Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,472,033	G→A	I494I (ATC→ATT)	fusA ←	protein chain elongation factor EF‑G, GTP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,472,033	0	G	A	100.0%	234.7 / NA	72	I494I (ATC→ATT)	fusA	protein chain elongation factor EF‑G, GTP‑binding
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/38);  total (34/38)

GTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGGATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTC  >  NC_000913/3471901‑3472167                                                                                                                                     |                                                                                                                                       gTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGtt                                                                                                                                  >  1:1843145/1‑139 (MQ=255) gTTGATAAACTCGTAGCCTTTCGGGTTTAAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGtt                                                                                                                                  >  1:491911/1‑139 (MQ=255)                gCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAAcc                                                                                                                   <  1:1832601/139‑1 (MQ=255)                  cTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                                 <  2:1358172/139‑1 (MQ=255)                  cTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                                 <  2:348362/139‑1 (MQ=255)                  cTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTg                                                                                                                 <  1:984964/139‑1 (MQ=255)                            tGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCt                                                                                                       >  2:1165736/1‑139 (MQ=255)                                   ggCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCg                                                                                                >  2:467121/1‑139 (MQ=255)                                    gCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGc                                                                                               >  2:1093039/1‑139 (MQ=255)                                           cGGGTACATGTCGATAACAACATGACCATACTGACGACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                              <  1:1281117/101‑1 (MQ=255)                                           cGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCgg                                                                                                              >  2:7466/1‑117 (MQ=255)                                           cGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCgg                                                                                                              <  1:7466/117‑1 (MQ=255)                                           cGGGTACATGTCGATAACAACACGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCCACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                              >  2:1281117/1‑101 (MQ=255)                                               tACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGa                                                                                    >  1:932213/1‑139 (MQ=255)                                                  aTGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAAtt                                                                                 <  1:75203/139‑1 (MQ=255)                                                      cGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACg                                                                             <  1:1453843/139‑1 (MQ=255)                                                      cGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACg                                                                             <  1:1165736/139‑1 (MQ=255)                                                       gATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGc                                                                            >  2:537073/1‑139 (MQ=255)                                                             aacaTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATa                                                                      <  1:624959/139‑1 (MQ=255)                                                               caTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCa                                                                               >  2:90278/1‑128 (MQ=255)                                                               caTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCa                                                                               <  1:90278/128‑1 (MQ=255)                                                               caTGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    <  2:491911/139‑1 (MQ=255)                                                                       tACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCAt                                                                       >  1:388420/1‑128 (MQ=255)                                                                       tACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCAt                                                                       <  2:388420/128‑1 (MQ=255)                                                                         cTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACgatg                                                          <  2:1080670/139‑1 (MQ=255)                                                                            accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    >  1:1650301/1‑126 (MQ=255)                                                                            accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    >  1:106990/1‑126 (MQ=255)                                                                            accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    <  2:106990/126‑1 (MQ=255)                                                                            accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACg                                                                    <  2:1650301/126‑1 (MQ=255)                                                                            accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGatgatg                                                       >  1:1067092/1‑139 (MQ=255)                                                                            accacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGatgatg                                                       >  1:933210/1‑139 (MQ=255)                                                                              cacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTc                                                     >  1:1798478/1‑139 (MQ=255)                                                                              cacgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTc                                                     >  1:1143505/1‑139 (MQ=255)                                                                               acgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                              <  2:214339/65‑1 (MQ=255)                                                                               acgaccacCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCAc                                                                                                                              >  1:214339/1‑65 (MQ=255)                                                                                  accaccAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAgg                                                 <  2:1192877/139‑1 (MQ=255)                                                                                      ccaGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCa                                             >  2:1765148/1‑139 (MQ=255)                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTAc                                                                                                         >  1:39938/1‑71 (MQ=255)                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTAc                                                                                                         <  2:39938/71‑1 (MQ=255)                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTc                                                                                <  2:1297839/96‑1 (MQ=255)                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTc                                                                                >  1:1297839/1‑96 (MQ=255)                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGt                                                <  1:546859/128‑1 (MQ=255)                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGt                                                >  2:546859/1‑128 (MQ=255)                                                                                              tttCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGccc                                     >  1:1357314/1‑139 (MQ=255)                                                                                                   cgTGTTTACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACATGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAcc                                <  2:998179/139‑1 (MQ=255)                                                                                                       tttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGt                                           >  2:1067746/1‑124 (MQ=255)                                                                                                       tttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGt                                           <  1:1067746/124‑1 (MQ=255)                                                                                                        ttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAcc                                <  2:1423308/134‑1 (MQ=255)                                                                                                        ttACCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATAcc                                >  1:1423308/1‑134 (MQ=255)                                                                                                          aCCTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCgatg                         <  2:1067092/139‑1 (MQ=255)                                                                                                          aCCTTCAACATCGGTAACTTTATGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGTgatg                         <  1:1093039/139‑1 (MQ=255)                                                                                                           ccTTCAACATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCgatga                        <  2:933210/139‑1 (MQ=255)                                                                                                                aaCATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCGACCTGCGGTTTACCTACGTTCGCTTCGACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTc                   <  2:1143505/139‑1 (MQ=255)                                                                                                                aaCATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              <  2:1305697/128‑1 (MQ=255)                                                                                                                aaCATCGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              >  1:1305697/1‑128 (MQ=255)                                                                                                                     cGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              <  2:284768/123‑1 (MQ=255)                                                                                                                     cGGTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              >  1:284768/1‑123 (MQ=255)                                                                                                                      ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              <  1:1855177/122‑1 (MQ=255)                                                                                                                      ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCcg                              >  2:1855177/1‑122 (MQ=255)                                                                                                                      ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATgg                     <  1:675594/131‑1 (MQ=255)                                                                                                                      ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATgg                     >  2:675594/1‑131 (MQ=255)                                                                                                                      ggTAACTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTa             >  1:385762/1‑139 (MQ=255)                                                                                                                           cTTTCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGAttc        <  2:1357314/139‑1 (MQ=255)                                                                                                                             ttCTGGCGAATAGTTTCACGATAAGAAACCTGCGGATTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTCGTTAGAttctt      >  1:185004/1‑139 (MQ=255)                                                                                                                              tCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCa                                                                >  2:901308/1‑80 (MQ=255)                                                                                                                              tCTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCa                                                                <  1:901308/80‑1 (MQ=255)                                                                                                                               cTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCg    <  1:467121/139‑1 (MQ=255)                                                                                                                               cTGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCg    <  1:537073/139‑1 (MQ=255)                                                                                                                                tGGCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGt   >  2:1449121/1‑139 (MQ=255)                                                                                                                                 ggCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTc  <  1:569031/139‑1 (MQ=255)                                                                                                                                  gCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGCATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTc                   >  1:553224/1‑121 (MQ=255)                                                                                                                                  gCGAATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCGTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTc                   <  2:553224/121‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GTTGATGAACTCGTAGCCTTTCGGGTTTGAACCCGGCTCCAGCGGGTACATGTCGATAACAACATGACCATACTGACCACGACCACCAGACTGTTTCGCGTGTTTACCTTCAACATCGGTAACTTTCTGGCGGATAGTTTCACGGTAAGCAACCTGCGGTTTACCTACGTTCGCTTCAACGTTGAATTCACGCTTCATACGGTCAACGATGATGTCGAGGTGCAGTTCGCCCATACCCGCGATGATGGTCTGGTTAGATTCTTCGTC  >  NC_000913/3471901‑3472167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑