Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,282,504	A→G	T177A (ACC→GCC)	agaS →	tagatose‑6‑phosphate ketose/aldose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,282,504	0	A	G	100.0%	206.1 / NA	59	T177A (ACC→GCC)	agaS	tagatose‑6‑phosphate ketose/aldose isomerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/29);  total (30/29)

AATGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGTTACG  >  NC_000913/3282370‑3282639                                                                                                                                       |                                                                                                                                        aaTGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTgccac                                                                                                                                     >  2:330467/1‑139 (MQ=255)   tGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCGCGATCGCGGCTTTGCGATGACCAGCAGCATTGccacca                                                                                                                                   <  2:786415/139‑1 (MQ=255)              gCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATTGCCAGc                                                                                                                        >  2:1004422/1‑139 (MQ=255)              gCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGc                                                                                                                        >  1:1667963/1‑139 (MQ=255)                  aTCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGcc                                                                                                                    >  2:62309/1‑139 (MQ=255)                       cTGCAACGAAGCGGGCGCTCTTTACCAACACGCACTCAACAGCGATAACCCGTTTGCCCTTCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCTTGATGGCCAGCTGCCTcgcg                                                                                                               <  1:1004422/139‑1 (MQ=255)                         gCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGt                                                                                                             >  1:844425/1‑139 (MQ=255)                            aCGAAGCGGGCGCTCTTTACCAAAACGCGATGAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGAAGAAACGCACGATCGCGGCTTTGCGATAACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  1:501460/139‑1 (MQ=255)                            aCGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  1:486921/139‑1 (MQ=255)                               aaGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGc                                                                                                       >  2:1413819/1‑139 (MQ=255)                                   gggCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCt                                                                                                   <  2:1714097/139‑1 (MQ=255)                                    ggCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTACCTCGCGGTTTTCGCACCTg                                                                                                  >  2:1558391/1‑139 (MQ=255)                                        ctctTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGAc                                                                                              <  1:62309/139‑1 (MQ=255)                                            ttACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc                                                                                          <  2:580638/139‑1 (MQ=255)                                            ttACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc                                                                                          <  2:1314794/139‑1 (MQ=255)                                                   aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAg                                                                                                                         <  2:1470121/101‑1 (MQ=255)                                                   aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAg                                                                                                                         >  1:1470121/1‑101 (MQ=255)                                                   aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc                                                                                          >  1:800652/1‑132 (MQ=255)                                                   aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATc                                                                                          <  2:800652/132‑1 (MQ=255)                                                   aaCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACATcc                                                                                   >  1:1344277/1‑139 (MQ=255)                                                    aCGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTACCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCa                                                                                  <  1:1558391/139‑1 (MQ=255)                                                     cgcgATCAACAGCGATAACGCGTTTGTCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCaa                                                                                 >  2:686042/1‑139 (MQ=255)                                                     cgcgATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCaa                                                                                 >  2:1044826/1‑139 (MQ=255)                                                             aCAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCcg                                                                         <  1:1413819/139‑1 (MQ=255)                                                                gCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGa                                                                      >  1:1740824/1‑139 (MQ=255)                                                                    tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          >  2:785383/1‑99 (MQ=255)                                                                    tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  1:785383/99‑1 (MQ=255)                                                                    tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTg                                                                  >  1:12310/1‑139 (MQ=255)                                                                    tAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTCGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTg                                                                  >  2:1705707/1‑139 (MQ=255)                                                                      aCGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTcgcg                                                                                                               >  1:850139/1‑92 (MQ=255)                                                                      aCGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTcgcg                                                                                                               <  2:850139/92‑1 (MQ=255)                                                                      aCGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGc                                                                <  2:694203/139‑1 (MQ=255)                                                                           tttGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATc                                                           >  2:1296908/1‑139 (MQ=255)                                                                              gCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCg                                                                                                        >  1:1430596/1‑91 (MQ=255)                                                                              gCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCg                                                                                                        <  2:1430596/91‑1 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGc                                                                <  1:1666997/125‑1 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGc                                                                >  2:1666997/1‑125 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  <  1:1296908/139‑1 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  >  2:201963/1‑139 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  <  1:330467/139‑1 (MQ=255)                                                                                    ctgATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  <  2:1667963/139‑1 (MQ=255)                                                                                        tGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGt                                                                                                             <  1:1751034/76‑1 (MQ=255)                                                                                        tGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGt                                                                                                             >  2:1751034/1‑76 (MQ=255)                                                                                              cAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGAc                                        >  2:557790/1‑139 (MQ=255)                                                                                                   aCGCACGATCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCAc                                   >  1:1233978/1‑139 (MQ=255)                                                                                                           tCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  <  2:1565866/116‑1 (MQ=255)                                                                                                           tCGCGGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGACAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAgg                                                  >  1:1565866/1‑116 (MQ=255)                                                                                                              cgGCTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCC‑GGCGATCCTGACCTCACTGGGCGATTTCa                       >  1:455341/1‑139 (MQ=255)                                                                                                                 cTTTGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGc                     >  2:894867/1‑139 (MQ=255)                                                                                                                    tGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  2:1027445/51‑1 (MQ=255)                                                                                                                    tGCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          >  1:1027445/1‑51 (MQ=255)                                                                                                                     gCGATGACCAGGAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGAt                                              <  1:1182416/110‑1 (MQ=255)                                                                                                                     gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          >  2:1799213/1‑50 (MQ=255)                                                                                                                     gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGtttt                                                                                                          <  1:1799213/50‑1 (MQ=255)                                                                                                                     gCGATGACCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGAt                                              >  2:1182416/1‑110 (MQ=255)                                                                                                                           aCCAGCAGCATTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCC‑GGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGtt          <  2:455341/139‑1 (MQ=255)                                                                                                                                cagcaTTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCa                                                                                                      >  2:1690544/1‑43 (MQ=255)                                                                                                                                cagcaTTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCa                                                                                                      <  1:1690544/43‑1 (MQ=255)                                                                                                                                  gcaTTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGTTa    <  1:557790/139‑1 (MQ=255)                                                                                                                                    aTTGCCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGTTACg  <  1:686042/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AATGCTATCACCTGCCGATCACCTGCAACGAAGCGGGCGCTCTTTACCAAAACGCGATCAACAGCGATAACGCGTTTGCCCTGCTGATGCCCGCAGAAACGCACGATCGCGGCTTTGCGATGACCAGCAGCATTACCACCATGATGGCCAGCTGCCTCGCGGTTTTCGCACCTGAGACGATCAACAGCCAAACCTTCCGCGACGTGGCGGATCGTTGCCAGGCGATCCTGACCTCACTGGGCGATTTCAGCGAAGGTGTGTTTGGTTACG  >  NC_000913/3282370‑3282639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑