Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,851,873 G→A *291* (TAG→TAA) 
G4S (GGC→AGC) 		 hypB →
hypC →		 GTP hydrolase involved in nickel liganding into hydrogenases
hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,851,8730GA100.0% 200.5 / NA 62*291* (TAG→TAA) 
G4S (GGC→AGC) 		hypB
hypC		GTP hydrolase involved in nickel liganding into hydrogenases
hydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/31);  total (31/31)

GACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGT  >  NC_000913/2851745‑2852006
                                                                                                                                |                                                                                                                                     
gACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCgg                                                                                                                             >  2:675707/1‑139 (MQ=255)
 aCGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGc                                                                                                                            <  1:675707/139‑1 (MQ=255)
 aCGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCCTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTc                                                                                                                                 <  1:411176/134‑1 (MQ=255)
 aCGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCCTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTc                                                                                                                                 >  2:411176/1‑134 (MQ=255)
         aaGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCg                                                                                                                    <  1:1313298/139‑1 (MQ=255)
                   ccTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGAc                                                                                                          >  2:582393/1‑139 (MQ=255)
                         cccGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAc                                                                                                    <  2:1132249/139‑1 (MQ=255)
                              gAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGc                                                                                               <  2:1474030/139‑1 (MQ=255)
                                 gTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGaa                                                                                            >  2:51389/1‑139 (MQ=255)
                                          gAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt                                                                                   <  1:1100444/139‑1 (MQ=255)
                                          gAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGt                                                                                   <  2:1036981/139‑1 (MQ=255)
                                           aaaTTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATATGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAc                                                                                                    <  1:1553149/121‑1 (MQ=255)
                                           aaaTTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATATGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAc                                                                                                    >  2:1553149/1‑121 (MQ=255)
                                            aaTTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCt                                                                                 >  2:1745376/1‑139 (MQ=255)
                                            aaTTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCt                                                                                 <  1:909189/139‑1 (MQ=255)
                                                gAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCaa                                                                                                     >  2:45785/1‑115 (MQ=255)
                                                gAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCaa                                                                                                     <  1:45785/115‑1 (MQ=255)
                                                gAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCgg                                                                             >  2:1223396/1‑139 (MQ=255)
                                                gAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCg                                                                              <  2:1687093/138‑1 (MQ=255)
                                                gAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCg                                                                              >  1:1687093/1‑138 (MQ=255)
                                                     catcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTc                                                                        <  1:1745376/139‑1 (MQ=255)
                                                       tcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCa                                                                                                                  >  1:1795560/1‑95 (MQ=255)
                                                       tcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCa                                                                                                                  <  2:1795560/95‑1 (MQ=255)
                                                       tcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGa                                                                                             <  2:1525746/116‑1 (MQ=255)
                                                       tcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGa                                                                                             >  1:1525746/1‑116 (MQ=255)
                                                       tcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCa                                                                           >  1:1158135/1‑134 (MQ=255)
                                                       tcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCa                                                                           <  2:1158135/134‑1 (MQ=255)
                                                       tcCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAg                                                                      >  1:1712078/1‑139 (MQ=255)
                                                        ccTTATTTCCGCCACCAGCGGAGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATACGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAgc                                                                     >  1:1130136/1‑139 (MQ=255)
                                                           tATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGcgcg                                                                  >  2:1732101/1‑139 (MQ=255)
                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACg                                                                                                         <  1:435251/93‑1 (MQ=255)
                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACg                                                                                                         >  2:435251/1‑93 (MQ=255)
                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAc                                                                                                    >  2:573974/1‑98 (MQ=255)
                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAAc                                                                                                    <  1:573974/98‑1 (MQ=255)
                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGa                                                           <  2:1130136/139‑1 (MQ=255)
                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGa                                                           >  1:1166138/1‑139 (MQ=255)
                                                                  gCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGa                                                           >  2:1707166/1‑139 (MQ=255)
                                                                   ccaccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCa                                                                                                               >  1:589572/1‑86 (MQ=255)
                                                                   ccaccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCa                                                                                                               <  2:589572/86‑1 (MQ=255)
                                                                    caccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGAtt                                                         <  2:364812/139‑1 (MQ=255)
                                                                    caccaGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGACTGCGGCATTCAGCGCGATGTCGAtt                                                         >  1:136218/1‑139 (MQ=255)
                                                                       caGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTaa                                                      <  1:1223396/139‑1 (MQ=255)
                                                                          cggcgAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGAc                                                                                     >  2:1114640/1‑105 (MQ=255)
                                                                          cggcgAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGAc                                                                                     <  1:1114640/105‑1 (MQ=255)
                                                                          cggcgAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATg                                                               >  2:941267/1‑127 (MQ=255)
                                                                          cggcgAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATg                                                               <  1:941267/127‑1 (MQ=255)
                                                                          cggcgAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGt                                                   >  1:614291/1‑139 (MQ=255)
                                                                                aGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCg                                             >  1:893899/1‑139 (MQ=255)
                                                                                             tGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCaa                                                                                                     >  1:312751/1‑70 (MQ=255)
                                                                                             tGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCaa                                                                                                     <  2:312751/70‑1 (MQ=255)
                                                                                             tGGCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGa                                >  1:1869823/1‑139 (MQ=255)
                                                                                               gCTGAACTGGCTGGAGACACAGCGATTTGCATAAGCGTTCCCGGCCAGATCCGCa                                                                                                                  <  2:1164183/55‑1 (MQ=255)
                                                                                               gCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCa                                                                                                                  >  1:1164183/1‑55 (MQ=255)
                                                                                               gCTGAACTGGCTGGAGACACAGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGaaa                              <  1:582393/139‑1 (MQ=255)
                                                                                                                  caGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGt                                                              <  2:1781473/88‑1 (MQ=255)
                                                                                                                  caGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGt                                                              >  1:1781473/1‑88 (MQ=255)
                                                                                                                   aGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGAtt                                                         >  2:1457084/1‑92 (MQ=255)
                                                                                                                   aGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGAtt                                                         <  1:1457084/92‑1 (MQ=255)
                                                                                                                   aGCGATGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGc          <  1:486551/139‑1 (MQ=255)
                                                                                                                       aTGTGCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGACTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGt      <  2:136218/139‑1 (MQ=255)
                                                                                                                         gtgCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACTGTCAGCCGCGCGTGGGCCAGTgg    >  2:1642318/1‑139 (MQ=255)
                                                                                                                           gCATAAGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGt  <  1:51389/139‑1 (MQ=255)
                                                                                                                                |                                                                                                                                     
GACGTTGAAAAGTGCATCGCCTGCGCCCGCGAAGTCAATCCAGAAATTGAAATCATCCTTATTTCCGCCACCAGCGGCGAAGGGATGGACCAGTGGCTGAACTGGCTGGAGACACAGCGATGTGCATAGGCGTTCCCGGCCAGATCCGCACCATTGACGGCAACCAGGCGAAAGTCGACGTCTGCGGCATTCAGCGCGATGTCGATTTAACGTTAGTCGGCAGCTGCGATGAAAACGGTCAGCCGCGCGTGGGCCAGTGGGT  >  NC_000913/2851745‑2852006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑