Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 219,364 T→C P77P (CCA→CCG)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913219,3640TC100.0% 200.4 / NA 56P77P (CCA→CCG) tsaAtRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/24);  total (32/24)

CCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGG  >  NC_000913/219230‑219500
                                                                                                                                      |                                                                                                                                        
ccGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGAc                                                                                                                                      >  1:997148/1‑139 (MQ=255)
ccGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGAc                                                                                                                                      >  1:1367622/1‑139 (MQ=255)
ccGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGAc                                                                                                                                      >  2:1750745/1‑139 (MQ=255)
ccGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGAc                                                                                                                                      >  1:1796854/1‑139 (MQ=255)
   aGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGca                                                                                                                                   <  2:997148/139‑1 (MQ=255)
    gCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGcac                                                                                                                                  <  2:1367622/139‑1 (MQ=255)
                 gCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                     <  2:1583637/139‑1 (MQ=255)
                     tCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgccgc                                                                                                                 >  2:881919/1‑139 (MQ=255)
                         tATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTc                                                                                                                              <  1:924463/122‑1 (MQ=255)
                         tATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTc                                                                                                                              >  2:924463/1‑122 (MQ=255)
                          aTGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTcc                                                                                                            <  1:1786195/139‑1 (MQ=255)
                             gCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCTATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATc                                                                                                         >  1:604980/1‑139 (MQ=255)
                               aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                                       >  2:1661808/1‑139 (MQ=255)
                               aaaCAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                                       >  1:100709/1‑139 (MQ=255)
                                 acaacCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGttt                                                                                                     >  2:1864695/1‑139 (MQ=255)
                                     ccTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                     >  2:716223/1‑119 (MQ=255)
                                     ccTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                     <  1:716223/119‑1 (MQ=255)
                                               ctctACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaa                                                                                       <  1:1467190/139‑1 (MQ=255)
                                                tctACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGaaaa                                                                                      <  2:100709/139‑1 (MQ=255)
                                                  tACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAgg                                                                                    <  1:1864695/139‑1 (MQ=255)
                                                    ccAGCGACATGCCTATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                                  <  2:604980/139‑1 (MQ=255)
                                                    ccAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                                  <  2:1796854/139‑1 (MQ=255)
                                                         gACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATa                                                                             >  1:689466/1‑139 (MQ=255)
                                                              gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                                       <  2:1028306/108‑1 (MQ=255)
                                                              gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGt                                                                                                       >  1:1028306/1‑108 (MQ=255)
                                                              gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATgg                                                                        >  2:1028893/1‑139 (MQ=255)
                                                              gCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATgg                                                                        >  2:159733/1‑139 (MQ=255)
                                                                   ttGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                   <  1:881919/139‑1 (MQ=255)
                                                                   ttGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                   >  2:1108956/1‑139 (MQ=255)
                                                                      ggTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGc                                                                >  2:516964/1‑139 (MQ=255)
                                                                            ggCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTGACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAg                                                          >  2:1228511/1‑139 (MQ=255)
                                                                                gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                                  <  2:288908/111‑1 (MQ=255)
                                                                                gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                                  >  1:288908/1‑111 (MQ=255)
                                                                                gAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCcg                                                      >  1:826727/1‑139 (MQ=255)
                                                                                             gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACCGTCGGACGCCAgccg                                                                                                                  <  1:1258161/66‑1 (MQ=255)
                                                                                             gtTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCACGGCGGACGCACAGTCGGACGCCAgccg                                                                                                                  >  2:1258161/1‑66 (MQ=255)
                                                                                               tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                   >  1:371820/1‑111 (MQ=255)
                                                                                               tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                   <  2:371820/111‑1 (MQ=255)
                                                                                               tGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGcc                                       <  2:826727/139‑1 (MQ=255)
                                                                                                   aaaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGc                                        >  2:1407383/1‑134 (MQ=255)
                                                                                                   aaaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGc                                        <  1:1407383/134‑1 (MQ=255)
                                                                                                    aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                                                   >  2:767727/1‑58 (MQ=255)
                                                                                                    aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                                                   <  1:767727/58‑1 (MQ=255)
                                                                                                    aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                     >  2:687866/1‑56 (MQ=255)
                                                                                                    aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAg                                                                                                                     <  1:687866/56‑1 (MQ=255)
                                                                                                    aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                   >  1:1666847/1‑106 (MQ=255)
                                                                                                    aaaCCCCCATTCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGaa                                                                   <  2:1666847/106‑1 (MQ=255)
                                                                                                              tCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                                                   <  2:1045876/48‑1 (MQ=255)
                                                                                                              tCTGGCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAgcc                                                                                                                   >  1:1045876/1‑48 (MQ=255)
                                                                                                                  gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                                  >  1:1725563/1‑77 (MQ=255)
                                                                                                                  gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGAt                                                                                  <  2:1725563/77‑1 (MQ=255)
                                                                                                                  gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATga                    >  1:480428/1‑139 (MQ=255)
                                                                                                                  gCGTTACCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATga                    >  1:686103/1‑139 (MQ=255)
                                                                                                                       aCCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAg                                                          <  1:1581981/96‑1 (MQ=255)
                                                                                                                       aCCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAg                                                          >  2:1581981/1‑96 (MQ=255)
                                                                                                                       aCCGCCGAGGCGCGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGc               >  2:701657/1‑139 (MQ=255)
                                                                                                                                gcgcGGCGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCg      >  2:1197640/1‑139 (MQ=255)
                                                                                                                                   cggcggACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTA‑GGAGCAATGAGATGCAGTTCGCCGTTgg  <  1:1750745/139‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CCGAGCTTCAGAATCACGCTGTCTTTATGGCAAACAACCTCTTTCAGCTCTACCAGCGACATGCCAATTGGGTTAGGGCGGAAAGTAGAGCGTGTTGCGAAAACCCCCATTCTGGCGTTACCGCCGAGGCGCGGTGGACGCACAGTCGGACGCCAGCCGCCTTCCATCGTTTGATGAAAGACGAAAAGGATCCATAAATGGCTGAACGCTTCCAGGCCGCGAACGGCGTCGGCCTGGTTGTAGGGAGCAATGAGATGCAGTTCGCCGTTGG  >  NC_000913/219230‑219500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑