Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	247,461	G→A	*250* (TAG→TAA)	yafL →	putative lipoprotein and C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	247,461	0	G	A	100.0%	150.1 / NA	47	*250* (TAG→TAA)	yafL	putative lipoprotein and C40 family peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/24);  total (23/24)

TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NC_000913/247325‑247594                                                                                                                                         |                                                                                                                                      tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGa                                                                                                                                     >  2:557170/1‑139 (MQ=255) tgtATTTGGGCGATGGACAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGACTAGCCGACCCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGa                                                                                                                                     >  1:292376/1‑139 (MQ=255)              tGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGttt                                                                                                                       >  1:572114/1‑139 (MQ=255)                           gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCgg                                                                                                          <  1:728678/139‑1 (MQ=255)                               cGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGt                                                                                                      >  1:542048/1‑139 (MQ=255)                                gCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGtt                                                                                                     <  2:1419296/139‑1 (MQ=255)                                   aCGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                  <  1:557170/139‑1 (MQ=255)                                       aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                              >  1:1760629/1‑139 (MQ=255)                                          ggCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGt                                                                                           <  1:1798541/139‑1 (MQ=255)                                               aaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTg                                                                                      >  1:658353/1‑139 (MQ=255)                                                   aTTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGc                                                                                  >  2:1050769/1‑139 (MQ=255)                                                     tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgc                                                                                                                   >  2:1445668/1‑104 (MQ=255)                                                     tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTAcgc                                                                                                                   <  1:1445668/104‑1 (MQ=255)                                                            aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:944643/139‑1 (MQ=255)                                                                  ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                   >  2:1368420/1‑139 (MQ=255)                                                                    aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                 <  2:542048/139‑1 (MQ=255)                                                                              ttCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTAc                                                       >  1:410403/1‑139 (MQ=255)                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                        <  2:1104253/105‑1 (MQ=255)                                                                               tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                      >  1:369113/1‑139 (MQ=255)                                                                               tCTGGCAGGACCATTTTTTGCGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                        >  1:1104253/1‑105 (MQ=38)                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGTAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                                                        <  1:1807099/120‑1 (MQ=255)                                                                                cTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGTAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgcg                                                                        >  2:1807099/1‑120 (MQ=38)                                                                                 tGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                    <  2:1760629/139‑1 (MQ=255)                                                                                   gCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGcc                                                  >  1:1301395/1‑139 (MQ=255)                                                                                    cAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGccc                                                 <  1:1368420/139‑1 (MQ=255)                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                  >  1:941398/1‑86 (MQ=38)                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTa                                                                                                  <  2:941398/86‑1 (MQ=255)                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         >  1:1689317/1‑111 (MQ=255)                                                                                        aCCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  2:1689317/111‑1 (MQ=255)                                                                                                    gcgcgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGc                                 >  1:1039536/1‑139 (MQ=255)                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         <  1:473128/96‑1 (MQ=255)                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                         >  2:473128/1‑96 (MQ=255)                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                    >  1:1476927/1‑117 (MQ=255)                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAg                                                    <  2:1476927/117‑1 (MQ=255)                                                                                                       cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                              >  2:1703692/1‑139 (MQ=255)                                                                                                        gcgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCt                             <  1:1050769/139‑1 (MQ=255)                                                                                                         cgcAGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCtt                            >  1:93634/1‑139 (MQ=255)                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:1039536/139‑1 (MQ=255)                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:410403/139‑1 (MQ=255)                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  1:1703692/139‑1 (MQ=255)                                                                                                            aGGATTTTGACGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:99477/139‑1 (MQ=255)                                                                                                                      cGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATAttt                                       >  1:963907/1‑115 (MQ=255)                                                                                                                      cGGAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATAttt                                       <  2:963907/115‑1 (MQ=255)                                                                                                                       ggAAGAGACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaac              <  2:372275/139‑1 (MQ=255)                                                                                                                          agagACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         <  2:23978/125‑1 (MQ=255)                                                                                                                          agagACGATTTTGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                         >  1:23978/1‑125 (MQ=255)                                                                                                                                   tttGTAAGAC‑GATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc  <  1:874737/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCC  >  NC_000913/247325‑247594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑