Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 220,239 C→T L230L (CTG→CTA)  metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913220,2390CT100.0% 206.8 / NA 64L230L (CTG→CTA) metQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/31);  total (33/31)

GCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTT  >  NC_000913/220104‑220372
                                                                                                                                       |                                                                                                                                     
gCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAgg                                                                                                                                    >  2:358659/1‑139 (MQ=255)
  cTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGtt                                                                                                                                  >  1:550305/1‑139 (MQ=255)
           aCCAGCCTTTACCAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacgta                                                                                                                            <  1:108311/136‑1 (MQ=255)
           aCCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacgta                                                                                                                            >  2:108311/1‑136 (MQ=255)
            ccAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                                        <  1:834980/139‑1 (MQ=255)
               gCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGAc                                                                                                                     >  1:1159539/1‑139 (MQ=255)
                      aCAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTAt                                                                                                              <  2:1159539/139‑1 (MQ=255)
                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                          >  2:343351/1‑139 (MQ=255)
                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                          >  1:868059/1‑139 (MQ=255)
                          cTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                          >  1:448545/1‑139 (MQ=255)
                             cgccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCt                                                                                                            <  1:1599476/134‑1 (MQ=255)
                             cgccgTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCt                                                                                                            >  2:1599476/1‑134 (MQ=255)
                                          tttgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCt                                                                                          >  2:1006685/1‑139 (MQ=255)
                                            tgtttgCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCttt                                                                                        >  2:1839521/1‑139 (MQ=255)
                                                tgctgcTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                                    <  2:377093/139‑1 (MQ=255)
                                                tgctgcTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                                    <  2:550305/139‑1 (MQ=255)
                                                tgctgcTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                                    <  2:448545/139‑1 (MQ=255)
                                                       tCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCa                                                                             >  2:458425/1‑139 (MQ=255)
                                                                 cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg                                                                   <  2:1414058/139‑1 (MQ=255)
                                                                 cGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg                                                                   >  1:1414058/1‑139 (MQ=255)
                                                                          gATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATa                                                          >  2:531886/1‑139 (MQ=255)
                                                                            tAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                        <  1:1451724/139‑1 (MQ=255)
                                                                                  tGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTAt                                                  <  2:493823/139‑1 (MQ=255)
                                                                                    gACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTg                                                >  1:406042/1‑139 (MQ=255)
                                                                                     aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTTAGGCCAATCTGGCTGGCATAGGTGGTATTGa                                               >  2:1454924/1‑139 (MQ=255)
                                                                                     aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                                  >  2:984262/1‑120 (MQ=255)
                                                                                     aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctgg                                                                  <  1:984262/120‑1 (MQ=255)
                                                                                     aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGa                                               >  1:523359/1‑139 (MQ=255)
                                                                                     aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGa                                               >  1:1817820/1‑139 (MQ=255)
                                                                                     aCGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTAGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGa                                               >  1:1538419/1‑139 (MQ=255)
                                                                                         aTTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAAc                                           >  1:1412605/1‑139 (MQ=255)
                                                                                         aTTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAAc                                           <  1:1839521/139‑1 (MQ=255)
                                                                                                 aCGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCagag                                   <  1:256839/139‑1 (MQ=255)
                                                                                                      ctcGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                                        <  2:57507/49‑1 (MQ=255)
                                                                                                      ctcGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACggg                                                                                                                        >  1:57507/1‑49 (MQ=255)
                                                                                                       tcGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacg                                                                                                                              >  1:164072/1‑42 (MQ=255)
                                                                                                       tcGGCGTCTTTGTTATCTTCACGCGTCACGATTAGGTTtacg                                                                                                                              <  2:164072/42‑1 (MQ=255)
                                                                                                            gTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                        <  2:1412605/139‑1 (MQ=255)
                                                                                                            gTCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                        <  2:1722691/139‑1 (MQ=255)
                                                                                                             tCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                             >  1:1015732/1‑101 (MQ=255)
                                                                                                             tCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCtggctggc                                                             <  2:1015732/101‑1 (MQ=255)
                                                                                                             tCTTTGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGcgt                       <  1:1006685/139‑1 (MQ=255)
                                                                                                                 tGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAg                                                                                                   >  1:764319/1‑59 (MQ=255)
                                                                                                                 tGTTATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAg                                                                                                   <  2:764319/59‑1 (MQ=255)
                                                                                                                   ttATCTTCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCa                 <  1:458425/139‑1 (MQ=255)
                                                                                                                        ttCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                        <  2:1381665/127‑1 (MQ=255)
                                                                                                                        ttCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                        >  1:1381665/1‑127 (MQ=255)
                                                                                                                        ttCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGaa            >  2:1358612/1‑139 (MQ=255)
                                                                                                                         tCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGc                                                                                     >  1:800232/1‑65 (MQ=255)
                                                                                                                         tCACGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGc                                                                                     <  2:800232/65‑1 (MQ=255)
                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                        >  2:42667/1‑92 (MQ=255)
                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                        >  2:536077/1‑92 (MQ=255)
                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                        <  1:536077/92‑1 (MQ=255)
                                                                                                                           aCGCGTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAgg                                                        <  1:42667/92‑1 (MQ=255)
                                                                                                                             gcgTCACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCgg       <  2:523359/139‑1 (MQ=255)
                                                                                                                                tCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                          <  2:857719/37‑1 (MQ=255)
                                                                                                                                tCACGATTAGGTTTACGTACGGGGACTCTTTATCTTc                                                                                                          >  1:857719/1‑37 (MQ=255)
                                                                                                                                 cACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                                    <  2:319405/58‑1 (MQ=255)
                                                                                                                                 cACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGcc                                                                                    >  1:319405/1‑58 (MQ=255)
                                                                                                                                 cACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                        >  1:400255/1‑118 (MQ=255)
                                                                                                                                 cACGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGcgcg                        <  2:400255/118‑1 (MQ=255)
                                                                                                                                  aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTTAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGtt  <  1:1454924/139‑1 (MQ=255)
                                                                                                                                  aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg                                                                   >  1:1723479/1‑74 (MQ=255)
                                                                                                                                  aCGATTAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATctg                                                                   <  2:1723479/74‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GCCTAAAAATTACCAGCCTTTAACAGCTCCGCCGTTAAACACTTTGTTTGCTGCTTCGTAAACTTCGTCAGACTGATAAGCCTGGACGAATTTCTTCACGTTCTCGGCGTCTTTGTTATCTTCACGCGTCACGATCAGGTTTACGTACGGGGACTCTTTATCTTCAACAAAGATACCGTCTTTCGCCGGAGTCAGGCCAATCTGGCTGGCATAGGTGGTATTGATAACTGCCAGAGCGATTTGCGCGTCGTCCAGAGAACGCGGCAGTT  >  NC_000913/220104‑220372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑