Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,050,691	G→A	intergenic (‑24/+424)	gcvT ← / ← ubiI	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,050,691	0	G	A	100.0%	196.7 / NA	60	intergenic (‑24/+424)	gcvT/ubiI	aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/34);  total (26/34)

GATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATC  >  NC_000913/3050556‑3050825                                                                                                                                        |                                                                                                                                       gatgTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGTGAGCGCCGCCAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTTTGCCATCTTGTCCTCATTGAATAAGCGGGACTg                                                                                                                                     >  1:784184/1‑139 (MQ=255) gatgTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTg                                                                                                                                     <  2:1331129/139‑1 (MQ=255)   tgTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGAc                                                                                                                                   <  2:63668/139‑1 (MQ=255)                                cGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACg                                                                                                      <  2:1468879/139‑1 (MQ=255)                                             ccGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCg                                                                                         <  2:1329597/139‑1 (MQ=255)                                                tGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAg                                                                                      <  2:1608382/139‑1 (MQ=255)                                                tGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAg                                                                                      >  1:1608382/1‑139 (MQ=255)                                                 ggAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGc                                                                                     <  1:757337/139‑1 (MQ=255)                                                                 aGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGaaa                                                                                                   <  2:1492566/109‑1 (MQ=255)                                                                 aGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGaaa                                                                                                   >  1:1492566/1‑109 (MQ=255)                                                                 aGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCaa                                                                     >  1:1138594/1‑139 (MQ=255)                                                                      cgcAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCaa                                                                <  1:74685/139‑1 (MQ=255)                                                                        cAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACt                                                                                                                                      >  1:1474431/1‑67 (MQ=255)                                                                        cAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACt                                                                                                                                      <  2:1474431/67‑1 (MQ=255)                                                                                gtgtTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCtt                                                      <  2:1138594/139‑1 (MQ=255)                                                                                    tgttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGc                                                  <  1:247397/139‑1 (MQ=255)                                                                                    tgttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGc                                                  <  1:1276778/139‑1 (MQ=255)                                                                                      ttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCt                                                <  1:1581983/139‑1 (MQ=255)                                                                                      ttCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCt                                                >  2:653267/1‑139 (MQ=255)                                                                                       tCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCAc                                                                                  <  2:652008/104‑1 (MQ=255)                                                                                       tCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCAc                                                                                  >  1:652008/1‑104 (MQ=255)                                                                                        cGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGa                                              >  2:1758876/1‑139 (MQ=255)                                                                                          tACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGc                                                                  <  1:780378/117‑1 (MQ=255)                                                                                          tACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGc                                                                  >  2:780378/1‑117 (MQ=255)                                                                                           aCAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACt                                           >  1:1130474/1‑139 (MQ=255)                                                                                           aCAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACt                                           >  2:867280/1‑139 (MQ=255)                                                                                               aGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTacc                                       >  2:110017/1‑139 (MQ=255)                                                                                                        ttgtGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACa                              <  1:867280/139‑1 (MQ=255)                                                                                                        ttgtGCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACa                              <  2:1130474/139‑1 (MQ=255)                                                                                                          gtgCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGa                            <  1:110017/139‑1 (MQ=255)                                                                                                          gtgCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGa                            <  2:1790129/139‑1 (MQ=255)                                                                                                           tgCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGAc                           >  2:1805534/1‑139 (MQ=255)                                                                                                           tgCCATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCA‑CACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACt                          >  2:841093/1‑139 (MQ=255)                                                                                                             ccATCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTg                         >  2:295242/1‑139 (MQ=255)                                                                                                                tCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCa                                                                                                                         <  1:33388/40‑1 (MQ=255)                                                                                                                tCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCa                                                                                                                         >  2:33388/1‑40 (MQ=255)                                                                                                                tCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTacca                                      <  2:806904/123‑1 (MQ=255)                                                                                                                tCTTGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGAAATCGTTCTCTTTTGCCTGAACTTacca                                      >  1:806904/1‑123 (MQ=255)                                                                                                                   tGTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCA‑CACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCa                  <  1:841093/139‑1 (MQ=255)                                                                                                                    gTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAGCTTACCACCGAAACAGACTGTTAACCa                  <  2:784184/139‑1 (MQ=255)                                                                                                                    gTCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCa                  <  1:975562/139‑1 (MQ=255)                                                                                                                     tCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACg                                                                                                      >  1:732025/1‑54 (MQ=255)                                                                                                                     tCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACg                                                                                                      <  2:732025/54‑1 (MQ=255)                                                                                                                     tCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACg                                                                   <  1:1138299/89‑1 (MQ=255)                                                                                                                     tCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACg                                                                   >  2:1138299/1‑89 (MQ=255)                                                                                                                     tCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTaccac                                     >  2:809270/1‑119 (MQ=255)                                                                                                                     tCCTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTaccac                                     <  1:809270/119‑1 (MQ=255)                                                                                                                       cTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTacca                                      <  2:1651917/116‑1 (MQ=255)                                                                                                                       cTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTacca                                      >  1:1651917/1‑116 (MQ=255)                                                                                                                       cTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGa                            >  1:621025/1‑126 (MQ=255)                                                                                                                       cTCATTGAATAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGa                            <  2:621025/126‑1 (MQ=255)                                                                                                                               aTAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCg                                                                             <  2:597765/69‑1 (MQ=255)                                                                                                                               aTAAGCGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCg                                                                             >  1:597765/1‑69 (MQ=255)                                                                                                                                    cGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACg                                                                   >  1:1402256/1‑74 (MQ=255)                                                                                                                                    cGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACg                                                                   <  2:1402256/74‑1 (MQ=255)                                                                                                                                    cGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGc                                                  <  1:1539120/91‑1 (MQ=255)                                                                                                                                    cGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGc                                                  >  2:1539120/1‑91 (MQ=255)                                                                                                                                    cGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatc  >  2:496538/1‑139 (MQ=255)                                                                                                                                    cGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatc  <  1:653267/139‑1 (MQ=255)                                                                                                                                    cGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatc  >  1:352878/1‑139 (MQ=255)                                                                                                                                    cGGGACTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGatc  <  1:496538/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       GATGTTCGTCGATTTGCGAACCGTAATGCAGCGGCATCATCCAGCCGTGGAAATCCACCATGCGAGCGCCGCAAAGCGTGTGTTGTTCGTACAAAGGAGTCTGTTGTGCCATCTTGTCCTCATTGAATAAGCGGGGCTGACAACTTTTTCATGGTGAAATTATCACCACGAAACCCAGCATCGGAGCCACTCCCGGTCCCCAACGCAATCGTTCTCTTTTGCCTGAACTTACCACCGAAACAGACTGTTAACCATAAGGTAAAATTGATC  >  NC_000913/3050556‑3050825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑