Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,343,891 A→G D171G (GAC→GGC)  lptA → lipopolysaccharide export ABC transporter periplasmic binding protein, Lipid A binding protein, LPS export and assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,343,8910AG100.0% 309.2 / NA 86D171G (GAC→GGC) lptAlipopolysaccharide export ABC transporter periplasmic binding protein, Lipid A binding protein, LPS export and assembly protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/43);  total (43/43)

GCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGA  >  NC_000913/3343758‑3344016
                                                                                                                                     |                                                                                                                             
gCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCaaaa                                                                                                                          <  2:299680/139‑1 (MQ=255)
 cTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCaaaaa                                                                                                                         >  2:1337513/1‑139 (MQ=255)
     tCTGCAGCAGGTCGATGGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAaacaac                                                                                                                     >  2:1711859/1‑139 (MQ=255)
         cagcagGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAg                                                                                                                 >  1:1095711/1‑139 (MQ=255)
               gTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGa                                                                                                           >  1:284690/1‑139 (MQ=255)
               gTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGa                                                                                                           >  2:1703935/1‑139 (MQ=255)
                  gATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        <  1:1703935/139‑1 (MQ=255)
                  gATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        <  2:623405/139‑1 (MQ=255)
                  gATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        <  1:889855/139‑1 (MQ=255)
                       cAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   <  1:265688/139‑1 (MQ=255)
                       cAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   <  1:52366/139‑1 (MQ=255)
                         aCATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGcaca                                                                                                 >  1:1584944/1‑139 (MQ=255)
                                    gATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAacaaca                                                                                                                    <  1:61779/109‑1 (MQ=255)
                                    gATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAacaaca                                                                                                                    >  2:61779/1‑109 (MQ=255)
                                     aTAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGa                                                                                                           >  2:174182/1‑117 (MQ=255)
                                     aTAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGa                                                                                                           <  1:174182/117‑1 (MQ=255)
                                     aTAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGtaa                                                                                     >  2:734441/1‑139 (MQ=255)
                                           tCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTaattaatt                                                                               >  2:125662/1‑139 (MQ=255)
                                              cTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        <  2:1012901/111‑1 (MQ=255)
                                              cTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        >  1:1012901/1‑111 (MQ=255)
                                              cTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGt                                                                            <  1:340662/139‑1 (MQ=255)
                                                tACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCaa                                                                                                                            >  1:1552520/1‑89 (MQ=255)
                                                tACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCaa                                                                                                                            <  2:1552520/89‑1 (MQ=255)
                                                   cTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAacaaca                                                                                                                    <  2:1416275/94‑1 (MQ=255)
                                                   cTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAacaaca                                                                                                                    >  1:1416275/1‑94 (MQ=255)
                                                   cTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATgg                                                                       >  1:1807612/1‑139 (MQ=255)
                                                   cTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATgg                                                                       <  2:347204/139‑1 (MQ=255)
                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGc                                                                                                               >  1:1631837/1‑98 (MQ=255)
                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGc                                                                                                               <  2:1631837/98‑1 (MQ=255)
                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   >  2:1511631/1‑110 (MQ=255)
                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   >  1:950301/1‑110 (MQ=255)
                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   <  2:950301/110‑1 (MQ=255)
                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   <  1:1511631/110‑1 (MQ=255)
                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGt                                                                            >  2:89070/1‑133 (MQ=255)
                                                    tGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGt                                                                            <  1:89070/133‑1 (MQ=255)
                                                      gTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGt                                                                                       <  2:1129075/120‑1 (MQ=255)
                                                      gTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGt                                                                                       >  1:1129075/1‑120 (MQ=255)
                                                      gTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCaa                                                                    >  1:1612822/1‑139 (MQ=255)
                                                       tGAAAGAGCAGAAAATGTAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   <  2:494399/107‑1 (MQ=255)
                                                       tGAAAGAGCAGAAAATGTAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   >  1:494399/1‑107 (MQ=255)
                                                       tGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGc                                                                                                               >  1:753089/1‑95 (MQ=255)
                                                       tGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGc                                                                                                               <  2:753089/95‑1 (MQ=255)
                                                        gAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACa                                                                  >  1:490630/1‑139 (MQ=255)
                                                           agagCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCaaaaa                                                                                                                         <  1:1020222/81‑1 (MQ=255)
                                                           agagCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCaaaaa                                                                                                                         >  2:1020222/1‑81 (MQ=255)
                                                           agagCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTa                                                               >  2:402886/1‑139 (MQ=255)
                                                            gagCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTaa                                                              <  1:1337513/139‑1 (MQ=255)
                                                            gagCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTaa                                                              <  1:402886/139‑1 (MQ=255)
                                                               cAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTg                                                           >  1:780637/1‑139 (MQ=255)
                                                                aGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   <  2:1063641/98‑1 (MQ=255)
                                                                aGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   >  1:1063641/1‑98 (MQ=255)
                                                                         aGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        <  2:291163/84‑1 (MQ=255)
                                                                         aGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        >  1:291163/1‑84 (MQ=255)
                                                                             tttCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCa                                             <  1:714825/139‑1 (MQ=255)
                                                                             tttCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCa                                             <  2:1095711/139‑1 (MQ=255)
                                                                              ttCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCGGACCCCGTCACAGAAGATGAGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaa                                            <  1:1209226/139‑1 (MQ=255)
                                                                              ttCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaa                                            <  2:780637/139‑1 (MQ=255)
                                                                               tCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaaa                                           <  1:734441/139‑1 (MQ=255)
                                                                                     aCAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAacaaca                                                                                                                    <  2:1230170/60‑1 (MQ=255)
                                                                                     aCAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAacaaca                                                                                                                    >  1:1230170/1‑60 (MQ=255)
                                                                                     aCAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGaagaag                                                                                          <  2:1499715/86‑1 (MQ=255)
                                                                                     aCAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGaagaag                                                                                          >  1:1499715/1‑86 (MQ=255)
                                                                                       aaaGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   >  1:521016/1‑75 (MQ=255)
                                                                                       aaaGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   <  2:521016/75‑1 (MQ=255)
                                                                                       aaaGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCtata                                   >  1:792438/1‑139 (MQ=255)
                                                                                             aaGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAacaaca                                                                                                                    >  2:1540073/1‑52 (MQ=255)
                                                                                             aaGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAacaaca                                                                                                                    <  1:1540073/52‑1 (MQ=255)
                                                                                               gcgcGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGt                           >  2:196875/1‑139 (MQ=255)
                                                                                                cgcgTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGc                                                                                                               <  1:656806/54‑1 (MQ=255)
                                                                                                cgcgTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGc                                                                                                               >  2:656806/1‑54 (MQ=255)
                                                                                                cgcgTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        <  2:55112/61‑1 (MQ=255)
                                                                                                cgcgTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        >  1:55112/1‑61 (MQ=255)
                                                                                                 gcgTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGTAAAGAACCTTGCAAAAGCCTATAAAGGCCGTcg                         <  2:284690/139‑1 (MQ=255)
                                                                                                   gTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTcgcg                       <  2:1807612/139‑1 (MQ=255)
                                                                                                             ttCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaaa                                           >  1:1065976/1‑109 (MQ=255)
                                                                                                             ttCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCaaa                                           <  2:1065976/109‑1 (MQ=255)
                                                                                                             ttCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTagaaga             >  2:1298850/1‑139 (MQ=255)
                                                                                                              tCTGGTGCCGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGAc            >  2:1072491/1‑139 (MQ=255)
                                                                                                                     ccGTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGcc     <  2:792438/139‑1 (MQ=255)
                                                                                                                       gTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   >  1:861733/1‑43 (MQ=255)
                                                                                                                       gTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGca                                                                                                   <  2:861733/43‑1 (MQ=255)
                                                                                                                       gTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGc                                                                      >  1:930081/1‑72 (MQ=255)
                                                                                                                       gTCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGc                                                                      <  2:930081/72‑1 (MQ=255)
                                                                                                                        tCGCAGCTGCAGGGCAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGa  >  2:488173/1‑139 (MQ=255)
                                                                                                                         cGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        >  1:1171016/1‑36 (MQ=255)
                                                                                                                         cGCAGCTGCAGGGCAAAAACAACAAAGGCCAGAccc                                                                                                        <  2:1171016/36‑1 (MQ=255)
                                                                                                                                     |                                                                                                                             
GCTTATCTGCAGCAGGTCGATAGCAACATTAAGGGCGATAAGATCACTTACCTGGTGAAAGAGCAGAAAATGCAGGCTTTCAGCGACAAAGGCAAGCGCGTAACAACCGTTCTGGTGCCGTCGCAGCTGCAGGACAAAAACAACAAAGGCCAGACCCCGGCACAGAAGAAGGGTAATTAATTCGTTATGGCAACATTAACTGCAAAGAACCTTGCAAAAGCCTATAAAGGCCGTCGCGTGGTAGAAGACGTCAGCCTGA  >  NC_000913/3343758‑3344016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: