Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,511,468 C→T *413* (TAG→TAA mntH ← manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,511,4680CT98.4% 203.8 / ‑5.4 63*413* (TAG→TAAmntHmanganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (29/33);  minor base A (1/0);  total (30/33)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATC  >  NC_000913/2511338‑2511603
                                                                                                                                  |                                                                                                                                       
cAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGGATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATcc                                                                                                                                 <  1:1333845/139‑1 (MQ=255)
cAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATcc                                                                                                                                 >  2:408196/1‑139 (MQ=255)
  aTGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCa                                                                                                                               <  1:153079/139‑1 (MQ=255)
    ggAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAgc                                                                                                                             <  2:730865/139‑1 (MQ=255)
      aGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAgcgc                                                                                                                           >  1:521100/1‑139 (MQ=255)
       gCACAATGCCTGATGCGACGCTAGTGCGTCTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCg                                                                                                                         <  2:592081/139‑1 (MQ=255)
             tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTcccc                                                                                                                    >  1:1373515/1‑139 (MQ=255)
                    tGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                             <  1:853798/139‑1 (MQ=255)
                     gcgcCGCTAGTGCGTCTTATAAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                                      >  2:1279138/1‑113 (MQ=255)
                     gCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                                      <  1:1279138/113‑1 (MQ=255)
                         cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAg                                                                                                        >  2:497599/1‑139 (MQ=255)
                              gTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAtt                                                                                                   >  1:1633118/1‑139 (MQ=255)
                                gCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCa                                                                                                 <  2:1317724/139‑1 (MQ=255)
                                   tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAgcg                                                                                              >  2:851649/1‑139 (MQ=255)
                                      tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCa                                                                                           <  1:497599/139‑1 (MQ=255)
                                        tCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                                                         <  2:1633118/139‑1 (MQ=255)
                                          aGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAc                                                                                       <  1:851649/139‑1 (MQ=255)
                                           ggCCTACAAACCGGCCCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAAATACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAcc                                                                                      >  1:1256110/1‑139 (MQ=255)
                                            gCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                                      >  1:1590386/1‑90 (MQ=255)
                                            gCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTAc                                                                                                                                      <  2:1590386/90‑1 (MQ=255)
                                             ccTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAg                                                                                    <  1:242700/139‑1 (MQ=255)
                                                 cAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcacc                                                                                >  2:1191589/1‑139 (MQ=255)
                                                                aCGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCct                                                                 <  2:685152/139‑1 (MQ=255)
                                                                 cgcgTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctg                                                                <  2:1373515/139‑1 (MQ=255)
                                                                      aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCg                                                                                                                         >  1:1145668/1‑77 (MQ=255)
                                                                      aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCccacc                                                                                                                 >  1:628627/1‑85 (MQ=255)
                                                                      aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCccacc                                                                                                                 <  2:628627/85‑1 (MQ=255)
                                                                      aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt                                                           >  1:924710/1‑139 (MQ=255)
                                                                      aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtctgt                                                           >  1:1186557/1‑139 (MQ=255)
                                                                      aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctg                                                                <  1:587773/134‑1 (MQ=255)
                                                                      aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctg                                                                >  2:587773/1‑134 (MQ=255)
                                                                      aGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATACTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCg                                                                                                                         <  2:1145668/77‑1 (MQ=255)
                                                                          cGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACATTCCCAGCGCCg                                                                                                                         >  2:1607479/1‑73 (MQ=255)
                                                                          cGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACATTCCCAGCGCCg                                                                                                                         <  1:1607479/73‑1 (MQ=255)
                                                                            gATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                             <  2:299114/83‑1 (MQ=255)
                                                                            gATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                             >  1:299114/1‑83 (MQ=255)
                                                                             aTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTAcgc                                                    >  1:275839/1‑139 (MQ=255)
                                                                                  gCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTg                                               <  2:275839/139‑1 (MQ=255)
                                                                                    gTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                             <  1:408196/139‑1 (MQ=255)
                                                                                       tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaa                                                                                                               <  1:823311/70‑1 (MQ=255)
                                                                                       tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaa                                                                                                               <  1:251088/70‑1 (MQ=255)
                                                                                       tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaa                                                                                                               >  2:251088/1‑70 (MQ=255)
                                                                                       tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaa                                                                                                               >  2:823311/1‑70 (MQ=255)
                                                                                       tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAcc                                                                                      >  2:13424/1‑95 (MQ=255)
                                                                                       tACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGAcc                                                                                      <  1:13424/95‑1 (MQ=255)
                                                                                       tACGCCGCACCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCccac                                                                                                                  <  2:1464919/67‑1 (MQ=255)
                                                                                       tACGCCGCACCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCccac                                                                                                                  >  1:1464919/1‑67 (MQ=255)
                                                                                             gcATCCGCCAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAg                                    >  2:452566/1‑139 (MQ=255)
                                                                                                     cAATAGTGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCa                            <  1:452566/139‑1 (MQ=255)
                                                                                                           tGCCAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACt                      >  2:336088/1‑139 (MQ=255)
                                                                                                             ccAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                                                         <  2:977563/70‑1 (MQ=255)
                                                                                                             ccAGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACg                                                                                         >  1:977563/1‑70 (MQ=255)
                                                                                                               aGATGCGACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCt                  <  2:1186557/139‑1 (MQ=255)
                                                                                                                     gACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                             >  2:1494561/1‑42 (MQ=255)
                                                                                                                     gACGCTCATTCAATTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                             <  1:1494561/42‑1 (MQ=255)
                                                                                                                      aCGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgt                                                               >  1:943249/1‑87 (MQ=255)
                                                                                                                      aCGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgt                                                               <  2:943249/87‑1 (MQ=255)
                                                                                                                       cGCTCATTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTgg          <  1:1191589/139‑1 (MQ=255)
                                                                                                                            aTTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCaa                        >  2:1818724/1‑120 (MQ=255)
                                                                                                                            aTTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCaa                        <  1:1818724/120‑1 (MQ=255)
                                                                                                                            aTTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGaaa     >  2:21804/1‑139 (MQ=255)
                                                                                                                            aTTCAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGaaa     >  2:902993/1‑139 (MQ=255)
                                                                                                                               cAATTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACATTCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGGCCATCAACTTGCTGTCACTGGTGAAAATc  <  2:1256110/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                       
CAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATC  >  NC_000913/2511338‑2511603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: