Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,276,298	G→A	*342* (TAG→TAA)	yejE →	microcin C ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,276,298	0	G	A	100.0%	277.8 / NA	84	*342* (TAG→TAA)	yejE	microcin C ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (45/39);  total (45/39)

AGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAG  >  NC_000913/2276169‑2276432                                                                                                                                  |                                                                                                                                         aGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                                 <  2:1316865/139‑1 (MQ=255)   ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                               >  2:1113659/1‑139 (MQ=255)   ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                               >  1:449125/1‑139 (MQ=255)   ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                               >  1:1564820/1‑139 (MQ=255)      aaaaTAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAActc                                                                                                                            >  1:726514/1‑139 (MQ=255)             ccTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑Gc                                                                                                                   <  2:1295855/139‑1 (MQ=255)                 cAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGAtt                                                                                                               >  1:1001766/1‑139 (MQ=255)                 cAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGAtt                                                                                                               >  2:1768195/1‑139 (MQ=255)                        cGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAAtt                                                                                                        >  1:63013/1‑139 (MQ=255)                                      aCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGtttt                                                                                          >  1:1854672/1‑139 (MQ=255)                                                     gTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                           <  2:449125/139‑1 (MQ=255)                                                     gTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                           <  2:1854672/139‑1 (MQ=255)                                                       ggCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                         <  2:1564820/139‑1 (MQ=255)                                                         cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtac                                                                       <  2:726514/139‑1 (MQ=255)                                                          gATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacg                                                                      <  2:63013/139‑1 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAAt                                                                                                         >  1:1738492/1‑92 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAAt                                                                                                         <  2:1738492/92‑1 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                            <  2:1425620/121‑1 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                            >  1:1425620/1‑121 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCaa                                                          >  1:801818/1‑139 (MQ=255)                                                                      tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCaa                                                          >  1:1501177/1‑139 (MQ=255)                                                                       ttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCgg                                                                                                  >  1:487726/1‑98 (MQ=255)                                                                       ttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCgg                                                                                                  <  2:487726/98‑1 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCa                                                                                     <  2:1399518/110‑1 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCa                                                                                     >  1:1399518/1‑110 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatg                                                        >  1:973896/1‑139 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatg                                                        >  2:1297026/1‑139 (MQ=255)                                                                        tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatg                                                        >  1:268428/1‑139 (MQ=255)                                                                         gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                               >  2:670237/1‑69 (MQ=255)                                                                         gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCaaa                                                                                                                               <  1:670237/69‑1 (MQ=255)                                                                         gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       >  2:824596/1‑139 (MQ=255)                                                                         gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       >  2:1540290/1‑139 (MQ=255)                                                                         gCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       >  1:1606949/1‑139 (MQ=255)                                                                           tGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCAGCAGCAAACCGTACGTACAGTAGTCAatgatg                                                     <  1:1113659/139‑1 (MQ=255)                                                                              tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGttt                                                  >  2:36054/1‑139 (MQ=255)                                                                              tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGttt                                                  >  1:143601/1‑139 (MQ=255)                                                                               cTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTc                                                 <  1:36054/139‑1 (MQ=255)                                                                               cTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTc                                                 <  1:1297026/139‑1 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGTGGTGTAACATGACGCa                                                                                                                                 <  2:1384487/58‑1 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGTGGTGTAACATGACGCa                                                                                                                                 >  1:1384487/1‑58 (MQ=255)                                                                                  tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACt                                              >  2:1479778/1‑139 (MQ=255)                                                                                   aTTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                             <  2:1001766/139‑1 (MQ=255)                                                                                   aTTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                             <  2:143601/139‑1 (MQ=255)                                                                                       gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                                 <  2:337209/53‑1 (MQ=255)                                                                                       gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCa                                                                                                                                 >  1:337209/1‑53 (MQ=255)                                                                                       gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                    <  2:938375/112‑1 (MQ=255)                                                                                       gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                    >  1:938375/1‑112 (MQ=255)                                                                                         gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAtt                                       >  1:1099471/1‑139 (MQ=255)                                                                                          aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                              <  2:1293453/99‑1 (MQ=255)                                                                                          aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                              >  1:1293453/1‑99 (MQ=255)                                                                                            gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAg                                    >  1:1767109/1‑139 (MQ=255)                                                                                                 ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCt                                 >  2:1529685/1‑139 (MQ=255)                                                                                                 ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAGTTTGTCGGTGGGTTTTCGcc                                                                                      >  1:1223897/1‑84 (MQ=255)                                                                                                 ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGcc                                                                                      <  2:1223897/84‑1 (MQ=255)                                                                                                 ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                    >  2:4020/1‑102 (MQ=255)                                                                                                 ccGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                    <  1:4020/102‑1 (MQ=255)                                                                                                             tGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggc                   <  1:1479778/139‑1 (MQ=255)                                                                                                              gATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggcg                  <  1:1540290/139‑1 (MQ=255)                                                                                                              gATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggcg                  <  1:824596/139‑1 (MQ=255)                                                                                                               aTCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggcgc                 <  2:268428/139‑1 (MQ=255)                                                                                                                 ccTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                       >  2:1133589/1‑67 (MQ=255)                                                                                                                 ccTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                       <  1:1133589/67‑1 (MQ=255)                                                                                                                 ccTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       <  1:1705656/99‑1 (MQ=255)                                                                                                                 ccTAATAAGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       >  2:1705656/1‑99 (MQ=255)                                                                                                                    aataaGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtg            >  2:475989/1‑139 (MQ=255)                                                                                                                     ataaGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg           <  1:633203/139‑1 (MQ=255)                                                                                                                     ataaGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg           <  1:1524226/139‑1 (MQ=255)                                                                                                                     ataaGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg           <  2:801818/139‑1 (MQ=255)                                                                                                                      taaGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg          <  1:609355/139‑1 (MQ=255)                                                                                                                      taaGGCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg          <  2:1606949/139‑1 (MQ=255)                                                                                                                         ggCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                           >  2:1359372/1‑71 (MQ=255)                                                                                                                         ggCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                           <  1:1359372/71‑1 (MQ=255)                                                                                                                         ggCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       >  1:1024025/1‑91 (MQ=255)                                                                                                                         ggCGGTGTAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                       <  2:1024025/91‑1 (MQ=255)                                                                                                                             gtgtAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                              >  2:1344805/1‑64 (MQ=255)                                                                                                                             gtgtAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                              <  1:1344805/64‑1 (MQ=255)                                                                                                                             gtgtAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCa   >  2:917428/1‑139 (MQ=255)                                                                                                                              tgtAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAg  >  2:908729/1‑139 (MQ=255)                                                                                                                              tgtAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAg  >  1:1744387/1‑139 (MQ=255)                                                                                                                              tgtAACATGACACAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                            <  1:1326909/65‑1 (MQ=255)                                                                                                                              tgtAACATGACACAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                            >  2:1326909/1‑65 (MQ=255)                                                                                                                               gtAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGttt                                                                                           >  2:1877327/1‑49 (MQ=255)                                                                                                                               gtAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAtt                                       >  2:1035431/1‑101 (MQ=255)                                                                                                                               gtAACATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAtt                                       <  1:1035431/101‑1 (MQ=255)                                                                                                                                  |                                                                                                                                         AGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTA‑‑GCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAG  >  NC_000913/2276169‑2276432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑