTTTCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTg                                                                                   <  2:676641/89‑1 (MQ=255)                                                                                                      tttCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAAc                                                         >  2:135865/1‑112 (MQ=255)                                                                                                      tttCACCATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAAc                                                         <  1:135865/112‑1 (MQ=255)                                                                                                           ccATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGt                                                                         <  1:1860509/91‑1 (MQ=255)                                                                                                           ccATGATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGt                                                                         >  2:1860509/1‑91 (MQ=255)                                                                                                               gATCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATc                     >  2:586186/1‑139 (MQ=255)                                                                                                                aTCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAAc                                                         >  1:422151/1‑102 (MQ=255)                                                                                                                aTCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAAc                                                         <  2:422151/102‑1 (MQ=255)                                                                                                                aTCATTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATcc                    >  2:1644643/1‑139 (MQ=255)                                                                                                                   aTTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCt                                                                              <  1:1099215/78‑1 (MQ=255)                                                                                                                   aTTCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCt                                                                              >  2:1099215/1‑78 (MQ=255)                                                                                                                    ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAAcc                                                                                     <  1:213979/70‑1 (MQ=255)                                                                                                                    ttCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAAcc                                                                                     >  2:213979/1‑70 (MQ=255)                                                                                                                     tCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAAc                                                                                                >  2:65019/1‑58 (MQ=255)                                                                                                                     tCCAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAAc                                                                                                <  1:65019/58‑1 (MQ=255)                                                                                                                      ccAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCca                                                 >  1:1111281/1‑104 (MQ=255)                                                                                                                      ccAGCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCca                                                 <  2:1111281/104‑1 (MQ=255)                                                                                                                         gCCAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACgg           >  1:941157/1‑139 (MQ=255)                                                                                                                          ccAACGGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGAACCAACCt                                                                                    >  1:619463/1‑65 (MQ=255)                                                                                                                          ccAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCt                                                                                    <  2:619463/65‑1 (MQ=255)                                                                                                                          ccAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCg                        >  1:1582612/1‑125 (MQ=255)                                                                                                                          ccAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCg                        <  2:1582612/125‑1 (MQ=255)                                                                                                                          ccAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGc          >  2:1750817/1‑139 (MQ=255)                                                                                                                          ccAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGc          >  1:953914/1‑139 (MQ=255)                                                                                                                          ccAACAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGc          >  1:721905/1‑139 (MQ=255)                                                                                                                            aaCAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCtt        >  2:1362866/1‑139 (MQ=255)                                                                                                                              cAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTg       >  2:1588007/1‑138 (MQ=255)                                                                                                                              cAGCAAGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTg       <  1:1588007/138‑1 (MQ=255)                                                                                                                                  aaGACCGGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGaaa  <  2:721905/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GCCATTTCTGTTACCGGTTTTCGCGACAAGCTGGATCAACTGTCGCAAAAATAATGTTCAAACGCTGCCCGACAGCGCGGGCAGCGTCTTCATCAGGCAAGTTTCACCATGATCATTCCAGCCAACAGCAAGACCAGGCCAATCCAGCCTTTACGATTTAACCGTTGACCAAACAAGATCCAACCTGCGGCTAACGTGGCGGCAATACCAAACCCGCCCCACAATGCATAAGCGACAGACAAGTCGATCCCTTTAACGGCTTGAGAAA  >  NC_000913/1672728‑1672995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑