Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,131,341	C→T	*356* (TAG→TAA)	yghQ ←	putative inner membrane polysaccharide flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,131,341	0	C	T	100.0%	155.0 / NA	48	*356* (TAG→TAA)	yghQ	putative inner membrane polysaccharide flippase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAAC  >  NC_000913/3131211‑3131476                                                                                                                                   |                                                                                                                                        cTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAg                                                                                                                                 >  2:417958/1‑139 (MQ=14)     aaTGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCa                                                                                                                             <  2:1723639/139‑1 (MQ=21)                aaCGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                                  <  2:127530/139‑1 (MQ=255)                        aaaTAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTaa                                                                                                          <  1:1773984/139‑1 (MQ=255)                             ggCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTATCGCCa                                                                                                     <  2:911989/139‑1 (MQ=255)                              gCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                                       <  2:262607/120‑1 (MQ=255)                              gCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                                       >  1:262607/1‑120 (MQ=255)                              gCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGcc                                                                                                      >  1:1054230/1‑137 (MQ=255)                              gCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGcc                                                                                                      <  2:1054230/137‑1 (MQ=255)                               cTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATa                                                                                                                           <  2:454371/115‑1 (MQ=37)                               cTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATa                                                                                                                           >  1:454371/1‑115 (MQ=37)                                 gATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAaca                                                                                                 >  2:1848301/1‑139 (MQ=255)                                 gATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAaca                                                                                                 <  1:417958/139‑1 (MQ=255)                                            tttACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                                  >  2:513006/1‑111 (MQ=255)                                            tttACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                                  <  1:513006/111‑1 (MQ=255)                                                      aaaaaaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGc                                                                            >  1:554721/1‑139 (MQ=255)                                                                     aCATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACcg                                                             >  1:741036/1‑139 (MQ=255)                                                                         gaagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                                       <  1:1205655/77‑1 (MQ=255)                                                                         gaagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCt                                                                                                                       >  2:1205655/1‑77 (MQ=255)                                                                          aagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGaa                                                                                         >  2:271598/1‑106 (MQ=255)                                                                          aagaaATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGaa                                                                                         <  1:271598/106‑1 (MQ=255)                                                                                    atgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAgc                                                        >  2:305308/1‑129 (MQ=255)                                                                                    atgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAgc                                                        <  1:305308/129‑1 (MQ=255)                                                                                       aCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATAc                                           >  1:40444/1‑139 (MQ=255)                                                                                       aCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATAc                                           >  2:1635847/1‑139 (MQ=255)                                                                                       aCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATAc                                           >  1:229711/1‑139 (MQ=255)                                                                                         tGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTa                                                                                                           >  2:1238309/1‑73 (MQ=255)                                                                                         tGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTa                                                                                                           <  1:1238309/73‑1 (MQ=255)                                                                                                 ccGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                                  >  2:434255/1‑58 (MQ=255)                                                                                                 ccGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                                  <  1:434255/58‑1 (MQ=255)                                                                                                  cGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                                  >  1:194143/1‑57 (MQ=255)                                                                                                  cGAGAAGAATTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCgag                                                                                                                  <  2:194143/57‑1 (MQ=255)                                                                                                      aagaaTTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAgcgc                                                      <  2:831017/113‑1 (MQ=255)                                                                                                      aagaaTTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAgcgc                                                      >  1:831017/1‑113 (MQ=255)                                                                                                      aagaaTTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCa                            <  2:40444/139‑1 (MQ=255)                                                                                                      aagaaTTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCa                            <  2:229711/139‑1 (MQ=255)                                                                                                       agaaTTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAg                                                         >  1:1519013/1‑109 (MQ=255)                                                                                                       agaaTTTCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAg                                                         <  2:1519013/109‑1 (MQ=255)                                                                                                           tttCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCt                                                                               >  1:109468/1‑83 (MQ=255)                                                                                                           tttCCCCGCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCt                                                                               <  2:109468/83‑1 (MQ=255)                                                                                                                  gCTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACt                <  2:741036/139‑1 (MQ=255)                                                                                                                   cTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACtt               >  2:1444413/1‑139 (MQ=255)                                                                                                                   cTTATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACtt               >  1:1313303/1‑139 (MQ=255)                                                                                                                     tATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTca             <  2:157773/139‑1 (MQ=255)                                                                                                                     tATTCGCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTca             <  1:1444413/139‑1 (MQ=255)                                                                                                                          gCACCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCt        <  1:1635847/139‑1 (MQ=255)                                                                                                                            aCCTTCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACTGGACTTCACACCTaa      <  2:554721/139‑1 (MQ=255)                                                                                                                                tCCTTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTaacaac  >  2:111360/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                        CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAAC  >  NC_000913/3131211‑3131476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑