Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,299,999	G→A	A12T (GCC→ACC)	yhbQ →	GIY‑YIG nuclease superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,299,999	0	G	A	100.0%	280.1 / NA	85	A12T (GCC→ACC)	yhbQ	GIY‑YIG nuclease superfamily protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/39);  total (46/39)

AACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATC  >  NC_000913/3299868‑3300134                                                                                                                                      |                                                                                                                                        aactgttggttTGGCAAGCAAACGGCTGATGGCGATGATGTTTTCCATTGC‑GTTCTCTTTTATTACTTTGGGCCTAACCTTAAAAGGCG‑CGGAGAACAATG‑CCCCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 <  1:257438/131‑1 (MQ=255) aacaTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACaa                                                                                                                                  >  2:421876/1‑139 (MQ=255) aacaTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACaa                                                                                                                                  >  1:1148207/1‑139 (MQ=255) aacaTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACaa                                                                                                                                  >  1:1696784/1‑139 (MQ=255)  acaTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 <  2:758844/139‑1 (MQ=255)   caTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATa                                                                                                                                >  1:1023001/1‑139 (MQ=255)   caTGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATa                                                                                                                                <  2:1501937/139‑1 (MQ=255)      gttgttTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGc                                                                                                                             >  1:66557/1‑139 (MQ=255)       ttgttTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                            >  2:209326/1‑139 (MQ=255)       ttgttTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                            <  2:827548/139‑1 (MQ=255)            ttGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTtata                                                                                                                       <  2:1023001/139‑1 (MQ=255)              gccagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATAcc                                                                                                                     >  2:341038/1‑139 (MQ=255)                cagccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCgg                                                                                                                   <  1:209326/139‑1 (MQ=255)                   ccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg                                                                                                                  >  2:1127041/1‑137 (MQ=255)                   ccaACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg                                                                                                                  <  1:1127041/137‑1 (MQ=255)                        ggCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTATTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcacca                                                                                                           <  2:1258377/139‑1 (MQ=255)                                        tgtTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 <  2:443462/101‑1 (MQ=255)                                        tgtTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 >  1:443462/1‑101 (MQ=255)                                          tttCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                            >  1:1297421/1‑104 (MQ=255)                                          tttCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                            <  2:1297421/104‑1 (MQ=255)                                                ttGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcagc                                                                                   <  1:421876/139‑1 (MQ=255)                                                  gCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcac                                                                                                             >  2:422402/1‑111 (MQ=255)                                                  gCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATcac                                                                                                             <  1:422402/111‑1 (MQ=255)                                                  gCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcagcag                                                                                 >  1:665127/1‑139 (MQ=255)                                                  gCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcagcag                                                                                 >  1:1526611/1‑139 (MQ=255)                                                      ttCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 <  1:1259563/87‑1 (MQ=255)                                                      ttCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 >  2:1259563/1‑87 (MQ=255)                                                             ttA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCgg                                                                      >  1:1532571/1‑139 (MQ=255)                                                                    tgtgGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg                                                                                                        <  2:426678/99‑1 (MQ=255)                                                                    tgtgGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg                                                                                                        >  1:426678/1‑99 (MQ=255)                                                                    tgtgGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgg                                                                <  2:1696784/139‑1 (MQ=255)                                                                    tgtgGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgg                                                                >  1:875937/1‑139 (MQ=255)                                                                      tgGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                              >  1:407673/1‑139 (MQ=255)                                                                      tgGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAgggg                                                              >  2:946879/1‑139 (MQ=255)                                                                        ggCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 >  1:1610584/1‑70 (MQ=255)                                                                        ggCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAAt                                                                                                                                 <  2:1610584/70‑1 (MQ=255)                                                                        ggCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCg                                                            >  2:1010017/1‑139 (MQ=255)                                                                         gCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGa                                                           >  1:892347/1‑139 (MQ=255)                                                                           cTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                            >  2:1513507/1‑72 (MQ=255)                                                                           cTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCt                                                                                                                            <  1:1513507/72‑1 (MQ=255)                                                                           cTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg                                                                                                                  >  2:1265406/1‑82 (MQ=255)                                                                           cTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCggg                                                                                                                  <  1:1265406/82‑1 (MQ=255)                                                                            tGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAg                                                        <  2:407673/139‑1 (MQ=255)                                                                              aCCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACa                                                                                                                                   >  2:153861/1‑62 (MQ=255)                                                                              aCCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACa                                                                                                                                   <  1:153861/62‑1 (MQ=255)                                                                              aCCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                                >  2:1844469/1‑97 (MQ=255)                                                                              aCCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGaa                                                                                                <  1:1844469/97‑1 (MQ=255)                                                                              aCCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCa                                                      <  2:1852969/139‑1 (MQ=255)                                                                              aCCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCa                                                      <  1:341038/139‑1 (MQ=255)                                                                              aCCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCa                                                      >  1:1852969/1‑139 (MQ=255)                                                                                 ttAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTg                                                   <  1:1010017/139‑1 (MQ=255)                                                                                   aaCATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                                    <  2:1298750/104‑1 (MQ=255)                                                                                   aaCATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                                    >  1:1298750/1‑104 (MQ=255)                                                                                        gCGACGCAG‑ACGATGACTCCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGa                                            >  2:1175191/1‑139 (MQ=255)                                                                                        gCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg                                                                                                        <  2:1073607/79‑1 (MQ=255)                                                                                        gCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg                                                                                                        >  1:1073607/1‑79 (MQ=255)                                                                                        gCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg                                                                                              <  1:841992/89‑1 (MQ=255)                                                                                        gCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAcg                                                                                              >  2:841992/1‑89 (MQ=255)                                                                                        gCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGC‑‑TCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaaa                                          >  1:1022484/1‑139 (MQ=255)                                                                                         cGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAg                                                        >  2:613206/1‑126 (MQ=255)                                                                                         cGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAg                                                        <  1:613206/126‑1 (MQ=255)                                                                                         cGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaa                                           >  1:1057484/1‑139 (MQ=255)                                                                                         cGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGaa                                           >  2:934378/1‑139 (MQ=255)                                                                                           aCGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa                                                                  <  1:1420892/114‑1 (MQ=255)                                                                                           aCGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa                                                                  >  2:1420892/1‑114 (MQ=255)                                                                                                       gACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACg                              <  2:1532571/139‑1 (MQ=255)                                                                                                       gACACCCTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACg                              <  2:892347/139‑1 (MQ=255)                                                                                                            ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa                                                                  <  1:655051/98‑1 (MQ=255)                                                                                                            ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa                                                                  >  2:655051/1‑98 (MQ=255)                                                                                                            ccTGGTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt                         >  1:1590878/1‑139 (MQ=255)                                                                                                                gTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                                    <  1:925875/76‑1 (MQ=255)                                                                                                                gTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATcag                                                                                    >  2:925875/1‑76 (MQ=255)                                                                                                                gTTTCTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGttt                     >  2:1853729/1‑139 (MQ=255)                                                                                                                    cTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt                         >  2:1096658/1‑131 (MQ=255)                                                                                                                    cTTTACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGgt                         <  1:1096658/131‑1 (MQ=255)                                                                                                                     tttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCcgc                >  1:1469199/1‑139 (MQ=255)                                                                                                                      ttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg                                                                                                        >  2:1141914/1‑50 (MQ=255)                                                                                                                      ttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACgg                                                                                                        <  1:1141914/50‑1 (MQ=255)                                                                                                                      ttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAc                                                                                               <  1:53985/59‑1 (MQ=255)                                                                                                                      ttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAAc                                                                                               >  2:53985/1‑59 (MQ=255)                                                                                                                      ttACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCcgcg               <  2:665127/139‑1 (MQ=255)                                                                                                                       tACTTGATCCGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCgcgc              <  2:1526611/139‑1 (MQ=255)                                                                                                                               ccGTACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGc      >  2:962395/1‑139 (MQ=255)                                                                                                                                  tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa                                                                  <  1:1621653/76‑1 (MQ=255)                                                                                                                                  tACCACCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCaaa                                                                  >  2:1621653/1‑76 (MQ=255)                                                                                                                                   accaccGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAAc                                   <  2:1544855/106‑1 (MQ=255)                                                                                                                                   accaccGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATc  >  2:1882864/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                        AACATGTTGTTTTGCCAGCCAACGGCTGATGGCGATGAGTGTTTCCATTGCTGTTCTCTTTTA‑TACTGTGGGCCTGACCTTAACATGCGACGCAG‑ACGATGACACCCTGGTTTCTTTACTTGATCCGTACCGCCGACAATAAGCTTTATACCGGGATCACCACGGATGTCGAACGCCGCTATCAGCAGCACCAAAGCGGCAAAGGGGCGAAAGCACTGCGCGGGAAAGGAGAACTAACGCTGGTGTTTTCCGCGCCAGTGGGCGATC  >  NC_000913/3299868‑3300134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑