Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,226,509	C→T	*254* (TAG→TAA)	yohF ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,226,509	0	C	T	100.0%	164.5 / NA	51	*254* (TAG→TAA)	yohF	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/25);  total (26/25)

ATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCA  >  NC_000913/2226384‑2226638                                                                                                                              |                                                                                                                                  aTTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCG‑CTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGaa                                                                                                                     <  1:429986/139‑1 (MQ=255)    gCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                               <  1:160865/127‑1 (MQ=255)    gCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTAtt                                                                                                                               >  2:160865/1‑127 (MQ=255)         ggATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                              <  1:1520509/139‑1 (MQ=255)               ggCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCCAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGcc                                                                                                        >  2:1362086/1‑139 (MQ=255)                     cgcgCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGc                                                                                                         <  2:938396/132‑1 (MQ=255)                     cgcgCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGc                                                                                                         >  1:938396/1‑132 (MQ=255)                                  aCATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                     <  1:1435902/139‑1 (MQ=255)                                         gAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATc                                                                                       <  1:467908/130‑1 (MQ=255)                                         gAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATc                                                                                       >  2:467908/1‑130 (MQ=255)                                         gAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCaa                                                                              >  1:433143/1‑139 (MQ=255)                                               cATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTg                                                                        >  2:1748993/1‑139 (MQ=255)                                                 tGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                            <  2:406285/117‑1 (MQ=255)                                                 tGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                            >  1:406285/1‑117 (MQ=255)                                                        tACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg                                                                   >  2:193516/1‑135 (MQ=255)                                                        tACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCgg                                                                   <  1:193516/135‑1 (MQ=255)                                                                 tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgc                                                      <  2:1181045/139‑1 (MQ=255)                                                                 tgtgCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTgc                                                      <  1:1748993/139‑1 (MQ=255)                                                                                    aCCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGc                                                                                                         >  1:1179658/1‑69 (MQ=255)                                                                                    aCCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGc                                                                                                         <  2:1179658/69‑1 (MQ=255)                                                                                      cAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCacac                                 <  2:1850362/139‑1 (MQ=255)                                                                                                cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                            <  2:1453242/70‑1 (MQ=255)                                                                                                cccAATGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAgccg                                                                                            >  1:1453242/1‑70 (MQ=255)                                                                                                   aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                              <  2:1399129/49‑1 (MQ=255)                                                                                                   aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                              >  1:1399129/1‑49 (MQ=255)                                                                                                   aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                     <  1:1447093/74‑1 (MQ=255)                                                                                                   aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCa                                                                                     >  2:1447093/1‑74 (MQ=255)                                                                                                   aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTgg                      >  1:1731663/1‑137 (MQ=255)                                                                                                   aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTgg                      <  2:1731663/137‑1 (MQ=255)                                                                                                   aaTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCa                    >  2:1069471/1‑139 (MQ=255)                                                                                                    aTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                              >  1:667415/1‑48 (MQ=255)                                                                                                    aTGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGa                                                                                                              <  2:667415/48‑1 (MQ=255)                                                                                                     tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGccc                                                                     >  2:730380/1‑88 (MQ=255)                                                                                                     tGAAATATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGccc                                                                     <  1:730380/88‑1 (MQ=255)                                                                                                        aaTATCCACGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAAtctc               >  2:1338240/1‑139 (MQ=255)                                                                                                               aCGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCca                                    >  1:361735/1‑111 (MQ=255)                                                                                                               aCGCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCca                                    <  2:361735/111‑1 (MQ=255)                                                                                                                 gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                            >  1:283784/1‑101 (MQ=255)                                                                                                                 gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                            <  1:196749/101‑1 (MQ=255)                                                                                                                 gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                            >  2:196749/1‑101 (MQ=255)                                                                                                                 gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGaa                                            <  2:283784/101‑1 (MQ=255)                                                                                                                 gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCa                    >  1:59101/1‑125 (MQ=255)                                                                                                                 gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCa                    <  2:59101/125‑1 (MQ=255)                                                                                                                 gCACCTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTgc      >  2:1635381/1‑139 (MQ=255)                                                                                                                     cTCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCa  >  2:1080974/1‑139 (MQ=255)                                                                                                                      tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAg                           <  1:176266/113‑1 (MQ=255)                                                                                                                      tCACTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAg                           >  2:176266/1‑113 (MQ=255)                                                                                                                        aCTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATc                                                                                       >  1:1850943/1‑51 (MQ=255)                                                                                                                        aCTGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATc                                                                                       <  2:1850943/51‑1 (MQ=255)                                                                                                                          tGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc                                                                      <  2:356995/66‑1 (MQ=255)                                                                                                                          tGCGTTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGcc                                                                      >  1:356995/1‑66 (MQ=255)                                                                                                                              |                                                                                                                                  ATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTGGATTCGCCAACATAAAGCCGCCATCCACTATCAACGACTGCCCGGTGGTGTAATTTGCGCCCTCCGAACAAAGCCACACCACCAGGCTGGCAATCTCATGCGTTGCGCCA  >  NC_000913/2226384‑2226638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑