Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,824,491 C→T *362* (TAG→TAA mltB ← membrane‑bound lytic murein transglycosylase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,824,4910CT100.0% 163.0 / NA 50*362* (TAG→TAAmltBmembrane‑bound lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/27);  total (23/27)

TGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAC  >  NC_000913/2824356‑2824599
                                                                                                                                       |                                                                                                            
tGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAc                                                                                                           <  2:842794/139‑1 (MQ=255)
     gtcaAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACttact                                                                                                      <  1:411819/139‑5 (MQ=255)
      tcaAAAATTTATGCCGGACAAGATAGCGCTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTc                                                                                                    >  2:1428607/1‑139 (MQ=255)
                aTGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCgcgcc                                                                                               <  1:644258/134‑1 (MQ=255)
                aTGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCgcgcc                                                                                               >  2:644258/1‑134 (MQ=255)
                     ggACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                     <  1:1579785/139‑1 (MQ=255)
                       aCAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCt                                                                                   <  1:124017/139‑1 (MQ=255)
                                     tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGATGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTc                                                                               <  2:1189774/129‑1 (MQ=255)
                                     tCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCCCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTc                                                                               >  1:1189774/1‑129 (MQ=255)
                                                 tGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGc                                                         >  1:1287968/1‑139 (MQ=255)
                                                         gCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                 >  2:1866469/1‑139 (MQ=255)
                                                           gTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                    >  2:365026/1‑102 (MQ=255)
                                                           gTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                    <  1:365026/102‑1 (MQ=255)
                                                            ttGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                              <  2:1287968/139‑1 (MQ=255)
                                                             tGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCt                                             <  2:1858004/139‑1 (MQ=255)
                                                              gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                     >  1:99141/1‑98 (MQ=255)
                                                              gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                     <  2:99141/98‑1 (MQ=255)
                                                              gCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCtg                                            >  1:350769/1‑139 (MQ=255)
                                                                            aggaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                 <  2:465586/120‑1 (MQ=255)
                                                                            aggaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                 >  1:465586/1‑120 (MQ=255)
                                                                              gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCg                                                                >  2:1816234/1‑103 (MQ=255)
                                                                              gaTTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCg                                                                <  1:1816234/103‑1 (MQ=255)
                                                                                        aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAc                                                                                                           <  1:1481163/51‑1 (MQ=255)
                                                                                        aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTAc                                                                                                           >  2:1481163/1‑51 (MQ=255)
                                                                                        aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                  >  1:1827063/1‑91 (MQ=255)
                                                                                        aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                  >  1:143825/1‑91 (MQ=255)
                                                                                        aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                  <  2:143825/91‑1 (MQ=255)
                                                                                        aaaGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                  <  2:1827063/91‑1 (MQ=255)
                                                                                                      agagGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGt    <  1:1866469/139‑1 (MQ=255)
                                                                                                       gagGGAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTa   <  2:1088083/139‑1 (MQ=255)
                                                                                                         gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                  <  1:965703/74‑1 (MQ=255)
                                                                                                         gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGAc                                                                  >  2:965703/1‑74 (MQ=255)
                                                                                                         gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGt                              >  1:167722/1‑110 (MQ=255)
                                                                                                         gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGt                              <  2:167722/110‑1 (MQ=255)
                                                                                                         gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            <  1:1359687/112‑1 (MQ=255)
                                                                                                         gggAAGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGa                            >  2:1359687/1‑112 (MQ=255)
                                                                                                             aGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                              >  1:959125/1‑90 (MQ=255)
                                                                                                             aGCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                              <  2:959125/90‑1 (MQ=255)
                                                                                                              gCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                    >  2:1785203/1‑51 (MQ=38)
                                                                                                              gCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGc                                                                                    <  1:1785203/51‑1 (MQ=38)
                                                                                                              gCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                              >  2:579073/1‑89 (MQ=255)
                                                                                                              gCTCACGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGc                                              <  1:579073/89‑1 (MQ=255)
                                                                                                                  aCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                     <  2:1596420/46‑1 (MQ=38)
                                                                                                                  aCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACgg                                                                                     >  1:1596420/1‑46 (MQ=38)
                                                                                                                  aCGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATggg                                                 <  2:1087178/82‑1 (MQ=255)
                                                                                                                  aCGCCCCCCTCTTGTAAATAGTTACTTACACGCGCCAGCGCCACGGCTTGTCATAACTGCCAGACCGCCATTGCGTAATggg                                                 >  1:1087178/1‑82 (MQ=255)
                                                                                                                   cGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGt                                        <  2:921213/90‑1 (MQ=255)
                                                                                                                   cGCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGt                                        >  1:921213/1‑90 (MQ=255)
                                                                                                                    gCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc  >  2:825914/1‑127 (MQ=255)
                                                                                                                    gCCCCCCTCTTGTAAATAGTTACTTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAc  <  1:825914/127‑1 (MQ=255)
                                                                                                                                       |                                                                                                            
TGTCAGTCAAAAATTTATGCCGGACAAGATAGCACTTTCGTCAGGAAGATGGTGTTGGCGTTGCAATTTTACGAGGAGGATTCAGAAAAAAGCTGATTAGCCAGAGGGAAGCTCACGCCCCCCTCTTGTAAATAGCTACTGTACTCGCGCCAGCGCCACGGCTTGTCCTAACTGCCAGACCGCCATTGCGTAATGGGTGCTGTGGTTGTAACGGGTGATGGTGTAGAAGTTCGGCAGACCGTAC  >  NC_000913/2824356‑2824599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: