Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,153,669 G→A noncoding (26/136 nt) sibB → sRNA antisense regulator of toxic IbsB protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,153,6690GA100.0% 336.0 / NA 101noncoding (26/136 nt)sibBsRNA antisense regulator of toxic IbsB protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (53/48);  total (53/48)

Only 100 of 101 total aligned reads displayed.

TTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAA  >  NC_000913/2153535‑2153802
                                                                                                                                      |                                                                                                                                     
tttCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCt                                                                                                                                   >  2:1238470/1‑139 (MQ=255)
tttCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCt                                                                                                                                   >  1:1397713/1‑139 (MQ=255)
 ttCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTg                                                                                                                                  >  1:610299/1‑139 (MQ=255)
  tCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 <  2:1680742/139‑1 (MQ=255)
  tCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 >  2:761102/1‑139 (MQ=255)
    ctctGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 <  1:878908/137‑1 (MQ=255)
    ctctGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 >  2:878908/1‑137 (MQ=255)
     tctGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAg                                                                                                                              >  2:641028/1‑139 (MQ=255)
              attGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTaa                                                                                                                     >  2:1204552/1‑139 (MQ=255)
              attGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTaa                                                                                                                     >  2:1769970/1‑139 (MQ=255)
                   gTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCggg                                                                                                                >  1:298772/1‑139 (MQ=255)
                    tAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               <  1:761102/139‑1 (MQ=255)
                          cTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCtt                                                                                                         <  1:641028/139‑1 (MQ=255)
                          cTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCtt                                                                                                         <  1:1769970/139‑1 (MQ=255)
                           tCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTa                                                                                                        <  2:298772/139‑1 (MQ=255)
                            cGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGc                                                                                                           <  1:258225/135‑1 (MQ=255)
                            cGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGc                                                                                                           >  2:258225/1‑135 (MQ=255)
                                  gAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTaa                                                                                                 <  1:1352577/139‑1 (MQ=255)
                                   aaTATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAag                                                                                                >  1:926173/1‑139 (MQ=255)
                                     tatTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAagag                                                                                              <  2:610299/139‑1 (MQ=255)
                                          gCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGcacca                                                                                         <  1:1204552/139‑1 (MQ=255)
                                               cAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               >  1:1672368/1‑112 (MQ=255)
                                               cAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               <  2:1672368/112‑1 (MQ=255)
                                                aGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACgatga                                                                                   >  2:628352/1‑139 (MQ=255)
                                                 ggATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCtt                                                                                                         <  2:437777/116‑1 (MQ=255)
                                                 ggATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCtt                                                                                                         >  1:437777/1‑116 (MQ=255)
                                                     cGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 <  2:189276/88‑1 (MQ=255)
                                                     cGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 >  1:189276/1‑88 (MQ=255)
                                                          aCTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCtt                                                                         <  2:1446317/139‑1 (MQ=255)
                                                            tACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAagag                                                                                              <  1:1536513/116‑1 (MQ=255)
                                                            tACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAagag                                                                                              >  2:1536513/1‑116 (MQ=255)
                                                              cAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 >  1:754403/1‑79 (MQ=255)
                                                              cAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 <  2:754403/79‑1 (MQ=255)
                                                              cAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatc                                                                     >  1:146780/1‑139 (MQ=255)
                                                              cAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatc                                                                     >  1:136514/1‑139 (MQ=255)
                                                               aaaGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatca                                                                    <  2:926173/139‑1 (MQ=255)
                                                               aaaGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatca                                                                    <  2:146780/139‑1 (MQ=255)
                                                               aaaGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatca                                                                    <  2:136514/139‑1 (MQ=255)
                                                                 aGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATg                                                                  >  1:362417/1‑139 (MQ=255)
                                                                  gCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGa                                                                 <  1:786569/139‑1 (MQ=255)
                                                                      gCGGATTGACAATCTTATCGTGAAGGCGTACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACg                                                                                       >  1:1666008/1‑113 (MQ=255)
                                                                      gCGGATTGACAATCTTATCGTGAAGGCGTACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACg                                                                                       <  2:1666008/113‑1 (MQ=255)
                                                                      gCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 >  1:766050/1‑71 (MQ=255)
                                                                      gCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 <  2:766050/71‑1 (MQ=255)
                                                                      gCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCt                                                             >  2:1765629/1‑139 (MQ=255)
                                                                      gCGGATTGACAATCTAATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCt                                                             >  1:1258099/1‑139 (MQ=255)
                                                                       cGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCtt                                                                         >  2:669054/1‑126 (MQ=255)
                                                                       cGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCtt                                                                         <  1:669054/126‑1 (MQ=255)
                                                                                    ttATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAACAGCACCACGATGATGAGTAGCTtcatca                                                                    >  1:850238/1‑118 (MQ=255)
                                                                                    ttATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAACAGCACCACGATGATGAGTAGCTtcatca                                                                    <  2:850238/118‑1 (MQ=255)
                                                                                      aTCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 <  2:1179557/55‑1 (MQ=255)
                                                                                      aTCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTgg                                                                                                                                 >  1:1179557/1‑55 (MQ=255)
                                                                                      aTCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcc                                             >  2:350724/1‑139 (MQ=255)
                                                                                      aTCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcc                                             <  2:362417/139‑1 (MQ=255)
                                                                                      aTCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcc                                             <  2:1397713/139‑1 (MQ=255)
                                                                                      aTCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcc                                             <  1:628352/139‑1 (MQ=255)
                                                                                            aaGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTa                                                                                                                               <  1:1541562/51‑1 (MQ=255)
                                                                                            aaGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTa                                                                                                                               >  2:1541562/1‑51 (MQ=255)
                                                                                            aaGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               <  2:172323/67‑1 (MQ=255)
                                                                                            aaGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               >  1:172323/1‑67 (MQ=255)
                                                                                            aaGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCTCTTcc                                       >  2:1166584/1‑139 (MQ=255)
                                                                                            aaGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTcc                                       >  2:569873/1‑139 (MQ=255)
                                                                                             aGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCt                                      <  2:443646/139‑1 (MQ=255)
                                                                                              ggCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcc                                             <  2:809321/131‑1 (MQ=255)
                                                                                              ggCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcc                                             >  1:809321/1‑131 (MQ=255)
                                                                                                 aTACTTTCAGGAGTGAGGATAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcccc                                           <  1:1312862/130‑1 (MQ=255)
                                                                                                 aTACTTTCAGGAGTGAGGATAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcccc                                           >  2:1312862/1‑130 (MQ=255)
                                                                                                     tttCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatca                                                                    >  2:1379707/1‑101 (MQ=255)
                                                                                                     tttCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTtcatca                                                                    <  1:1379707/101‑1 (MQ=255)
                                                                                                         aGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGatgatg                                                                                 <  2:463677/84‑1 (MQ=255)
                                                                                                         aGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGatgatg                                                                                 >  1:463677/1‑84 (MQ=255)
                                                                                                         aGGAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCtt                          >  1:1712151/1‑139 (MQ=255)
                                                                                                           gAGTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTc                        <  2:921526/139‑1 (MQ=255)
                                                                                                             gTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGAcc                                                               >  1:358829/1‑98 (MQ=255)
                                                                                                             gTGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGAcc                                                               <  2:358829/98‑1 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               >  2:902591/1‑49 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               <  1:902591/49‑1 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTa                                                                                                        <  1:380004/56‑1 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTa                                                                                                        >  2:380004/1‑56 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAg                                                                               <  2:790204/81‑1 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAg                                                                               >  1:790204/1‑81 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCtta                                                      <  1:1802646/106‑1 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCtta                                                      >  2:1802646/1‑106 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTcc                       <  2:643086/137‑1 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTcc                       >  1:643086/1‑137 (MQ=255)
                                                                                                              tGAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCg                     >  2:740201/1‑139 (MQ=255)
                                                                                                               gAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGaa                                                                                                             <  2:1432366/50‑1 (MQ=255)
                                                                                                               gAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGaa                                                                                                             >  1:1432366/1‑50 (MQ=255)
                                                                                                               gAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGcacca                                                                                         >  2:1649933/1‑70 (MQ=255)
                                                                                                               gAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGcacca                                                                                         <  1:1649933/70‑1 (MQ=255)
                                                                                                               gAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcc                                             >  1:1671965/1‑114 (MQ=255)
                                                                                                               gAGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGcc                                             <  2:1671965/114‑1 (MQ=255)
                                                                                                                aGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGtt                   >  1:577601/1‑139 (MQ=255)
                                                                                                                aGGGTAGAGCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGtt                   >  1:140935/1‑139 (MQ=255)
                                                                                                                       agCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               >  2:339256/1‑40 (MQ=255)
                                                                                                                       agCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCgggg                                                                                                               <  1:339256/40‑1 (MQ=255)
                                                                                                                       agCGGGGTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGt                                                                              >  1:95334/1‑73 (MQ=255)
                                                                                                                            ggTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGca                                                                                            <  2:1197099/54‑1 (MQ=255)
                                                                                                                            ggTTTCCCCCACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGca                                                                                            >  1:1197099/1‑54 (MQ=255)
                                                                                                                                 cccccACCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGaaa  >  2:823102/1‑139 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TTTCCTCTGTGATTATTGCGTAACGACTCGCAAAGAATATTTGCTGGCAGGATCGCAGACTACAAAGCCTGCGGATTGACAATCTTATCGTGAAGGCATACTTTCAGGAGTGAGGGTAGAGCGGGGTTTCCCCCGCCCTGGTAGTCTTAGTAAGCGGGGAAGCTTATGACTAAGAGCACCACGATGATGAGTAGCTTCATCATGACCCTTTCCTTATTTATGGCCCCTTCCTCGGGAGGGGCTTTCCCGTTTCAGCGTCCCGCTGAAA  >  NC_000913/2153535‑2153802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: