Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,274,166	A→G	K601K (AAA→AAG)	yejA →	microcin C ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,166	0	A	G	100.0%	297.1 / NA	82	K601K (AAA→AAG)	yejA	microcin C ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/40);  total (42/40)

GTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGC  >  NC_000913/2274034‑2274300                                                                                                                                     |                                                                                                                                       gTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                                  >  2:1660867/1‑139 (MQ=255) gTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGga                                                                                                                                  <  2:390587/139‑1 (MQ=255)   aCATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGgaga                                                                                                                                >  1:346786/1‑139 (MQ=255)      tGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTa                                                                                                                             >  2:1865861/1‑139 (MQ=255)       ggCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaa                                                                                                                            >  1:532680/1‑138 (MQ=255)         cGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTaaat                                                                                                                          >  2:579256/1‑136 (MQ=255)            aaGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATggg                                                                                                                       <  1:1424448/139‑1 (MQ=255)            aaGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATggg                                                                                                                       <  1:1801491/139‑1 (MQ=255)                   tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTAc                                                                                                                >  1:725900/1‑139 (MQ=255)                   tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTAc                                                                                                                >  1:44677/1‑139 (MQ=255)                   tctcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTAc                                                                                                                >  2:1836048/1‑139 (MQ=255)                     tcGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCt                                                                                                              <  2:952532/139‑1 (MQ=255)                           ggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        <  1:1865861/139‑1 (MQ=255)                           ggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        <  2:1788991/139‑1 (MQ=255)                           ggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        <  2:453575/139‑1 (MQ=255)                           ggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        >  1:365517/1‑139 (MQ=255)                           ggtggGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        <  2:596449/139‑1 (MQ=255)                              gggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCg                                                                                                     <  1:617732/139‑1 (MQ=255)                               ggATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGt                                                                                                    <  2:365517/139‑1 (MQ=255)                                 aTAAATTCTCCCAGCCGGCCGTGCGCCCTATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAgg                                                                                                                                    <  1:1345187/105‑1 (MQ=255)                                 aTAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAgg                                                                                                                                    >  2:1345187/1‑105 (MQ=255)                                 aTAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCt                                                                                                  <  2:44677/139‑1 (MQ=255)                                         tcCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg                                                                                          <  1:579256/139‑1 (MQ=255)                                          cccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         <  1:1660867/139‑1 (MQ=255)                                           ccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         >  2:265377/1‑138 (MQ=255)                                           ccAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         <  1:265377/138‑1 (MQ=255)                                                cggccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                   <  2:532680/139‑1 (MQ=255)                                                  gccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         >  2:1126092/1‑131 (MQ=255)                                                  gccgTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         <  1:1126092/131‑1 (MQ=255)                                                        cgcCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTAt                                                                           >  2:105617/1‑139 (MQ=255)                                                                    aGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCagcaagca                                                                                                                                      <  1:571619/68‑1 (MQ=255)                                                                     gCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCAc                                                              >  2:1035982/1‑139 (MQ=255)                                                                        tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         <  2:202218/109‑1 (MQ=255)                                                                        tCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         >  1:202218/1‑109 (MQ=255)                                                                         cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAg                                                                                                                                     >  1:743459/1‑64 (MQ=255)                                                                         cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAg                                                                                                                                     <  2:743459/64‑1 (MQ=255)                                                                         cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATg                                                                                                                         >  1:764092/1‑76 (MQ=255)                                                                         cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATg                                                                                                                         <  2:764092/76‑1 (MQ=255)                                                                         cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                          >  1:1723222/1‑139 (MQ=255)                                                                         cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                          <  2:1723222/139‑1 (MQ=255)                                                                         cGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATc                                                          >  2:1604561/1‑139 (MQ=255)                                                                          ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCagcaagca                                                                                                                                      >  1:1517991/1‑62 (MQ=255)                                                                          ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTg                                                                                                 >  2:353118/1‑99 (MQ=255)                                                                          ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTg                                                                                                 <  1:353118/99‑1 (MQ=255)                                                                          ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg                                                                                          >  1:83658/1‑106 (MQ=255)                                                                          ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg                                                                                          <  2:83658/106‑1 (MQ=255)                                                                          ggTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCa                                                         >  2:1677500/1‑139 (MQ=255)                                                                           gTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCaa                                                        >  2:840075/1‑139 (MQ=255)                                                                                 aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGTCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         <  1:459672/100‑1 (MQ=255)                                                                                 aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGTCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         >  2:459672/1‑100 (MQ=255)                                                                                 aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGc                                                                                                       >  2:1033999/1‑86 (MQ=255)                                                                                 aTACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGc                                                                                                       <  1:1033999/86‑1 (MQ=255)                                                                                    ccTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         <  1:882736/97‑1 (MQ=255)                                                                                    ccTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         >  2:882736/1‑97 (MQ=255)                                                                                      tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATc                                                                                     >  2:76488/1‑99 (MQ=255)                                                                                      tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATc                                                                                     <  1:76488/99‑1 (MQ=255)                                                                                        gtggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTg                                           >  2:923065/1‑139 (MQ=255)                                                                                              tGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        >  1:1323851/1‑72 (MQ=255)                                                                                              tGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        <  2:1323851/72‑1 (MQ=255)                                                                                                  gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTgcgc                                 >  1:1857533/1‑139 (MQ=255)                                                                                                   tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        >  2:1380729/1‑67 (MQ=255)                                                                                                   tCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCg                                                                                                        <  1:1380729/67‑1 (MQ=255)                                                                                                     aaTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                   >  2:1399453/1‑86 (MQ=255)                                                                                                     aaTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                   <  1:1399453/86‑1 (MQ=255)                                                                                                      aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         <  1:937521/79‑1 (MQ=255)                                                                                                      aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGt                                                                                         >  2:937521/1‑79 (MQ=255)                                                                                                      aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTgg                             <  1:646185/139‑1 (MQ=255)                                                                                                      aTAAAGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTgg                             >  1:930484/1‑139 (MQ=255)                                                                                                          aGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTc                                                                                                   >  1:832553/1‑65 (MQ=255)                                                                                                          aGCGGCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTc                                                                                                   <  2:832553/65‑1 (MQ=255)                                                                                                             ggCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTAt                                                                           >  2:1869356/1‑86 (MQ=255)                                                                                                             ggCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTAt                                                                           <  1:1869356/86‑1 (MQ=255)                                                                                                              gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                   >  2:390579/1‑77 (MQ=255)                                                                                                              gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                   <  1:390579/77‑1 (MQ=255)                                                                                                              gCCAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccgg                     <  2:930484/139‑1 (MQ=255)                                                                                                               ccAAACTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGcggtccggt                    >  2:789334/1‑139 (MQ=255)                                                                                                                   aCTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCAt                                                           >  1:478936/1‑96 (MQ=255)                                                                                                                   aCTGCCGTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCAt                                                           <  2:478936/96‑1 (MQ=255)                                                                                                                         gTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                   <  2:1641174/66‑1 (MQ=255)                                                                                                                         gTCCGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATccc                                                                                   >  1:1641174/1‑66 (MQ=255)                                                                                                                           ccGCCAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCAt        <  1:789334/139‑1 (MQ=255)                                                                                                                               cAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg                                                                                          <  2:1845758/53‑1 (MQ=39)                                                                                                                               cAGCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTgg                                                                                          >  1:1845758/1‑53 (MQ=39)                                                                                                                                 gCAAGCAGGGAGAGTAAATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATcgccgc  >  1:1637489/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                       GTACATGGCGGAAGACCGTCTCGCCTGGTGGGATAAATTCTCCCAGCCGGCCGTGCGCCCCATCTATAGCCTCGGTATCGATACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGAGTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTGCAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGC  >  NC_000913/2274034‑2274300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑