Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,973	T→C	G146G (GGA→GGG)	ccmH ←	heme lyase, CcmH subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,973	0	T	C	100.0%	178.1 / NA	50	G146G (GGA→GGG)	ccmH	heme lyase, CcmH subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

GCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCAC  >  NC_000913/2291839‑2292107                                                                                                                                       |                                                                                                                                       gcCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCgg                                                                                                                                    <  2:1419678/139‑1 (MQ=255)     tGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACg                                                                                                                                >  2:438192/1‑139 (MQ=255)                 ccGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATTATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCgg                                                                                                                    >  1:1165309/1‑139 (MQ=255)                 ccGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCtt                                                                                                                             <  1:65570/130‑1 (MQ=255)                 ccGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCtt                                                                                                                             >  2:65570/1‑130 (MQ=255)                 ccGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCgg                                                                                                                    >  2:1274669/1‑139 (MQ=255)                  cGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATGAACAACATACCCGGCACGCTTACCTTCCGGc                                                                                                                   <  1:819420/139‑1 (MQ=255)                  cGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGc                                                                                                                   <  2:1380043/139‑1 (MQ=255)                  cGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGc                                                                                                                   <  1:605971/139‑1 (MQ=255)                       ccTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              <  1:438192/139‑1 (MQ=255)                           tgCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGt                                                                                                          <  2:1727735/139‑1 (MQ=255)                           tgCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGt                                                                                                          <  2:1542839/139‑1 (MQ=255)                            gCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGtt                                                                                                         <  1:790707/139‑1 (MQ=255)                                               ttttCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcac                                                                                      <  2:1848857/139‑1 (MQ=255)                                               ttttCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcac                                                                                      <  1:1274669/139‑1 (MQ=255)                                                                   ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              >  2:438212/1‑95 (MQ=255)                                                                   ccAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              <  1:438212/95‑1 (MQ=255)                                                                     aGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAg                                                                <  2:473897/139‑1 (MQ=255)                                                                      gTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCTGTCACGCTTACCTTCCGGCACGCTTTg                                                                                                           >  1:1096487/1‑95 (MQ=255)                                                                      gTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              <  2:395047/92‑1 (MQ=255)                                                                      gTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              >  1:395047/1‑92 (MQ=255)                                                                      gTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTg                                                                                                           <  2:1096487/95‑1 (MQ=255)                                                                       tCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCg                                                                                                                                     <  1:1032864/68‑1 (MQ=255)                                                                       tCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCg                                                                                                                                     >  2:1032864/1‑68 (MQ=255)                                                                                 gCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATg                                                    >  2:511681/1‑139 (MQ=255)                                                                                    gACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              >  1:1652832/1‑78 (MQ=255)                                                                                    gACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              <  2:1652832/78‑1 (MQ=255)                                                                                     aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGc                                                                                                                                   <  1:256293/56‑1 (MQ=255)                                                                                     aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGc                                                                                                                                   >  2:256293/1‑56 (MQ=255)                                                                                     aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAccc                                                >  2:1402501/1‑139 (MQ=255)                                                                                     aCGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAccc                                                >  1:204529/1‑139 (MQ=255)                                                                                           gccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCtt                                                                                             >  1:378569/1‑88 (MQ=255)                                                                                           gccaCAATTAACGCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCtt                                                                                             <  2:378569/88‑1 (MQ=255)                                                                                                       gCCACCACAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAg                              >  2:1618596/1‑139 (MQ=255)                                                                                                          accacAATACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCa                           >  1:422730/1‑139 (MQ=255)                                                                                                                 tACCCGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTgg                    >  2:844429/1‑139 (MQ=255)                                                                                                                   cccGGCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGgca                  <  2:204529/139‑1 (MQ=255)                                                                                                                      ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcacc                                                                                     <  1:564223/69‑1 (MQ=255)                                                                                                                      ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGcacc                                                                                     >  2:564223/1‑69 (MQ=255)                                                                                                                      ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGgcagca               >  1:1041050/1‑139 (MQ=255)                                                                                                                      ggCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGgcagca               >  2:590778/1‑139 (MQ=255)                                                                                                                       gCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              >  2:1392134/1‑43 (MQ=255)                                                                                                                       gCAGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCt                                                                                                              <  1:1392134/43‑1 (MQ=255)                                                                                                                         aGATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCAccc            >  1:293590/1‑139 (MQ=255)                                                                                                                          gATAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCca           <  2:422730/139‑1 (MQ=255)                                                                                                                           aTAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAAc                                                                                                 >  2:1091568/1‑52 (MQ=255)                                                                                                                           aTAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTCGTTCAGTAAAc                                                                                                 <  1:1091568/52‑1 (MQ=255)                                                                                                                            tAAACAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCcaca         <  1:844429/139‑1 (MQ=255)                                                                                                                             aaaCAACATACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCAcag        >  1:995788/1‑139 (MQ=255)                                                                                                                                   caTACCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCAc  <  2:995788/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCAC  >  NC_000913/2291839‑2292107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑