Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,719,768 G→A *97* (TAG→TAA)  yiaG → HTH_CROC1 family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,719,7680GA98.3% 184.7 / NA 58*97* (TAG→TAA) yiaGHTH_CROC1 family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (26/31);  minor base T (0/1);  total (26/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTT  >  NC_000913/3719639‑3719895
                                                                                                                                 |                                                                                                                               
ggTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                        <  2:1648893/139‑1 (MQ=255)
        gCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTAt                                                                                                                <  2:1183649/139‑1 (MQ=255)
            aTCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCt                                                                                                            <  2:584717/139‑1 (MQ=255)
                 tCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                       <  1:3065/139‑1 (MQ=255)
                      aTGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATATACTTTTATCCACTTTATTg                                                                                                              <  2:1252917/127‑1 (MQ=255)
                      aTGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTg                                                                                                              >  1:1252917/1‑127 (MQ=255)
                                  tGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                       <  2:567990/122‑1 (MQ=255)
                                  tGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                       >  1:567990/1‑122 (MQ=255)
                                  tGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGa                                                                                      >  1:766121/1‑139 (MQ=255)
                                    ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                       <  1:1622822/120‑1 (MQ=255)
                                    ggAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                       >  2:1622822/1‑120 (MQ=255)
                                        tCCAGACGCGTGAAGCCTTCAAGTTCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                       <  2:1120343/116‑1 (MQ=255)
                                        tCCAGACGCGTGAAGCCTTCAAGTGCCTAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTa                                                                                                       >  1:1120343/1‑116 (MQ=255)
                                        tCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGttt                                                                                >  1:1433146/1‑139 (MQ=255)
                                        tCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAAACCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGGTGTTTACGGTCCTGATGACAGGACCGttt                                                                                >  1:1089832/1‑139 (MQ=255)
                                            gACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCa                                                                            <  1:1851711/139‑1 (MQ=255)
                                              cgcgTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAAc                                                                          <  2:1433146/139‑1 (MQ=255)
                                              cgcgTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAAc                                                                          <  2:766121/139‑1 (MQ=255)
                                               gcgTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCaa                                                                           <  2:845633/137‑1 (MQ=255)
                                               gcgTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCaa                                                                           >  1:845633/1‑137 (MQ=255)
                                                 gTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                       <  2:490031/139‑1 (MQ=255)
                                                 gTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                       <  2:278236/139‑1 (MQ=255)
                                                    aaGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCATGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTa                                                                    >  1:1159047/1‑139 (MQ=255)
                                                         ttCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCAt                                                               >  2:536423/1‑139 (MQ=255)
                                                           cAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                        >  1:988245/1‑80 (MQ=255)
                                                           cAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                        <  2:988245/80‑1 (MQ=255)
                                                                 ccGAACTAAAATTGATGCGGTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGa                                                       >  1:1038055/1‑139 (MQ=255)
                                                                  cGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACg                                                                                                     <  1:81222/92‑1 (MQ=255)
                                                                  cGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACg                                                                                                     >  2:81222/1‑92 (MQ=255)
                                                                    aaCTAAAATTGATGCGTTTGATTCAAGTCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                       >  1:1866848/1‑120 (MQ=255)
                                                                    aaCTAAAATTGATGCGTTTGATTCAAGTCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                       <  2:1866848/120‑1 (MQ=255)
                                                                    aaCTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGaaaa                                                    >  2:664915/1‑139 (MQ=255)
                                                                               aTGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                        <  1:461299/60‑1 (MQ=255)
                                                                               aTGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                        >  2:461299/1‑60 (MQ=255)
                                                                                tGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                       >  1:19691/1‑108 (MQ=255)
                                                                                tGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGa                                                                       <  2:19691/108‑1 (MQ=255)
                                                                                tGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCa                                        <  1:536423/139‑1 (MQ=255)
                                                                                 gCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCAt                                       >  1:1279006/1‑139 (MQ=255)
                                                                                    tttGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCAc                                    <  1:721066/139‑1 (MQ=255)
                                                                                     ttGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCAcc                                   <  2:1089832/139‑1 (MQ=255)
                                                                                       gATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCg                                 <  2:1093020/139‑1 (MQ=255)
                                                                                         ttCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGcc                               <  1:664915/139‑1 (MQ=255)
                                                                                          tCAAGCCAACCCGGGATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTCTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCa                              <  2:1159047/139‑1 (MQ=255)
                                                                                              gCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                        >  2:863737/1‑45 (MQ=255)
                                                                                              gCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATc                                                                                                                        <  1:863737/45‑1 (MQ=255)
                                                                                                   cccGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACg                                                                                                     >  2:1031451/1‑59 (MQ=255)
                                                                                                   cccGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACg                                                                                                     <  1:1031451/59‑1 (MQ=255)
                                                                                                   cccGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                      <  1:88966/90‑1 (MQ=255)
                                                                                                   cccGGCATTAAGTAAGCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGAt                                                                      >  2:88966/1‑90 (MQ=255)
                                                                                                            aagtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCt                                                                                                >  1:657395/1‑55 (MQ=255)
                                                                                                            aagtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCt                                                                                                <  2:657395/55‑1 (MQ=255)
                                                                                                             agtaagCAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGcaca           >  2:1776854/1‑139 (MQ=255)
                                                                                                                   cAGTTGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACg     <  2:1038055/139‑1 (MQ=255)
                                                                                                                      ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTa                                                                    >  1:1524152/1‑73 (MQ=255)
                                                                                                                      ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTa                                                                    <  2:1524152/73‑1 (MQ=255)
                                                                                                                      ttGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGtt  >  2:428374/1‑139 (MQ=255)
                                                                                                                       tGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGt   >  1:967383/1‑137 (MQ=255)
                                                                                                                       tGATGGAATAAACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGt   <  2:967383/137‑1 (MQ=255)
                                                                                                                                 |                                                                                                                               
GGTTTTGGGCGTATCAGTCGCCATGGTAAAGGAATGGGAATCCAGACGCGTGAAGCCTTCAAGTGCCGAACTAAAATTGATGCGTTTGATTCAAGCCAACCCGGCATTAAGTAAGCAGTTGATGGAATAGACTTTTATCCACTTTATTGCTGTTTACGGTCCTGATGACAGGACCGTTTTCCAACCGATTAATCATAAATATGAAAAATAATTGTTGCATCACCCGCCAATGCGTGGCTTAATGCACATCAACGGTT  >  NC_000913/3719639‑3719895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑