Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,178,015 G→A intergenic (‑105/‑100) nudF ← / → tolC ADP‑ribose pyrophosphatase/transport channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,178,0150GA100.0% 184.5 / NA 57intergenic (‑105/‑100)nudF/tolCADP‑ribose pyrophosphatase/transport channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/27);  total (30/27)

ATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGG  >  NC_000913/3177880‑3178143
                                                                                                                                       |                                                                                                                                
aTGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGacac                                                                                                                               >  1:1221161/1‑139 (MQ=255)
                 tCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTg                                                                                                              <  1:1720945/139‑1 (MQ=255)
                      cTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTa                                                                                                         >  1:1755246/1‑139 (MQ=255)
                          aGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCgg                                                                                                     >  1:1543796/1‑139 (MQ=255)
                          aGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCgg                                                                                                     >  1:316939/1‑139 (MQ=255)
                           gCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                    <  2:564066/139‑1 (MQ=255)
                                 ttAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGc                                                                                              >  1:973605/1‑139 (MQ=255)
                                           ggCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                    <  2:217636/139‑1 (MQ=255)
                                           ggCACTAATAGCGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTTGAATCCGc                                                                                    <  2:1221161/139‑1 (MQ=255)
                                                        aaTTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTg                                                                                                              <  2:693784/100‑1 (MQ=255)
                                                        aaTTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTg                                                                                                              >  1:693784/1‑100 (MQ=255)
                                                        aaTTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAg                                                                       >  2:1467837/1‑139 (MQ=255)
                                                          ttAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATc                                                                                       <  2:1705811/121‑1 (MQ=255)
                                                          ttAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATc                                                                                       >  1:1705811/1‑121 (MQ=255)
                                                             aaTGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                    <  1:1876324/105‑1 (MQ=255)
                                                             aaTGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                    >  2:1876324/1‑105 (MQ=255)
                                                                  gAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATcgcg                                                             <  1:1467837/139‑1 (MQ=255)
                                                                    aTTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                    >  1:919756/1‑98 (MQ=255)
                                                                    aTTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                    <  2:919756/98‑1 (MQ=255)
                                                                    aTTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCt                                                           >  1:1846565/1‑139 (MQ=255)
                                                                    aTTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAAGCCGCAATAATTTTATAGTTTGATCGCGCt                                                           >  2:575158/1‑139 (MQ=255)
                                                                          gCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                    <  1:1663675/92‑1 (MQ=255)
                                                                          gCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGc                                                                                                    >  2:1663675/1‑92 (MQ=255)
                                                                           cGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCa                                                                                                                      >  2:350859/1‑73 (MQ=255)
                                                                           cGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCa                                                                                                                      <  1:350859/73‑1 (MQ=255)
                                                                                tttGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCtt                                                >  1:978023/1‑138 (MQ=255)
                                                                                tttGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCtt                                                <  2:978023/138‑1 (MQ=255)
                                                                                tttGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTc                                               >  1:1787312/1‑139 (MQ=255)
                                                                                   gACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTcacc                                            <  2:1543796/139‑1 (MQ=255)
                                                                                        ccGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCg                                                                                                            >  1:1038049/1‑70 (MQ=255)
                                                                                        ccGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCg                                                                                                            <  2:1038049/70‑1 (MQ=255)
                                                                                        ccGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAg                                       >  2:1084262/1‑139 (MQ=255)
                                                                                                gCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCaa                               <  2:815341/139‑1 (MQ=255)
                                                                                                   gTCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATg                            >  1:251130/1‑139 (MQ=255)
                                                                                                    tCATGTGTTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATga                           >  2:1604078/1‑139 (MQ=255)
                                                                                                         tgtTAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGaaa                      <  2:1846565/139‑1 (MQ=255)
                                                                                                            tAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAg                                                                                                        <  1:853132/54‑1 (MQ=255)
                                                                                                            tAAATTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAg                                                                                                        >  2:853132/1‑54 (MQ=255)
                                                                                                              aaTTGAGGCACATTAACGCCCTATGACACGTAACGcc                                                                                                                       >  1:910268/1‑37 (MQ=255)
                                                                                                              aaTTGAGGCACATTAACGCCCTATGACACGTAACGcc                                                                                                                       <  2:910268/37‑1 (MQ=255)
                                                                                                              aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCt                                                                                                   >  1:427300/1‑57 (MQ=255)
                                                                                                              aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCt                                                                                                   <  2:427300/57‑1 (MQ=255)
                                                                                                              aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGAt                                                                 >  2:1614859/1‑91 (MQ=255)
                                                                                                              aaTTGAGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGAt                                                                 <  1:1614859/91‑1 (MQ=255)
                                                                                                                   aGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCaa                                                                                  >  1:44553/1‑69 (MQ=255)
                                                                                                                   aGGCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCaa                                                                                  <  2:44553/69‑1 (MQ=255)
                                                                                                                     gCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                    >  1:1009181/1‑65 (MQ=255)
                                                                                                                     gCACATTAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGc                                                                                    <  2:1009181/65‑1 (MQ=255)
                                                                                                                          ttAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATcgcg                                                             >  1:1122280/1‑83 (MQ=255)
                                                                                                                          ttAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATcgcg                                                             <  2:1122280/83‑1 (MQ=255)
                                                                                                                          ttAACGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGTTTCACCACAAGGAATTCAAATGAAGAAATTGCTCCCCATTCTTAt     <  1:1084262/139‑1 (MQ=255)
                                                                                                                             aCGCCCTATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCgg  >  2:1388531/1‑139 (MQ=255)
                                                                                                                                   tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCt                                                                                                   >  1:1548385/1‑36 (MQ=255)
                                                                                                                                   tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCt                                                                                                   <  2:1548385/36‑1 (MQ=255)
                                                                                                                                   tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCt                                                 <  2:425133/86‑1 (MQ=255)
                                                                                                                                   tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCt                                                 >  1:425133/1‑86 (MQ=255)
                                                                                                                                   tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTcacc                                            <  1:697020/91‑1 (MQ=255)
                                                                                                                                   tATGACACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTcacc                                            >  2:697020/1‑91 (MQ=255)
                                                                                                                                       |                                                                                                                                
ATGTAACGGGCAGGTTGTCTGGCTTAAGCATTGTTAATGTCCTGGCACTAATAGTGAATTAAATGTGAATTTCAGCGACGTTTGACTGCCGTTTGAGCAGTCATGTGTTAAATTGAGGCACATTAACGCCCTATGGCACGTAACGCCAACCTTTTGCGGTAGCGGCTTCTGCTAGAATCCGCAATAATTTTACAGTTTGATCGCGCTAAATACTGCTTCACCACAAGGAATGCAAATGAAGAAATTGCTCCCCATTCTTATCGG  >  NC_000913/3177880‑3178143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: