Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,574,135	G→A	D57D (GAC→GAT)	eutT ←	cobalamin adenosyltransferase involved in ethanolamine utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,135	0	G	A	100.0%	152.7 / NA	48	D57D (GAC→GAT)	eutT	cobalamin adenosyltransferase involved in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

CCATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT  >  NC_000913/2573999‑2574269                                                                                                                                         |                                                                                                                                       ccATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCatcatc                                                                                                                                      >  1:772189/1‑139 (MQ=255)  cATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTtcatcatca                                                                                                                                     >  2:1334241/1‑139 (MQ=255)  cATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTtcatcatca                                                                                                                                     >  1:132223/1‑139 (MQ=255)                aCAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGcc                                                                                                                       >  1:727544/1‑139 (MQ=255)                   ggTGAGTCAGCGTATACGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTg                                                                                                                    <  2:1793769/139‑1 (MQ=255)                              gTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATaa                                                                                                         >  2:79568/1‑139 (MQ=255)                                aTCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAAc                                                                                                       <  2:1239563/139‑1 (MQ=255)                                   cGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTg                                                                                                    <  2:727544/139‑1 (MQ=255)                                        ttgtggcagccGGGTAGCGACACAGTTTGGAGGACGTCTGCGGATGTTCACCGCTAGTGGTCAGCCAAGGAACGGATTGAGGCTGCTGATGTGCATTATAATCAAACGGGCGGCCCTGCTCGTCAATAAAATTGATgct                                                                                               <  1:1840860/130‑2 (MQ=255)                                         ttttGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  >  2:1058350/1‑119 (MQ=255)                                         ttttGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  <  1:1058350/119‑1 (MQ=255)                                          tttGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                                 >  2:655626/1‑119 (MQ=255)                                          tttGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                                 <  1:655626/119‑1 (MQ=255)                                                     cTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCtt                                                                                  >  2:1735023/1‑139 (MQ=255)                                                                   cGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCg                                                                    >  1:986987/1‑139 (MQ=255)                                                                    gcagcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAAc                                                                                                       <  1:635444/103‑1 (MQ=255)                                                                    gcagcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAAc                                                                                                       >  2:635444/1‑103 (MQ=255)                                                                    gcagcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCgg                                                                   <  1:79568/139‑1 (MQ=255)                                                                      agcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaacaa                                                                                                                                 <  1:1592301/75‑1 (MQ=255)                                                                      agcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaacaa                                                                                                                                 >  2:1592301/1‑75 (MQ=255)                                                                      agcaCGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGc                                                                 >  1:822852/1‑139 (MQ=255)                                                                            ccTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAAGTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAggg                                                           <  1:1334241/139‑1 (MQ=255)                                                                             cTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGc                                                          >  2:784836/1‑139 (MQ=255)                                                                                  gATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                                 >  1:1441530/1‑79 (MQ=255)                                                                                  gATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTc                                                                                                                 <  2:1441530/79‑1 (MQ=255)                                                                                       tCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGAc                                                >  2:1883044/1‑139 (MQ=255)                                                                                       tCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGAc                                                >  2:1793053/1‑139 (MQ=255)                                                                                       tCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGAc                                                <  2:772189/139‑1 (MQ=255)                                                                                              tACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGc                                         <  2:822852/139‑1 (MQ=255)                                                                                                cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaacaa                                                                                                                                 >  1:1021950/1‑49 (MQ=255)                                                                                                cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATcaacaa                                                                                                                                 <  2:1021950/49‑1 (MQ=255)                                                                                                cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGAt                                                                                                  <  2:1298780/80‑1 (MQ=255)                                                                                                cTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGAt                                                                                                  >  1:1298780/1‑80 (MQ=255)                                                                                                       gCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAggg                                     <  1:982990/134‑1 (MQ=255)                                                                                                       gCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAggg                                     >  2:982990/1‑134 (MQ=255)                                                                                                        cccATGAACGGGCTGCGGCTGCTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACGTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGCCGACTGTCCGCAGGGAGATgg                               >  1:1096980/1‑139 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAggcgg                                                                                                                         <  1:263641/39‑1 (MQ=38)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAggcgg                                                                                                                         >  2:263641/1‑39 (MQ=38)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAgg                                                                                                                            <  1:850028/36‑1 (MQ=37)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAgg                                                                                                                            >  2:850028/1‑36 (MQ=37)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc                                                                            <  2:1053409/84‑1 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGc                                                                            >  1:1053409/1‑84 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACt                                               <  2:278975/113‑1 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACt                                               >  1:278975/1‑113 (MQ=255)                                                                                                                  ggctgcggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTgcgc                     >  1:1408168/1‑139 (MQ=255)                                                                                                                  ggcagcggctgcTGCTGTTCTTCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTgcgc                     >  2:1277480/1‑139 (MQ=255)                                                                                                                        ggctgcTGCTGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGc               >  1:1796485/1‑139 (MQ=255)                                                                                                                         gctgctgctGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  <  2:1057763/39‑1 (MQ=255)                                                                                                                         gctgctgctGTTCATCATCAACAAACAGGCGGCCCTGCt                                                                                                                  >  1:1057763/1‑39 (MQ=255)                                                                                                                         gctgctgctGTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCt              <  1:784836/139‑1 (MQ=255)                                                                                                                               gctgTTCATCATCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGt        <  2:1796485/139‑1 (MQ=255)                                                                                                                                    tcatcatcaACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGt   <  2:132223/139‑1 (MQ=255)                                                                                                                                     catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt  <  2:1823179/139‑1 (MQ=255)                                                                                                                                     catcatCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGtt  <  2:986987/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CCATTTTTTCCGCCGACAGGTGAGTCAGCGTATCCGGCTTTTTTGCCACCGGCTGGCGACACAGTTCGCAGCACGCCTGCGGATGTTCATCGCTACTGGTCAGCCCATGAACGGGCTGCGGCTGCTGCTGTTCATCGTCAACAAACAGGCGGCCCTGCTCGTCAATAAACTTGATGCGCAGATGGCGGCTTTCCAGCAACTCCCGGGCAGAGGGCGTCAGGCGACTGTCCGCAGGGAGATGGATCTCTGCGCCTTCGCTGAGCGTATGGTT  >  NC_000913/2573999‑2574269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑