Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,140,294	T→C	S9G (AGC→GGC)	hybF ←	protein involved with the maturation of hydrogenases 1 and 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,140,294	0	T	C	100.0%	121.6 / NA	35	S9G (AGC→GGC)	hybF	protein involved with the maturation of hydrogenases 1 and 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/15);  total (20/15)

CCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGG  >  NC_000913/3140158‑3140425                                                                                                                                         |                                                                                                                                    ccGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGccct                                                                                                                                   <  1:1407218/139‑1 (MQ=255)      gCAGACAATTTCAAAACTATAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCaa                                                                                                                              >  2:1255342/1‑139 (MQ=255)                    aaCTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATg                                                                                                                >  2:967238/1‑139 (MQ=255)                           acggacggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTc                                                                                                         >  2:1620859/1‑139 (MQ=255)                             ggacggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCTAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGc                                                                                                       <  1:1769518/139‑1 (MQ=255)                               acggCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCa                                                                                                     <  1:1620859/139‑1 (MQ=255)                                     ctctCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGTGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTg                                                                                               <  1:1255342/139‑1 (MQ=255)                                     ctctCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTg                                                                                               >  2:1433982/1‑139 (MQ=255)                                             aaCGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGAGACCaaa                                                                                       >  2:1757983/1‑139 (MQ=255)                                               cGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAca                                                                                     >  2:1875570/1‑139 (MQ=255)                                                  aGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAAcagca                                                                                  <  1:32721/139‑1 (MQ=255)                                                    gagaGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCa                                                                                                                               <  1:1551292/92‑1 (MQ=255)                                                    gagaGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCa                                                                                                                               >  2:1551292/1‑92 (MQ=255)                                                    gagaGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAgg                                                                                >  1:213384/1‑139 (MQ=255)                                                         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                                        <  1:1637943/94‑1 (MQ=255)                                                         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGAc                                                                                                                        >  2:1637943/1‑94 (MQ=255)                                                         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGcgcg                                                                           >  2:913532/1‑139 (MQ=255)                                                              cAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGcc                                                                      <  1:967238/139‑1 (MQ=255)                                                                                cggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCa                                                                                                                               <  2:1592672/64‑1 (MQ=255)                                                                                cggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCa                                                                                                                               >  1:1592672/1‑64 (MQ=255)                                                                                 ggTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCgg                                                   >  1:1396764/1‑139 (MQ=255)                                                                                   tGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGACAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGAt                                                 >  2:117586/1‑139 (MQ=255)                                                                                          cTTAACATCGTGCTGCTCCGCCTGCCGTCGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGAc                                          >  2:960490/1‑139 (MQ=255)                                                                                                aTCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAg                                    <  1:1433982/139‑1 (MQ=255)                                                                                                   gTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGa                                 <  1:913532/139‑1 (MQ=255)                                                                                                     gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                         <  2:1474740/49‑1 (MQ=255)                                                                                                     gctgctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAgaga                                                                                                                         >  1:1474740/1‑49 (MQ=255)                                                                                                        gctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGa                            <  1:254303/139‑1 (MQ=255)                                                                                                              ccTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTc                      >  2:1596177/1‑139 (MQ=255)                                                                                                                       ggATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGt             <  1:1875570/139‑1 (MQ=255)                                                                                                                             tttCAACGGCGCCCTGGCAAAGAGACAACTCATGCAtt                                                                                                            >  2:1661963/1‑38 (MQ=38)                                                                                                                             tttCAACGGCGCCCTGGCAAAGAGACAACTCATGCAtt                                                                                                            <  1:1661963/38‑1 (MQ=255)                                                                                                                             tttCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCg       >  1:1879619/1‑139 (MQ=255)                                                                                                                                  aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTg                                                                                               <  2:1166700/46‑1 (MQ=255)                                                                                                                                  aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTg                                                                                               >  1:1166700/1‑46 (MQ=255)                                                                                                                                  aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACATCAGTGAGCGACGCCCTGCGTGTGGTACATCCGGCTTCGTGCGTGGCGGCGAAAGGATCATTCGTGCGCAGTTATCCGTCAgg  >  1:880382/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                    CCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGG  >  NC_000913/3140158‑3140425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑