Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,905,989	G→A	R101H (CGT→CAT)	mntP →	putative Mn(2+) efflux pump, mntR‑regulated

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,905,989	0	G	A	100.0%	245.4 / NA	75	R101H (CGT→CAT)	mntP	putative Mn(2+) efflux pump, mntR‑regulated
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/36);  total (39/36)

GGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGA  >  NC_000913/1905853‑1906123                                                                                                                                         |                                                                                                                                       ggAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATc                                                                                                                                      <  2:803930/139‑1 (MQ=255)     tGGGCCTGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTTCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAATATGAAGAGCCGCGCCATCGaca                                                                                                                                  <  2:1556408/139‑1 (MQ=255)       ggCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACg                                                                                                                                <  1:503776/139‑1 (MQ=255)          aTGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGtt                                                                                                                             >  1:415301/1‑139 (MQ=255)                gccagccGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGc                                                                                                                       <  2:1611428/139‑1 (MQ=255)                 ccagccGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCt                                                                                                                      >  2:219603/1‑139 (MQ=255)                  cagccGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTa                                                                                                                     <  2:593028/139‑1 (MQ=255)                     ccGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTg                                                                                                                  >  2:570549/1‑139 (MQ=255)                      cGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTgg                                                                                                                 >  2:1439255/1‑139 (MQ=255)                       ggTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGt                                                                                                                <  2:29970/139‑1 (MQ=255)                        gTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTa                                                                                                               >  2:389468/1‑139 (MQ=255)                                       gAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGaca                                                                                                                                  >  1:1071935/1‑105 (MQ=255)                                       gAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGaca                                                                                                                                  <  2:1071935/105‑1 (MQ=255)                                       gAACCACTGGATTGCGTTTGAGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGcc                                                                                                >  1:1105010/1‑139 (MQ=255)                                           cACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGccacca                                                                                            <  1:389468/139‑1 (MQ=255)                                                  ttGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGa                                                                                     >  2:192903/1‑139 (MQ=255)                                                  ttGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGa                                                                                     <  2:1105010/139‑1 (MQ=255)                                                  ttGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGa                                                                                     <  1:192903/139‑1 (MQ=255)                                                     cGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGc                                                                                  >  2:1478944/1‑139 (MQ=255)                                                     cGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGc                                                                                  >  2:715415/1‑139 (MQ=255)                                                          gtgCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGGTGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGc                                                                                                                       >  2:1731650/1‑97 (MQ=255)                                                          gtgCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGGTGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGc                                                                                                                       <  1:1731650/97‑1 (MQ=255)                                                                ctgATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATc                                                                                                                                      >  2:430639/1‑76 (MQ=255)                                                                ctgATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATc                                                                                                                                      <  1:430639/76‑1 (MQ=255)                                                                ctgATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTgg                                                                       >  1:118189/1‑139 (MQ=255)                                                                     atTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGtt                                                                  >  1:457348/1‑139 (MQ=255)                                                                        ccTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGt                                                               >  1:190563/1‑139 (MQ=255)                                                                          tCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCt                                                             >  2:1505151/1‑139 (MQ=255)                                                                          tCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCt                                                             >  1:1285158/1‑139 (MQ=255)                                                                             gcgggcgAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGc                                                                                                                       >  2:735771/1‑78 (MQ=255)                                                                             gcgggcgAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGc                                                                                                                       <  1:735771/78‑1 (MQ=255)                                                                              cgggcgAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCt                                                         <  1:219603/139‑1 (MQ=255)                                                                                   gAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCt                                                    <  1:894979/139‑1 (MQ=255)                                                                                    aaTGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTg                                                   <  2:190563/139‑1 (MQ=255)                                                                                           attGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCa                                            >  2:1813910/1‑139 (MQ=255)                                                                                               aGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACAt                                        >  1:745097/1‑139 (MQ=255)                                                                                                gggTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACAtt                                       >  1:891458/1‑139 (MQ=255)                                                                                                 ggTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGtt                                                                  >  1:155279/1‑111 (MQ=255)                                                                                                 ggTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGtt                                                                  <  2:155279/111‑1 (MQ=255)                                                                                                 ggTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGt                                              >  2:295670/1‑131 (MQ=255)                                                                                                 ggTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGt                                              <  1:295670/131‑1 (MQ=255)                                                                                                 ggTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTa                                      >  1:1438776/1‑139 (MQ=255)                                                                                                 ggTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGGCAACATTa                                      >  1:330876/1‑139 (MQ=255)                                                                                                  gTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTAt                                     >  2:1016379/1‑139 (MQ=255)                                                                                                  gTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTAt                                     >  1:604394/1‑139 (MQ=255)                                                                                                   ttttCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATc                                    >  1:1593096/1‑139 (MQ=255)                                                                                                    tttCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATcg                                   >  1:464900/1‑139 (MQ=255)                                                                                                    tttCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATcg                                   >  1:242380/1‑139 (MQ=255)                                                                                                       cGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACcg                                                                                                        >  2:255247/1‑67 (MQ=255)                                                                                                       cGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACcg                                                                                                        <  1:255247/67‑1 (MQ=255)                                                                                                       cGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGa                                <  1:419054/139‑1 (MQ=255)                                                                                                           gcgcAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTg                                                           >  2:688043/1‑108 (MQ=255)                                                                                                           gcgcAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTg                                                           <  1:688043/108‑1 (MQ=255)                                                                                                             gcAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCAt                          <  2:1593096/139‑1 (MQ=255)                                                                                                             gcAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCAt                          <  2:1438776/139‑1 (MQ=255)                                                                                                             gcAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCAt                          <  2:415301/139‑1 (MQ=255)                                                                                                              cAGATGATGAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCAtt                         <  1:570549/139‑1 (MQ=255)                                                                                                                 atgatgAAGATGAAGAGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGc                      >  1:834347/1‑139 (MQ=255)                                                                                                                     tgaagatgaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCAtt                  <  1:1478944/139‑1 (MQ=255)                                                                                                                      gaagatgaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGt                                              <  2:1509461/110‑1 (MQ=255)                                                                                                                      gaagatgaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGt                                              >  1:1509461/1‑110 (MQ=255)                                                                                                                      gaagatgaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTg                 <  2:891458/139‑1 (MQ=255)                                                                                                                           tgaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgt            >  1:325416/1‑139 (MQ=255)                                                                                                                            gaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg           <  2:55456/139‑1 (MQ=255)                                                                                                                            gaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg           <  1:1813910/139‑1 (MQ=255)                                                                                                                            gaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg           <  2:604394/139‑1 (MQ=255)                                                                                                                            gaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg           >  1:55456/1‑139 (MQ=255)                                                                                                                            gaagaGCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTtgtg           <  2:745097/139‑1 (MQ=255)                                                                                                                                 gCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAAcc      <  2:457348/139‑1 (MQ=255)                                                                                                                                 gCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAAcc      <  2:118189/139‑1 (MQ=255)                                                                                                                                 gCCGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAAcc      <  2:842572/139‑1 (MQ=255)                                                                                                                                  ccGCGCCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCt     >  2:960233/1‑139 (MQ=255)                                                                                                                                    gcgcCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGc                                                  >  1:887402/1‑92 (MQ=255)                                                                                                                                    gcgcCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGc                                                  <  2:887402/92‑1 (MQ=255)                                                                                                                                     cgcCATCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGa  >  2:41439/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                       GGAATGGGCATGTTAGCCAGCCGGTTTGTCCTTGAATGGAACCACTGGATTGCGTTTGTGCTGCTGATATTCCTCGGCGGGCGAATGATTATTGAGGGTTTTCGTGGCGCAGATGATGAAGATGAAGAGCCGCGCCGTCGACACGGTTTCTGGCTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGA  >  NC_000913/1905853‑1906123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑