Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,726,622	G→A	*200* (TAG→TAA)	nemR →	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,726,622	0	G	A	100.0%	211.4 / NA	64	*200* (TAG→TAA)	nemR	transcriptional repressor for the nemRA‑gloA operon, quinone‑, glyoxal‑, and HOCl‑activated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/36);  total (28/36)

CCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCG  >  NC_000913/1726488‑1726747                                                                                                                                       |                                                                                                                              ccGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAgg                                                                                                                           <  2:1766460/139‑1 (MQ=255) ccGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAgg                                                                                                                           <  1:342998/139‑1 (MQ=255)   gCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCa                                                                                                                         <  1:1777395/139‑1 (MQ=255)    cTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCAt                                                                                                                        <  2:1574921/139‑1 (MQ=255)         aCAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCAtttttt                                                                                                                   <  2:1516197/139‑1 (MQ=255)                                   ggCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTAt                                                                                         <  1:452392/139‑1 (MQ=255)                                          cgcgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCAt                                                                                                                        >  1:540972/1‑101 (MQ=255)                                          cgcgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCAt                                                                                                                        <  2:540972/101‑1 (MQ=255)                                           gcgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTg                                                                                 <  2:1119152/139‑1 (MQ=255)                                           gcgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTg                                                                                 <  2:329957/139‑1 (MQ=255)                                            cgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTAt                                                                                                                 >  2:486823/1‑106 (MQ=255)                                            cgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTAt                                                                                                                 <  1:486823/106‑1 (MQ=255)                                            cgAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGa                                                                                >  1:561088/1‑139 (MQ=255)                                              aaTCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGaaa                                                                              <  2:561088/139‑1 (MQ=255)                                              aaTCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGaaa                                                                              >  1:1527572/1‑139 (MQ=255)                                                        ccAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTc                                                                    <  2:288763/139‑1 (MQ=255)                                                                   cgcAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAgg                                                                                                                           >  1:1640315/1‑73 (MQ=255)                                                                   cgcAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAgg                                                                                                                           <  2:1640315/73‑1 (MQ=255)                                                                     cAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTg                                                       <  2:1355478/139‑1 (MQ=255)                                                                                  cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAgac                                                                                                         <  2:798371/76‑1 (MQ=255)                                                                                  cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAgac                                                                                                         >  1:798371/1‑76 (MQ=255)                                                                                  cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCggg                                                                                                  <  2:681840/83‑1 (MQ=255)                                                                                  cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCggg                                                                                                  >  1:681840/1‑83 (MQ=255)                                                                                  cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAACACTGTATTCCCCACTGAAAGTGGGCGCGATCAcgg                                          >  2:1132918/1‑139 (MQ=255)                                                                                  cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACt                                                              <  1:369364/119‑1 (MQ=255)                                                                                  cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACt                                                              >  2:369364/1‑119 (MQ=255)                                                                                  cTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCAcgg                                          >  1:849165/1‑139 (MQ=255)                                                                                   tGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCAt                                                                                                                        >  1:661363/1‑60 (MQ=255)                                                                                   tGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCAt                                                                                                                        <  2:661363/60‑1 (MQ=255)                                                                                          cgcgCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCa                                                                                                                         >  1:834370/1‑52 (MQ=255)                                                                                          cgcgCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCa                                                                                                                         <  2:834370/52‑1 (MQ=255)                                                                                             gcTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGt                               >  1:878387/1‑139 (MQ=255)                                                                                               tGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCAt                                                                                                                        >  2:122196/1‑48 (MQ=255)                                                                                               tGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCAt                                                                                                                        <  1:122196/48‑1 (MQ=255)                                                                                               tGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTAt                             >  1:1353617/1‑139 (MQ=255)                                                                                                                attattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGa                                                                                                 <  2:990258/54‑1 (MQ=255)                                                                                                                attattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGa                                                                                                 >  1:990258/1‑54 (MQ=255)                                                                                                                attattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGa            <  2:1056200/139‑1 (MQ=255)                                                                                                                attattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGa            >  1:1056200/1‑139 (MQ=255)                                                                                                                 ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGAc           >  2:1735107/1‑139 (MQ=255)                                                                                                                 ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCCTTATGTc                                                                                      <  2:1732938/64‑1 (MQ=255)                                                                                                                 ttattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCCTTATGTc                                                                                      >  1:1732938/1‑64 (MQ=255)                                                                                                                  tattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTcc                                                                   >  2:1876530/1‑82 (MQ=255)                                                                                                                  tattGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTcc                                                                   <  1:1876530/82‑1 (MQ=255)                                                                                                                     tGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTg                                                       <  2:31451/91‑1 (MQ=255)                                                                                                                     tGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTg                                                       >  1:31451/1‑91 (MQ=255)                                                                                                                     tGCGACGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGGGGGCGGGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGt       <  1:550508/139‑1 (MQ=255)                                                                                                                         aCGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCgg                                                                                                   <  1:462929/43‑1 (MQ=255)                                                                                                                         aCGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCgg                                                                                                   >  2:462929/1‑43 (MQ=255)                                                                                                                         aCGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTc                                                                    >  2:83732/1‑74 (MQ=255)                                                                                                                         aCGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTc                                                                    <  1:83732/74‑1 (MQ=255)                                                                                                                         aCGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgc   >  1:970409/1‑139 (MQ=255)                                                                                                                          cGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg  <  2:1527572/139‑1 (MQ=255)                                                                                                                          cGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg  <  2:1353617/139‑1 (MQ=255)                                                                                                                          cGCCTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg  <  1:1735107/139‑1 (MQ=255)                                                                                                                          cGCATGCCGTTGAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTgcg  <  2:1218654/139‑1 (MQ=255)                                                                                                                            ccTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGaaaa                                                                             >  1:1873361/1‑62 (MQ=255)                                                                                                                            ccTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGaaaa                                                                             <  2:1873361/62‑1 (MQ=255)                                                                                                                             cTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACggcggc                                      <  2:69849/100‑1 (MQ=255)                                                                                                                             cTGCCGTTTAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACggcggc                                      >  1:69849/1‑100 (MQ=255)                                                                                                                                ccGTTTAACAGGCATTTTTTATCACCAGACGACCggg                                                                                                  <  1:1416260/37‑1 (MQ=255)                                                                                                                                ccGTTTAACAGGCATTTTTTATCACCAGACGACCggg                                                                                                  >  2:1416260/1‑37 (MQ=255)                                                                                                                                   tttAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCa                                     <  1:960904/95‑1 (MQ=255)                                                                                                                                   tttAACAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCa                                     >  2:960904/1‑95 (MQ=255)                                                                                                                                       |                                                                                                                              CCGCTGCAACAGGCACAAGTGCTTTACGCACTGTGGCTTGGCGCGAATCTGCAGGCCAAAATTTCGCGCAGTTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCACCAGACGACCGGGAGCCTTTATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAACCGTATTTTTATGGCACCGCTGACGCGTCTGCG  >  NC_000913/1726488‑1726747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑