Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,533,370	T→C	L321S (TTG→TCG)	cysK →	cysteine synthase A, O‑acetylserine sulfhydrolase A subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,533,370	0	T	C	100.0%	314.2 / NA	87	L321S (TTG→TCG)	cysK	cysteine synthase A, O‑acetylserine sulfhydrolase A subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/44);  total (43/44)

CAGCTGTTGCCGCGGCGTTGAAACTACAAGAAGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAA  >  NC_000913/2533235‑2533502                                                                                                                                        |                                                                                                                                     cagcTGTTGCCGCGGCGTTGAAACTACAAGAAGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCa                                                                                                                                   <  1:1556373/139‑1 (MQ=255)         gCCGCGGCGTTGAAACTACAAGAAGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAAt                                                                                                                           >  2:1152419/1‑139 (MQ=255)              ggcgTTGAAACTACAAGAAGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAg                                                                                                                      <  1:1152419/139‑1 (MQ=255)                          aCAAGAAGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCa                                                                                                                                   >  1:714359/1‑114 (MQ=255)                          aCAAGAAGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCa                                                                                                                                   <  2:714359/114‑1 (MQ=255)                                   tGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCa                                                                                                                                   <  2:994212/105‑1 (MQ=255)                                   tGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCa                                                                                                                                   >  1:994212/1‑105 (MQ=255)                                              ccAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCttttt                                                                                      <  1:1576449/139‑1 (MQ=255)                                                             tgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTtgtg                                                                       >  1:1700193/1‑139 (MQ=255)                                                             tgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTtgtg                                                                       >  2:396595/1‑139 (MQ=255)                                                             tgGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTtgtg                                                                       >  1:1532245/1‑139 (MQ=255)                                                                   ttCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGc                                                                                              <  2:806889/110‑1 (MQ=255)                                                                   ttCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGc                                                                                              >  1:806889/1‑110 (MQ=255)                                                                       aCCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCa                                                             <  1:1451729/139‑1 (MQ=255)                                                                       aCCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCa                                                             >  2:270993/1‑139 (MQ=255)                                                                         catcatCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGcc                                                                                                                        <  2:1263363/78‑1 (MQ=255)                                                                         catcatCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGcc                                                                                                                        >  1:1263363/1‑78 (MQ=255)                                                                         catcatCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAg                                                                                                                      <  1:920149/80‑1 (MQ=255)                                                                         catcatCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAg                                                                                                                      >  2:920149/1‑80 (MQ=255)                                                                          atcatcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTaaaa                                                                                                            >  2:1431841/1‑89 (MQ=255)                                                                          atcatcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTaaaa                                                                                                            <  1:1431841/89‑1 (MQ=255)                                                                              tcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTaaaa                                                                                                            <  2:700673/85‑1 (MQ=255)                                                                              tcGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTaaaa                                                                                                            >  1:700673/1‑85 (MQ=255)                                                                                gggTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGc                                                    <  1:1133772/139‑1 (MQ=255)                                                                                  gTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGc                                                                                                                                    <  1:498128/57‑1 (MQ=255)                                                                                  gTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGccc                                                  >  1:1125542/1‑139 (MQ=255)                                                                                   tGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCttttt                                                                                      <  1:1133508/102‑1 (MQ=255)                                                                                   tGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCttttt                                                                                      >  2:1133508/1‑102 (MQ=255)                                                                                     aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCg                                                                                                       >  1:1412592/1‑83 (MQ=255)                                                                                     aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCg                                                                                                       <  2:1412592/83‑1 (MQ=255)                                                                                     aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCa                                                             <  2:1440705/125‑1 (MQ=255)                                                                                     aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCa                                                             >  1:1440705/1‑125 (MQ=255)                                                                                     aGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCcctc                                               >  2:393693/1‑139 (MQ=255)                                                                                       cGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCcctcct                                             >  1:1203050/1‑139 (MQ=255)                                                                                       cGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCcctcct                                             >  1:519955/1‑139 (MQ=255)                                                                                                 gCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGc                                                    <  2:796214/122‑1 (MQ=255)                                                                                                 gCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGc                                                    >  1:796214/1‑122 (MQ=255)                                                                                                 gCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGAtt                                   >  2:608125/1‑139 (MQ=255)                                                                                                  cACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGc                                                                     >  1:306232/1‑104 (MQ=255)                                                                                                  cACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGc                                                                     <  2:306232/104‑1 (MQ=255)                                                                                                    ccGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAg                                >  1:390759/1‑139 (MQ=255)                                                                                                     cGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCt                                                                                                                    >  2:456635/1‑54 (MQ=255)                                                                                                     cGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCt                                                                                                                    <  1:456635/54‑1 (MQ=255)                                                                                                     cGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                               >  2:1348984/1‑139 (MQ=255)                                                                                                     cGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                               <  2:390759/139‑1 (MQ=255)                                                                                                      gCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGc                                                    <  2:1656632/117‑1 (MQ=255)                                                                                                      gCATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGc                                                    >  1:1656632/1‑117 (MQ=255)                                                                                                       cATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTaaaa                                                                                                            >  2:1771355/1‑60 (MQ=255)                                                                                                       cATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTaaaa                                                                                                            <  1:1771355/60‑1 (MQ=255)                                                                                                       cATTGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCt                             >  2:1496926/1‑139 (MQ=255)                                                                                                         ttGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGt                           <  2:1532245/139‑1 (MQ=255)                                                                                                         ttGTTTGCCGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGt                           <  2:154204/139‑1 (MQ=255)                                                                                                                ccGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAATGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACt                    <  1:1348984/139‑1 (MQ=255)                                                                                                                ccGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACt                    <  2:1125542/139‑1 (MQ=255)                                                                                                                ccGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACt                    <  1:1496926/139‑1 (MQ=255)                                                                                                                ccGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACt                    <  2:519955/139‑1 (MQ=255)                                                                                                                ccGATCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACt                    <  2:1700193/139‑1 (MQ=255)                                                                                                                   aTCTCTTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGt                 >  1:1230543/1‑139 (MQ=255)                                                                                                                      tctTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTc                                                      <  2:231444/99‑1 (MQ=255)                                                                                                                      tctTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTc                                                      >  1:231444/1‑99 (MQ=255)                                                                                                                      tctTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCg                                                     >  2:506057/1‑100 (MQ=255)                                                                                                                      tctTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCg                                                     <  1:506057/100‑1 (MQ=255)                                                                                                                      tctTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                   >  1:1615944/1‑102 (MQ=255)                                                                                                                      tctTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                   <  2:1615944/102‑1 (MQ=255)                                                                                                                      tctTCACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTAtt              >  2:136311/1‑139 (MQ=255)                                                                                                                          cACTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAc          <  1:270993/139‑1 (MQ=255)                                                                                                                           aCTGAGAAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAAcc         <  1:396595/139‑1 (MQ=255)                                                                                                                              gagaAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                   >  1:62761/1‑94 (MQ=255)                                                                                                                              gagaAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGcc                                                   <  2:62761/94‑1 (MQ=255)                                                                                                                              gagaAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGt                           <  2:738548/118‑1 (MQ=255)                                                                                                                              gagaAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGt                           >  1:738548/1‑118 (MQ=255)                                                                                                                              gagaAAGAATCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGa      <  1:136311/139‑1 (MQ=255)                                                                                                                               agaaagcaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                     <  2:957948/123‑1 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGc                                                                     <  2:1604404/75‑1 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGc                                                                     >  1:1604404/1‑75 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                               >  2:1667436/1‑113 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGc                               <  1:1667436/113‑1 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                     >  1:957948/1‑123 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                     <  2:1091606/123‑1 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                     >  1:1091606/1‑123 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAg       >  2:442554/1‑137 (MQ=255)                                                                                                                               agaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAg       <  1:442554/137‑1 (MQ=255)                                                                                                                                gaaagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACt    >  1:261215/1‑139 (MQ=255)                                                                                                                                  aagaaTCGCGACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTaa  <  2:1203050/139‑1 (MQ=255)                                                                                                                                  aagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                     <  1:181741/120‑1 (MQ=255)                                                                                                                                  aagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAAc                     >  2:181741/1‑120 (MQ=255)                                                                                                                                  aagaaTCGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTaa  >  2:1354496/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                     CAGCTGTTGCCGCGGCGTTGAAACTACAAGAAGATGAAAGCTTTACCAACAAGAATATTGTGGTTATTCTACCATCATCGGGTGAGCGTTATTTAAGCACCGCATTGTTTGCCGATCTCTTCACTGAGAAAGAATTGCAACAGTAATGCCAGCTTGTTAAAAATGCGTAAAAAAGCACCTTTTTAGGTGCTTTTTTGTGGCCTGCTTCAAACTTTCGCCCCTCCTGGCATTGATTCAGCCTGTCGGAACTGGTATTTAACCAGACTAA  >  NC_000913/2533235‑2533502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑