Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,525,930 C→T *309* (TAG→TAA yfeR ← transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9300CT100.0% 169.0 / NA 52*309* (TAG→TAAyfeRtranscriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/23);  total (29/23)

ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCAT  >  NC_000913/2525794‑2526052
                                                                                                                                        |                                                                                                                          
caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta                                                                                                                          >  1:1451286‑M2/133‑139 (MQ=255)
  acatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTtata                                                                                                                        <  1:772247‑M2/9‑1 (MQ=255)
                  cggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATc                                                                                                        <  2:945815‑M2/25‑1 (MQ=255)
                   ggggattcagtatcgataggcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGAATcc                                                                                                       >  2:1238925‑M2/114‑139 (MQ=255)
                     ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc                                                                                                     >  1:1440906‑M2/112‑139 (MQ=255)
                     ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc                                                                                                     >  1:1737618‑M2/112‑139 (MQ=255)
                     ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc                                                                                                     >  1:1727300‑M2/112‑139 (MQ=255)
                       attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTatat                                                                                                                       <  2:987158‑M2/10‑1 (MQ=255)
                       attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTatat                                                                                                                       >  1:987158‑M2/110‑119 (MQ=255)
                       attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt                                                                                                   >  1:544043‑M2/110‑139 (MQ=255)
                         tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGa                                                                                                                   >  2:1052238‑M2/108‑121 (MQ=255)
                         tcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGa                                                                                                                   <  1:1052238‑M2/14‑1 (MQ=255)
                                       cggatgacaaactgggcgaatatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg                                                                                   >  1:1106003‑M2/94‑139 (MQ=255)
                                       cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                                  >  1:645851‑M2/94‑124 (MQ=255)
                                       cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc                                                                                                  <  2:645851‑M2/31‑1 (MQ=255)
                                         gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACaa                                                                                                              <  1:1787608‑M2/19‑1 (MQ=255)
                                         gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACaa                                                                                                              >  2:1787608‑M2/92‑110 (MQ=255)
                                           tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                               <  2:1451286‑M2/50‑1 (MQ=255)
                                           tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                               <  1:665541‑M2/50‑1 (MQ=255)
                                           tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                               <  2:1737618‑M2/50‑1 (MQ=255)
                                                       cgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtct                                                                   >  2:1255507‑M2/78‑139 (MQ=255)
                                                             tttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                             <  2:544043‑M2/68‑1 (MQ=255)
                                                             tttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                             <  1:941259‑M2/68‑1 (MQ=255)
                                                                ttaccaggatgcgataatctattccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg                                                          <  2:1106003‑M2/71‑1 (MQ=255)
                                                                           cgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGc                                               >  1:1561832‑M2/58‑139 (MQ=255)
                                                                                atctactccatcaaatttaacttctgattgattactgccggggctcccggctCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGCCCCATAAAGCTTCAg                                          <  1:1255507‑M2/87‑1 (MQ=255)
                                                                                 tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgc                                         >  2:1781650‑M2/52‑139 (MQ=255)
                                                                                        catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                                  >  1:667745‑M2/45‑139 (MQ=255)
                                                                                        catcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTg                                  >  2:576469‑M2/45‑139 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc                                                                                              <  2:703192‑M2/35‑1 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGc                                                                                              >  1:703192‑M2/43‑77 (MQ=255)
                                                                                          tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                >  1:1834568‑M2/43‑139 (MQ=255)
                                                                                           caaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                >  1:1869508‑M2/42‑58 (MQ=255)
                                                                                           caaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                <  2:1869508‑M2/17‑1 (MQ=255)
                                                                                             aatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAg                             >  2:360177‑M2/40‑139 (MQ=255)
                                                                                               tttaacttctgattgattactgccggggctcccgacaCACTTTATATCTGATACAACgg                                                                                                           <  2:661886‑M2/22‑1 (MQ=255)
                                                                                               tttaacttctgattgattactgccggggctcccgacaCACTTTATATCTGATACAACgg                                                                                                           >  1:661886‑M2/38‑59 (MQ=255)
                                                                                                  aacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                         >  2:1381738‑M2/35‑90 (MQ=255)
                                                                                                  aacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                         <  1:1381738‑M2/56‑1 (MQ=255)
                                                                                                    cttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                >  1:117747‑M2/33‑49 (MQ=255)
                                                                                                    cttctgattgattactgccggggctcccggcaCACTTTATATCTGATAc                                                                                                                <  2:117747‑M2/17‑1 (MQ=255)
                                                                                                          attgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                >  2:566155‑M2/27‑139 (MQ=255)
                                                                                                           ttgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTcc               <  2:1004716‑M2/114‑1 (MQ=255)
                                                                                                              attactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgc            >  1:1541587‑M2/23‑139 (MQ=255)
                                                                                                                 actgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa         <  2:1440906‑M2/120‑1 (MQ=255)
                                                                                                                  ctgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCAGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAc        <  2:667745‑M2/121‑1 (MQ=255)
                                                                                                                   tgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAcc       >  2:1352620‑M2/18‑139 (MQ=255)
                                                                                                                        gggctcccggcaCACTTTATATCTGATACAACGGATcc                                                                                                       >  2:1291183‑M2/13‑38 (MQ=255)
                                                                                                                        gggctcccggcaCACTTTATATCTGATACAACGGATcc                                                                                                       <  1:1291183‑M2/26‑1 (MQ=255)
                                                                                                                        gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                       <  2:110394‑M2/58‑1 (MQ=255)
                                                                                                                        gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg                                                                       >  1:110394‑M2/13‑70 (MQ=255)
                                                                                                                        gggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCAt  >  2:64518‑M2/13‑139 (MQ=255)
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ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCAT  >  NC_000913/2525794‑2526052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: