Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,096,673 G→A *97* (TAG→TAA yggU → UPF0235 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,096,6730GA100.0% 105.9 / NA 33*97* (TAG→TAAyggUUPF0235 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/16);  total (17/16)

GTTTCTCGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAA  >  NC_000913/3096541‑3096804
                                                                                                                                    |                                                                                                                                   
gTTTCTCGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATcc                                                                                                                               <  2:1408309/139‑1 (MQ=255)
      cGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCCTTAATTAATTAAGTATCCTATGCa                                                                                                                         <  1:1275860/139‑1 (MQ=255)
          aaGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCaaaaa                                                                                                                     >  2:1681102/1‑139 (MQ=255)
                  ccGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCt                                                                                                             <  1:803344/139‑1 (MQ=255)
                     ggTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCt                                                                                                             <  2:616098/136‑1 (MQ=255)
                     ggTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCt                                                                                                             >  1:616098/1‑136 (MQ=255)
                      gTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCa                                                                                                         <  1:133743/139‑1 (MQ=255)
                            aaaaGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCACAACCGGc                                                                                                   <  2:1079026/139‑1 (MQ=255)
                                                         aCTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCCGAAATCGCGGCGTTAATTAATGAAATATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTc                                                                      >  2:1150662/1‑139 (MQ=255)
                                                                                    cATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGatat                                           >  1:496798/1‑139 (MQ=255)
                                                                                      ttAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCg                                         >  1:1681875/1‑139 (MQ=255)
                                                                                          tCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACt                                                        >  1:660865/1‑120 (MQ=255)
                                                                                          tCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACt                                                        <  2:660865/120‑1 (MQ=255)
                                                                                               aacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATcc                                                                                                                               >  1:598824/1‑44 (MQ=255)
                                                                                               aacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATcc                                                                                                                               <  2:598824/44‑1 (MQ=255)
                                                                                               aacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCt                                                                                                                              >  2:1378109/1‑45 (MQ=255)
                                                                                               aacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCt                                                                                                                              <  1:1378109/45‑1 (MQ=255)
                                                                                               aacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCaaa                                >  2:1726990/1‑139 (MQ=255)
                                                                                                 caaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTc                                                                                                            >  2:991782/1‑61 (MQ=255)
                                                                                                 caaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTc                                                                                                            <  1:991782/61‑1 (MQ=255)
                                                                                                    aTCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCt                                                 <  2:1005516/117‑1 (MQ=255)
                                                                                                    aTCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCt                                                 >  1:1005516/1‑117 (MQ=255)
                                                                                                         gCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGc                                                                                   <  2:1203603/78‑1 (MQ=255)
                                                                                                         gCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGc                                                                                   >  1:819824/1‑78 (MQ=255)
                                                                                                         gCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGc                                                                                   >  1:1203603/1‑78 (MQ=255)
                                                                                                         gCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGc                                                                                   <  2:819824/78‑1 (MQ=255)
                                                                                                           cAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCg                    >  2:57148/1‑139 (MQ=255)
                                                                                                              aaaTCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATc                                          <  2:1695268/114‑1 (MQ=255)
                                                                                                              aaaTCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATc                                          >  1:1695268/1‑114 (MQ=255)
                                                                                                                        gTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCgg                                                                                           <  2:1398159/55‑1 (MQ=255)
                                                                                                                        gTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCgg                                                                                           >  1:1398159/1‑55 (MQ=255)
                                                                                                                            attaattaAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGaaa  <  1:1681102/139‑1 (MQ=255)
                                                                                                                             ttaattaaGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGaaa  <  2:1681875/139‑1 (MQ=255)
                                                                                                                                 ttaAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGt                                                   >  2:1652574/1‑86 (MQ=255)
                                                                                                                                    |                                                                                                                                   
GTTTCTCGGTAAGCAATTCCGGGTTGCCAAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAA  >  NC_000913/3096541‑3096804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: