Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,453,265 C→T pseudogene (1737/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family,putative membrane, Not classified, putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,453,2650CT99.1% 343.4 / ‑4.5 106pseudogene (1737/2646 nt)yfcUpseudogene, FimD fimbrial export usher family,putative membrane, Not classified, putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (51/54);  total (51/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01

Only 100 of 106 total aligned reads displayed.

GCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCC  >  NC_000913/2453130‑2453397
                                                                                                                                       |                                                                                                                                    
gCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTAc                                                                                                                                   <  2:1005109/139‑1 (MQ=255)
         cAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTa                                                                                                                          >  2:136831/1‑139 (MQ=255)
               aTAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                    <  2:330805/139‑1 (MQ=255)
               aTAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                    >  2:1868917/1‑139 (MQ=255)
                         gcgTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCAt                                                                                                          >  1:25973/1‑139 (MQ=255)
                         gcgTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCAt                                                                                                          >  1:19813/1‑139 (MQ=255)
                          cgTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATa                                                                                                         >  1:1301791/1‑139 (MQ=255)
                              aTCGACACGGCTAAAATAACCGACCTGGCTGCTGTCGGTCCCACTGCGATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCGCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCg                                                                                                     <  2:955335/139‑1 (MQ=255)
                              aTCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCg                                                                                                     <  2:1438459/139‑1 (MQ=255)
                              aTCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCg                                                                                                     <  1:136831/139‑1 (MQ=255)
                                     cGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGt                                                                                              >  1:1585684/1‑139 (MQ=255)
                                         tAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGcc                                                                                          <  2:1859171/139‑1 (MQ=255)
                                         tAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGcc                                                                                          <  2:25973/139‑1 (MQ=255)
                                                aCCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCAt                                                                                                          <  2:879706/116‑1 (MQ=255)
                                                aCCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCAt                                                                                                          >  1:879706/1‑116 (MQ=255)
                                                 ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTAc                                                                                                                                   <  1:1703405/90‑1 (MQ=255)
                                                 ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTAc                                                                                                                                   >  2:1703405/1‑90 (MQ=255)
                                                 ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCg                                                                                                     >  2:1209319/1‑120 (MQ=255)
                                                 ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCg                                                                                                     <  1:1209319/120‑1 (MQ=255)
                                                 ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAg                                                                                            >  1:1245863/1‑129 (MQ=255)
                                                 ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAggg                                                                                  >  2:810823/1‑139 (MQ=255)
                                                 ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAggg                                                                                  >  2:1207427/1‑139 (MQ=255)
                                                 ccGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTACAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAg                                                                                            <  2:1245863/129‑1 (MQ=255)
                                                  cGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                 <  2:19813/139‑1 (MQ=255)
                                                  cGACCTGGCTGCTGTCCGTCCCACTGCCATAGATGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAACGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAACTGTAGCCAGTCAGGGa                                                                                 >  1:1823980/1‑139 (MQ=255)
                                                    aCCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGaaa                                                                               <  2:1585684/139‑1 (MQ=255)
                                                     ccTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAAc                                                                              >  2:1630599/1‑139 (MQ=255)
                                                      cTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCACCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                 <  1:1360949/135‑1 (MQ=255)
                                                      cTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCACCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                 >  2:1360949/1‑135 (MQ=255)
                                                         gctgctGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTc                                                                          >  1:803377/1‑139 (MQ=255)
                                                         gctgctGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTAC‑‑GCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCat                                                                        >  1:1432881/1‑139 (MQ=255)
                                                         gctgctGTCCGCCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGaaa                                                                               <  2:123990/134‑1 (MQ=255)
                                                         gctgctGTCCGCCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGaaa                                                                               >  1:123990/1‑134 (MQ=255)
                                                           tgctgTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCat                                                                        <  1:1868917/139‑1 (MQ=255)
                                                             ctgTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGccc                                                                                                                   <  2:1560131/94‑1 (MQ=255)
                                                               gtccgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTa                                                                    >  1:1598622/1‑139 (MQ=255)
                                                                tccgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTAc                                                                   <  2:1880815/139‑1 (MQ=255)
                                                                tccgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTAc                                                                   >  2:1754325/1‑139 (MQ=255)
                                                                  cgtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTAC‑‑GCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAAt                                                               <  2:1432881/139‑1 (MQ=255)
                                                                   gtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                    <  1:667571/87‑1 (MQ=255)
                                                                   gtccCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGcc                                                                                                                    >  2:667571/1‑87 (MQ=255)
                                                                     cccACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCGCACGGCATACTGAGCGAGATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGTAtg                                                              <  1:1207427/139‑1 (MQ=255)
                                                                       cACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                           <  2:322999/124‑1 (MQ=255)
                                                                       cACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                           >  1:322999/1‑124 (MQ=255)
                                                                          tGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                         <  1:810823/139‑1 (MQ=255)
                                                                                aGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATAtt                                                   <  1:1583164/139‑1 (MQ=255)
                                                                                    gCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGt                                                                                                             <  1:1319407/77‑1 (MQ=255)
                                                                                    gCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGt                                                                                                             >  2:1319407/1‑77 (MQ=255)
                                                                                     ccGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCAt                                                                                                          >  1:1498946/1‑79 (MQ=255)
                                                                                     ccGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCAt                                                                                                          <  2:1498946/79‑1 (MQ=255)
                                                                                      cGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGaaa                                               >  1:820021/1‑137 (MQ=255)
                                                                                      cGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGaaa                                               <  2:820021/137‑1 (MQ=255)
                                                                                          ataGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTgg                                         <  1:1754325/139‑1 (MQ=255)
                                                                                            aGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGga                                       <  1:1630599/139‑1 (MQ=255)
                                                                                              cTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGc                                                                                           <  2:1053530/85‑1 (MQ=255)
                                                                                              cTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGc                                                                                           >  1:1053530/1‑85 (MQ=255)
                                                                                                aaCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGt                                                                                                                <  1:988477/62‑1 (MQ=255)
                                                                                                aaCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCatat                                                    <  2:1777608/122‑1 (MQ=255)
                                                                                                aaCGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCatat                                                    >  1:1777608/1‑122 (MQ=255)
                                                                                                  cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCg                                                                                                     >  2:789171/1‑71 (MQ=255)
                                                                                                  cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCg                                                                                                     <  1:789171/71‑1 (MQ=255)
                                                                                                  cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAAtgct                                                            <  1:1062283/112‑1 (MQ=255)
                                                                                                  cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                         <  2:1470616/115‑1 (MQ=255)
                                                                                                  cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                         >  1:1470616/1‑115 (MQ=255)
                                                                                                  cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCa                                                       >  2:929819/1‑117 (MQ=255)
                                                                                                  cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCa                                                       <  1:929819/117‑1 (MQ=255)
                                                                                                  cGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCat                                 >  2:399568/1‑139 (MQ=255)
                                                                                                        tgttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCg                                                                                                                      >  2:165290/1‑48 (MQ=255)
                                                                                                        tgttgtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCg                                                                                                                      <  1:165290/48‑1 (MQ=255)
                                                                                                          ttgtCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGAtgttgt                         <  2:1648175/139‑1 (MQ=255)
                                                                                                            gtCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAg                       <  2:847339/139‑1 (MQ=255)
                                                                                                             tCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCg                                                                                                                      <  2:1304943/43‑1 (MQ=255)
                                                                                                             tCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCg                                                                                                                      >  1:1304943/1‑43 (MQ=255)
                                                                                                             tCGCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCt                                                                           >  2:267293/1‑86 (MQ=255)
                                                                                                               gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCgg                                                                                                                 <  2:694885/46‑1 (MQ=255)
                                                                                                               gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCgg                                                                                                                 >  1:694885/1‑46 (MQ=255)
                                                                                                               gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGc                                                                                           >  1:516096/1‑68 (MQ=255)
                                                                                                               gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGc                                                                                           <  2:516096/68‑1 (MQ=255)
                                                                                                               gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAAtgctg                                                           >  1:156637/1‑100 (MQ=255)
                                                                                                               gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGttt                    >  2:1677059/1‑139 (MQ=255)
                                                                                                               gCCCCACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGttt                    >  2:1660263/1‑139 (MQ=255)
                                                                                                                  ccACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                 >  1:1840303/1‑75 (MQ=255)
                                                                                                                  ccACGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGa                                                                                 <  2:1840303/75‑1 (MQ=255)
                                                                                                                    aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTc                                                                                                            >  1:273418/1‑46 (MQ=255)
                                                                                                                    aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                         >  2:1353723/1‑97 (MQ=255)
                                                                                                                    aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGcc                                                         <  1:1353723/97‑1 (MQ=255)
                                                                                                                    aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGga                                       <  1:1735403/115‑1 (MQ=255)
                                                                                                                    aCGGCATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGga                                       >  2:1735403/1‑115 (MQ=255)
                                                                                                                        cATACTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGctcc           >  2:1095980/1‑139 (MQ=255)
                                                                                                                           aCTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAAt                                              >  2:1488432/1‑101 (MQ=255)
                                                                                                                           aCTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAAt                                              <  1:1488432/101‑1 (MQ=255)
                                                                                                                           aCTGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCGCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCa        <  1:1095980/139‑1 (MQ=255)
                                                                                                                           aCTGAGCGAAATTTACATGCCCTTATCCGCCCGGGTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCa        <  2:803377/139‑1 (MQ=255)
                                                                                                                             tGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCa                                  <  1:275678/111‑1 (MQ=255)
                                                                                                                             tGAGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAACAGTGGGAGAGCa                                  >  2:275678/1‑111 (MQ=255)
                                                                                                                               aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGAt    <  1:1585413/139‑1 (MQ=255)
                                                                                                                               aGCGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGAt    <  2:1301791/139‑1 (MQ=255)
                                                                                                                                 cGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGc                                                                                           >  2:698365/1‑50 (MQ=255)
                                                                                                                                 cGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGc                                                                                           <  1:698365/50‑1 (MQ=255)
                                                                                                                                 cGAAATTTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATcc  >  1:201534/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                    
GCCGATGTTCAACTGATAGTGAGTCGCGTCATCGACACGGCTAAAATAACCGACCTGGCTGCTGTCCGTCCCACTGCCATAGTTGCCGTTATAGCTAACGGTACTGTTGTCGCCCCACGGCATACTGAGCGAAATCTACATGCCCTTATCCGCCCGGTTGTCATACTCGTAGCGGTAGCCAGTCAGGGAAACGCTCATATTACGAATGCTGCCCATATTGAAATAGTGGGAGAGCATGATGTTGTAGTTTGTCTGCTCCTCACGATCC  >  NC_000913/2453130‑2453397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: