Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,299,438	A→G	T155T (ACA→ACG)	yhbO →	stress‑resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,438	0	A	G	100.0%	174.1 / NA	49	T155T (ACA→ACG)	yhbO	stress‑resistance protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/24);  total (25/24)

GATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGA  >  NC_000913/3299303‑3299563                                                                                                                                        |                                                                                                                              gATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            <  1:1665087/139‑1 (MQ=255)   tCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa                                                                                                                          <  1:22720/139‑1 (MQ=255)     aGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGccggacgccggacg                                                                                                                        <  2:46917/139‑1 (MQ=255)       cgcCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGAt                                                                                                                      >  1:1359296/1‑139 (MQ=255)                          cgcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                <  2:546836/126‑1 (MQ=255)                          cgcAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCa                                                                                                                >  1:546836/1‑126 (MQ=255)                                      gCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGcc                                                                                       <  2:1359296/139‑1 (MQ=255)                                                    cATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  <  2:293721/130‑1 (MQ=255)                                                    cATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTc                                                                                  >  1:293721/1‑130 (MQ=255)                                                              gTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGc                                                               >  2:1843346/1‑139 (MQ=255)                                                                        cGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAttt                                                     >  2:829177/1‑139 (MQ=255)                                                                         gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            >  1:995229/1‑67 (MQ=255)                                                                         gggCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCg                                                                                                                            <  2:995229/67‑1 (MQ=255)                                                                                 aaTTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCaa                                            <  1:203677/139‑1 (MQ=255)                                                                                        cGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGcc                                                                                       >  1:425824/1‑89 (MQ=255)                                                                                        cGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGcc                                                                                       <  2:425824/89‑1 (MQ=255)                                                                                           tCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCgt                                  >  1:999942/1‑139 (MQ=255)                                                                                             aGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa                                                                                                                          <  2:1724293/49‑1 (MQ=255)                                                                                             aGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGa                                                                                                                          >  1:1724293/1‑49 (MQ=255)                                                                                             aGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGc                                                                                                                  <  1:1032530/57‑1 (MQ=255)                                                                                             aGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGc                                                                                                                  >  2:1032530/1‑57 (MQ=255)                                                                                                     gTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGc                                                               <  2:749984/100‑1 (MQ=255)                                                                                                     gTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGc                                                               >  1:749984/1‑100 (MQ=255)                                                                                                     gTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTg                        >  2:329308/1‑139 (MQ=255)                                                                                                             tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGcc                                                                                                                 <  1:805217/42‑1 (MQ=255)                                                                                                             tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGcc                                                                                                                 >  2:805217/1‑42 (MQ=255)                                                                                                             tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTcgcg                                                                   >  2:144171/1‑88 (MQ=255)                                                                                                             tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTcgcg                                                                   <  1:144171/88‑1 (MQ=255)                                                                                                             tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATc                                                         >  2:507934/1‑98 (MQ=255)                                                                                                             tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATc                                                         <  1:507934/98‑1 (MQ=255)                                                                                                             tAAAGATCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGAt                >  1:946831/1‑139 (MQ=255)                                                                                                                   tCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAtttttt                                                  >  2:733852/1‑99 (MQ=255)                                                                                                                   tCAGCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAtttttt                                                  <  1:733852/99‑1 (MQ=255)                                                                                                                      gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                  >  2:1012244/1‑48 (MQ=255)                                                                                                                      gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                  >  2:1694586/1‑48 (MQ=255)                                                                                                                      gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                  <  1:1694586/48‑1 (MQ=255)                                                                                                                      gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGa                                                                                                  <  1:1012244/48‑1 (MQ=255)                                                                                                                      gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAg       <  2:999942/139‑1 (MQ=255)                                                                                                                      gCTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAg       >  2:807021/1‑139 (MQ=255)                                                                                                                       cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTAcc                                              <  2:1231843/99‑1 (MQ=255)                                                                                                                       cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGcc                                        >  1:718403/1‑105 (MQ=255)                                                                                                                       cTGGTTACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGcc                                        <  2:718403/105‑1 (MQ=255)                                                                                                                       cTGGTTACCAGACGGACGCCGGACGATCTGACAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTAcc                                              >  1:1231843/1‑99 (MQ=255)                                                                                                                           ttACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCt                                                                                   >  2:261343/1‑58 (MQ=255)                                                                                                                           ttACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCt                                                                                   <  1:261343/58‑1 (MQ=255)                                                                                                                            tACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAAt                     >  2:1011597/1‑119 (MQ=255)                                                                                                                            tACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAAt                     <  1:1011597/119‑1 (MQ=255)                                                                                                                            tACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGa  >  2:1466251/1‑138 (MQ=255)                                                                                                                            tACCAGCCGGACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGa  <  1:1466251/138‑1 (MQ=255)                                                                                                                                 gccggacgccggacGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGcc                                        <  2:204897/95‑1 (MQ=255)                                                                                                                                 gccggacgccggacGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGcc                                        >  1:204897/1‑95 (MQ=255)                                                                                                                                  ccggacgccggacgATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAt                                                       >  2:569668/1‑79 (MQ=255)                                                                                                                                  ccggacgccggacgATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCAt                                                       <  1:569668/79‑1 (MQ=255)                                                                                                                                     gACGCCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGcc                                        <  1:420780/91‑1 (MQ=255)                                                                                                                                        |                                                                                                                              GATCAGCGCCGATGTGATTCGGGGGCGCAAACTGACCGCAGTTAAACCGATCATTATTGATGTTAAAAATGCGGGCGCGGAATTTTACGATCAGGAAGTCGTGGTTGATAAAGATCAGCTGGTTACCAGCCGGACACCGGACGATCTGCCAGCGTTTAACCGCGAGGCGTTACGCCTGCTCGGTGCCTGAGTCGCGTAGCCAAATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGA  >  NC_000913/3299303‑3299563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑