Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,915,105 C→T P17S (CCG→TCG)  barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,915,1050CT98.7% 254.2 / NA 78P17S (CCG→TCG) barAhybrid sensory histidine kinase, in two‑component regulatory system with UvrY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (34/43);  minor base A (1/0);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 4.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCACCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATG  >  NC_000913/2914969‑2915236
                                                                                                                                        |                                                                                                                                   
gtGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcg                                                                                                                                   <  1:1140930/139‑1 (MQ=255)
   cgtcgtTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                <  2:916670/139‑1 (MQ=255)
   cgtcgtTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                <  1:1723973/139‑1 (MQ=255)
     tcgtTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGt                                                                                                                              >  1:1432372/1‑139 (MQ=255)
        ttttGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                >  2:1098175/1‑134 (MQ=255)
        ttttGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                <  1:1098175/134‑1 (MQ=255)
             cAGAATAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGCACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTg                                                                                                                      >  1:835308/1‑139 (MQ=255)
               gagTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGt                                                                                                                    <  2:1432372/139‑1 (MQ=255)
                agTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGtt                                                                                                                   <  1:1404654/139‑1 (MQ=255)
                         gcgcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGa                                                                                                          <  2:266533/139‑1 (MQ=255)
                           gcCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGt                                                                                                        <  1:1354973/139‑1 (MQ=255)
                                     agaCTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTAt                                                                                                      >  2:1523746/1‑131 (MQ=255)
                                     agaCTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTAt                                                                                                      <  1:1523746/131‑1 (MQ=255)
                                     agaCTTTCTCAATTTAACAGTGTGACCTTAATTGCCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTtcg                                                                                              <  2:276584/139‑1 (MQ=255)
                                         tttCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAt                                                                                                                <  2:1031879/117‑1 (MQ=255)
                                         tttCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAt                                                                                                                >  1:1031879/1‑117 (MQ=255)
                                          ttCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                         <  1:127895/139‑1 (MQ=255)
                                           tctcAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGc                                                                                        <  1:1699319/139‑1 (MQ=255)
                                             tcAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                      <  2:1145234/139‑1 (MQ=255)
                                                 tttAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCt                                                                                  >  1:1654969/1‑139 (MQ=255)
                                                         tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGt                                                                                                                              >  2:1809961/1‑87 (MQ=255)
                                                         tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGt                                                                                                                              <  1:1809961/87‑1 (MQ=255)
                                                         tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTa                                                                                                                 <  1:808889/100‑1 (MQ=255)
                                                         tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTa                                                                                                                 >  2:808889/1‑100 (MQ=255)
                                                         tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCtttt                                                                                                 >  2:623390/1‑116 (MQ=255)
                                                         tgtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCtttt                                                                                                 <  1:623390/116‑1 (MQ=255)
                                                          gtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                               <  1:154722/85‑1 (MQ=255)
                                                          gtgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                               >  2:154722/1‑85 (MQ=255)
                                                           tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                               <  2:1691883/84‑1 (MQ=255)
                                                           tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                               >  1:1691883/1‑84 (MQ=255)
                                                           tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                >  2:517059/1‑83 (MQ=255)
                                                           tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                <  1:517059/83‑1 (MQ=255)
                                                           tgACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACtt                                                                        >  1:923970/1‑139 (MQ=255)
                                                                       tCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACt                                                            >  2:582230/1‑139 (MQ=255)
                                                                        cccATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGt                                                                                                        >  1:888944/1‑94 (MQ=255)
                                                                        cccATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGt                                                                                                        <  2:888944/94‑1 (MQ=255)
                                                                            tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGt                                                                                                                    >  2:896836/1‑78 (MQ=255)
                                                                            tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGt                                                                                                                    <  1:896836/78‑1 (MQ=255)
                                                                            tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGc                                                                                                             <  1:1642070/85‑1 (MQ=255)
                                                                            tAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGc                                                                                                             >  2:1642070/1‑85 (MQ=255)
                                                                                 gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                <  2:805690/61‑1 (MQ=255)
                                                                                 gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                >  1:805690/1‑61 (MQ=255)
                                                                                 gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                  >  2:1013567/1‑59 (MQ=255)
                                                                                 gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                  <  1:1013567/59‑1 (MQ=255)
                                                                                 gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCt                                                                                                            >  2:273831/1‑81 (MQ=255)
                                                                                 gAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCt                                                                                                            <  1:273831/81‑1 (MQ=255)
                                                                                  aaCTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                <  2:1287207/60‑1 (MQ=255)
                                                                                  aaCTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgacc                                                                                                                                >  1:1287207/1‑60 (MQ=255)
                                                                                  aaCTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGc                                                   <  2:109160/137‑1 (MQ=255)
                                                                                  aaCTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGc                                                   >  1:109160/1‑137 (MQ=255)
                                                                                      ccATGACCAACTACAGCCTGCGCGCACGTATGATGTTTCTGATCTTGTCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGc                                             <  1:687588/139‑1 (MQ=255)
                                                                                       cATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGtt                                                                                                                   >  2:29688/1‑68 (MQ=255)
                                                                                       cATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGtt                                                                                                                   <  1:29688/68‑1 (MQ=255)
                                                                                       cATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGt                                                                                                        >  1:774224/1‑79 (MQ=255)
                                                                                       cATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGt                                                                                                        <  2:774224/79‑1 (MQ=255)
                                                                                       cATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                      <  1:1644324/97‑1 (MQ=255)
                                                                                       cATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                      >  2:1644324/1‑97 (MQ=255)
                                                                                         tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                               <  1:1603286/54‑1 (MQ=255)
                                                                                         tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcgaccg                                                                                                                               >  2:1603286/1‑54 (MQ=255)
                                                                                         tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAt                                                                                                                <  1:1456324/69‑1 (MQ=255)
                                                                                         tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTAt                                                                                                                >  2:1456324/1‑69 (MQ=255)
                                                                                         tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                         <  1:620316/92‑1 (MQ=255)
                                                                                         tGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                         >  2:620316/1‑92 (MQ=255)
                                                                                         agacagaCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCTTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGGGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAg                                          <  1:624043/133‑1 (MQ=255)
                                                                                               aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                  <  1:330171/45‑1 (MQ=255)
                                                                                               aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCatcga                                                                                                                                  >  2:330171/1‑45 (MQ=255)
                                                                                               aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTc                                                                                                                             >  1:825139/1‑50 (MQ=255)
                                                                                               aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTc                                                                                                                             <  2:825139/50‑1 (MQ=255)
                                                                                               aCTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCattat                                    >  1:101950/1‑139 (MQ=255)
                                                                                                      ccTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCg                             >  1:1034675/1‑139 (MQ=255)
                                                                                                       cTGCGCGCACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGc                            <  2:835308/139‑1 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGCTCCAGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGcctgtct                         >  2:624043/1‑134 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTc                                                                                                                             <  2:1557570/39‑1 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTc                                                                                                                             >  1:1557570/1‑39 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                      <  2:909039/78‑1 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCAt                                                                                      >  1:909039/1‑78 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATc                                                                                     <  2:944512/79‑1 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATc                                                                                     >  1:944512/1‑79 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACtt                                                                        <  1:651543/92‑1 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACtt                                                                        >  2:651543/1‑92 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                          >  1:51937/1‑138 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCtt                          <  2:51937/138‑1 (MQ=255)
                                                                                                          cgcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTg                         >  1:1011344/1‑139 (MQ=255)
                                                                                                           gcgcACGCATGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGc                        >  2:1756298/1‑139 (MQ=255)
                                                                                                              cacgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGt                     <  2:1034675/139‑1 (MQ=255)
                                                                                                              cacgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGt                     <  1:582230/139‑1 (MQ=255)
                                                                                                              cacgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGt                     <  2:101950/139‑1 (MQ=255)
                                                                                                              cacgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGt                     <  1:1756298/139‑1 (MQ=255)
                                                                                                              agagacagatgatTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGt                     <  1:1145874/132‑1 (MQ=255)
                                                                                                                cgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGttt                   <  2:1190370/139‑1 (MQ=255)
                                                                                                                cgcaTGATGATTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGttt                   <  2:923970/139‑1 (MQ=255)
                                                                                                                         atTCTGATCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAat          <  2:1654969/139‑1 (MQ=255)
                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                         >  1:1791708/1‑54 (MQ=255)
                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTg                                                                                         <  2:1791708/54‑1 (MQ=255)
                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAg                                          >  2:1323452/1‑101 (MQ=255)
                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAg                                          <  1:1323452/101‑1 (MQ=255)
                                                                                                                               aTCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCa    >  2:407127/1‑139 (MQ=255)
                                                                                                                                tCCTGGCATCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCAt   <  1:407127/139‑1 (MQ=255)
                                                                                                                                 ccTGGCAACGACCGTCCTTATTGGATTATTGCTGAGTATCATTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAatctgtctct  >  2:1145874/1‑131 (MQ=255)
                                                                                                                                        |                                                                                                                                   
GTGCGTCGTTTTGCAGAGTAGAATTGCGCCATTGGCGAGACTTTCTCAATTTAACAGTGTGACCTTAATTGTCCCATAACGGAACTCCATGACCAACTACAGCCTGCGCGCACGCATGATGATTCTGATCCTGGCACCGACCGTCCTTATTGGTTTATTGCTGAGTATCTTTTTCGTCGTGCATCGCTATAACGACTTGCAGCGTCAACTGGAAGATGCCGGTGCCAGCATTATTGAGCCGCTTGCAGTTTCTACTGAATATGGCATG  >  NC_000913/2914969‑2915236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: