Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,370,019	T→C	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA → arnD →	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,370,019	0	T	C	100.0%	225.7 / NA	63	*661R (TGA→CGA)  M1T (ATG→ACG) †	arnA arnD	fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase undecaprenyl phosphate‑alpha‑L‑ara4FN deformylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/35);  total (28/35)

TAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAATAT  >  NC_000913/2369885‑2370151                                                                                                                                       |                                                                                                                                     tAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAcc                                                                                                                                  <  2:382329/139‑1 (MQ=255)    agCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCaaa                                                                                                                               >  2:1409047/1‑139 (MQ=255)     gCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                   >  1:765438/1‑134 (MQ=255)     gCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                   <  2:765438/134‑1 (MQ=255)     gCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAg                                                                                                                              <  2:1173702/139‑1 (MQ=255)          gTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                         <  2:1225906/139‑1 (MQ=255)          gTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                         <  1:418684/139‑1 (MQ=255)                  aGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAt                                                                                                                 >  1:1760651/1‑139 (MQ=255)                   gCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTT‑CTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                   >  2:456357/1‑119 (MQ=255)                   gCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTT‑CTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                   <  1:456357/119‑1 (MQ=255)                                   ccGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                                >  2:1805937/1‑123 (MQ=255)                                   ccGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCAtt                                                                                                                <  1:1805937/123‑1 (MQ=255)                                         ccTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCa                                                                                          >  2:776865/1‑139 (MQ=255)                                          cTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCAc                                                                                         <  1:776865/139‑1 (MQ=255)                                          cTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCAc                                                                                         <  1:1409047/139‑1 (MQ=255)                                          cTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCAc                                                                                         <  1:1611215/139‑1 (MQ=255)                                          cTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCAc                                                                                         <  2:457573/139‑1 (MQ=255)                                                 gggAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGaa                                                                                  >  1:396249/1‑139 (MQ=255)                                                  ggAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTa                                                                                                                      <  1:416960/102‑1 (MQ=255)                                                  ggAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTa                                                                                                                      >  2:416960/1‑102 (MQ=255)                                                  ggAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAg                                                                                 >  2:1754101/1‑139 (MQ=255)                                                  ggAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCT‑‑‑TGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCg                                                                              >  2:177941/1‑139 (MQ=255)                                                           aaTTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCcgc                                                                        <  2:1062548/139‑1 (MQ=255)                                                                 tatGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTctgc                                                                  <  1:1754101/139‑1 (MQ=255)                                                                   tGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                         >  1:381936/1‑82 (MQ=255)                                                                   tGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGc                                                                                                                         <  2:381936/82‑1 (MQ=255)                                                                      aGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCcgc                                                                        >  2:1032404/1‑128 (MQ=255)                                                                      aGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCcgc                                                                        <  1:1032404/128‑1 (MQ=255)                                                                      aGGAAACCATCGACGAAACGCTGGATTTCT‑‑‑TGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATc                                                          <  1:177941/139‑1 (MQ=255)                                                                             cATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCa                                                                                                                  <  1:1299918/79‑1 (MQ=255)                                                                             cATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCa                                                                                                                  >  2:1299918/1‑79 (MQ=255)                                                                             cATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCa                                                                                          >  1:1762118/1‑103 (MQ=255)                                                                             cATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCa                                                                                          <  2:1762118/103‑1 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                   <  1:996513/60‑1 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGAc                                                                                                                                   >  2:996513/1‑60 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                           <  2:532392/100‑1 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGc                                                                                           >  1:532392/1‑100 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTTAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                    >  2:184292/1‑139 (MQ=255)                                                                               tCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                    <  1:31297/139‑1 (MQ=255)                                                                                cgacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTTAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTa                                                   <  1:184292/139‑1 (MQ=255)                                                                                 gacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                  <  2:1760651/139‑1 (MQ=255)                                                                                 gacgaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTaa                                                  <  2:1540124/139‑1 (MQ=255)                                                                                    gaAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCa                                               <  1:117570/139‑1 (MQ=255)                                                                                         gCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCataata                                          >  2:34982/1‑139 (MQ=255)                                                                                                  cttcCTGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCa                                 >  2:133618/1‑139 (MQ=255)                                                                                                       tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGAt                                                                                                       >  1:497690/1‑64 (MQ=255)                                                                                                       tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGAt                                                                                                       <  2:497690/64‑1 (MQ=255)                                                                                                       tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                    <  2:245624/115‑1 (MQ=255)                                                                                                       tGCGCACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGt                                                    >  1:245624/1‑115 (MQ=255)                                                                                                         cgcACCGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCAtttt                          >  2:654081/1‑139 (MQ=255)                                                                                                             ccGTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTttcttc                      >  1:1440991/1‑139 (MQ=255)                                                                                                               gTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGa                                                              >  2:976266/1‑97 (MQ=255)                                                                                                               gTTGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGa                                                              <  1:976266/97‑1 (MQ=255)                                                                                                                ttGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGc                   >  2:1794445/1‑139 (MQ=255)                                                                                                                ttGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGc                   >  2:1193129/1‑139 (MQ=255)                                                                                                                ttGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCA‑CCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCg                  <  1:1199830/139‑1 (MQ=255)                                                                                                                 tGATCTTACGGATAAACCATCACGACCAAAGTAGTCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCg                  <  2:854022/139‑1 (MQ=255)                                                                                                                 tGATCTTACGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCg                  <  2:1513112/139‑1 (MQ=255)                                                                                                                      ttATGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGc             <  2:396249/139‑1 (MQ=255)                                                                                                                        aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGa                                                                                                              <  1:25348/40‑1 (MQ=39)                                                                                                                        aCGGATAAACCATCACGACCAAAGTAGGCTTACGCATTGa                                                                                                              >  2:25348/1‑40 (MQ=255)                                                                                                                             tAAACCATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACa      >  2:599102/1‑139 (MQ=255)                                                                                                                                 ccATCACGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAatat  <  1:34982/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     TAGAGCATCGTAAACCGAGCATCCGCAATGCCCACCGCTGCCTGGACTGGGAGCCGAAAATTGATATGCAGGAAACCATCGACGAAACGCTGGATTTCTTCCTGCGCACCGTTGATCTTACGGATAAACCATCATGACCAAAGTAGGCTTACGCATTGATGTCGATACCTTTCGTGGCACCCGTGAAGGCGTGCCGCGTCTGCTGGAAATCTTGAGTAAGCATAATATTCAGGCCAGCATTTTTTTCAGCGTCGGCCCGGACAATAT  >  NC_000913/2369885‑2370151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑