Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,750,092 G→A *287* (TAG→TAA) 
E3K (GAG→AAG) 		 sgbU →
sgbE →		 putative L‑xylulose 5‑phosphate 3‑epimerase
L‑ribulose‑5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,750,0920GA100.0% 192.5 / NA 59*287* (TAG→TAA) 
E3K (GAG→AAG) 		sgbU
sgbE		putative L‑xylulose 5‑phosphate 3‑epimerase
L‑ribulose‑5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/27);  total (32/27)

CGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGT  >  NC_000913/3749956‑3750227
                                                                                                                                        |                                                                                                                                       
cGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTaaag                                                                                                                                       <  1:1082568/139‑1 (MQ=255)
   tGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCaa                                                                                                                                    >  1:1193597/1‑139 (MQ=255)
     cATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACt                                                                                                                                  >  1:660723/1‑139 (MQ=255)
             gAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCg                                                                                                                          >  1:1028227/1‑139 (MQ=255)
             gAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCg                                                                                                                          >  1:1314788/1‑139 (MQ=255)
                   tCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGc                                                                                                                    >  2:1023324/1‑139 (MQ=255)
                    cgcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCt                                                                                                                   >  2:1063636/1‑139 (MQ=255)
                     gcgGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCAGGAGATTATTCAGGCGCGGCGTTCGATTGAAGCGCGTATGCAGGAGGCTGCATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTg                                                                                                                  <  2:1193597/139‑1 (MQ=255)
                             ttCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGaa                                                                                                          >  1:211981/1‑139 (MQ=255)
                                      gagaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCt                                                                                                 >  1:419616/1‑139 (MQ=255)
                                        gaTGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGATAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                               >  1:455785/1‑139 (MQ=255)
                                             ggACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCt                                                                                          <  1:1063636/139‑1 (MQ=255)
                                              gACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTc                                                                                         >  2:587588/1‑139 (MQ=255)
                                               aCCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                        <  2:455785/139‑1 (MQ=255)
                                               aCCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                        <  1:1728556/139‑1 (MQ=255)
                                               aCCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                        <  2:365927/139‑1 (MQ=255)
                                                 cGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCAcc                                                                                      <  2:1314788/139‑1 (MQ=255)
                                                      aaGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTg                                                                                 >  1:619377/1‑139 (MQ=255)
                                                              gagCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTccc                                                                                             <  2:401236/119‑1 (MQ=255)
                                                              gagCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTccc                                                                                             >  1:401236/1‑119 (MQ=255)
                                                                 ccGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCAc                                                                      <  2:419616/139‑1 (MQ=255)
                                                                         ggAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                               <  2:1871786/106‑1 (MQ=255)
                                                                         ggAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTc                                                                                               >  1:1871786/1‑106 (MQ=255)
                                                                               tattCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCa                                                        <  1:1023324/139‑1 (MQ=255)
                                                                                attCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAg                                                       <  2:660723/139‑1 (MQ=255)
                                                                                 ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCg                                                                                            <  1:1116930/101‑1 (MQ=255)
                                                                                 ttCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACAGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCg                                                                                            >  2:1116930/1‑101 (MQ=255)
                                                                                   cAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAgcgc                                                    <  2:211981/139‑1 (MQ=255)
                                                                                         cggcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                        >  1:614167/1‑97 (MQ=255)
                                                                                         cggcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCa                                                                                        <  2:614167/97‑1 (MQ=255)
                                                                                         cggcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGa                                              >  2:671212/1‑139 (MQ=255)
                                                                                         cggcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGa                                              >  1:131381/1‑139 (MQ=255)
                                                                                           gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGc                                                                                                                                      >  2:55825/1‑49 (MQ=255)
                                                                                           gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGc                                                                                                                                      <  1:55825/49‑1 (MQ=255)
                                                                                           gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGaa                                                                                                                               >  1:274316/1‑56 (MQ=255)
                                                                                           gcgTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGaa                                                                                                                               <  2:274316/56‑1 (MQ=255)
                                                                                                ggATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAAcg                                       <  1:587588/139‑1 (MQ=255)
                                                                                                     gAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCa                                  <  2:619377/139‑1 (MQ=255)
                                                                                                           cgTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACg                                                                           >  1:237878/1‑92 (MQ=255)
                                                                                                           cgTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACg                                                                           <  2:237878/92‑1 (MQ=255)
                                                                                                           cgTATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAatggat                            >  1:1356600/1‑139 (MQ=255)
                                                                                                             tATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAAcg                                       <  1:500680/126‑1 (MQ=255)
                                                                                                             tATGCAGGAGGCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAAcg                                       >  2:500680/1‑126 (MQ=255)
                                                                                                                   ggaggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGcgcg                                                   <  2:384434/108‑1 (MQ=255)
                                                                                                                   ggaggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGcgcg                                                   >  1:384434/1‑108 (MQ=255)
                                                                                                                    gaggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTgggg                                                                <  2:605636/94‑1 (MQ=255)
                                                                                                                    gaggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTgggg                                                                >  1:605636/1‑94 (MQ=255)
                                                                                                                    gaggCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCaa                   >  1:739572/1‑139 (MQ=255)
                                                                                                                       gCTGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAAcc                >  1:1444291/1‑139 (MQ=255)
                                                                                                                         tGGATTTATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGACTCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCtt              >  1:685100/1‑139 (MQ=255)
                                                                                                                              ttATATGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGc         >  1:121698/1‑139 (MQ=255)
                                                                                                                                atatGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGt       <  2:1356600/139‑1 (MQ=255)
                                                                                                                                atatGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGt       <  1:671212/139‑1 (MQ=255)
                                                                                                                                 tatGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTc      >  2:1699882/1‑139 (MQ=255)
                                                                                                                                 tatGTTAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTc      >  2:840418/1‑139 (MQ=255)
                                                                                                                                     ttAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACg                                                                           <  2:1882201/66‑1 (MQ=255)
                                                                                                                                     ttAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACg                                                                           >  1:1882201/1‑66 (MQ=255)
                                                                                                                                     ttAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCt                                                                    >  1:1149046/1‑73 (MQ=255)
                                                                                                                                     ttAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGt  <  2:1028227/139‑1 (MQ=255)
                                                                                                                                     ttAAAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACATTCACCt                                                                    <  2:1149046/73‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CGCTGCATAAACTGAACTATCGCGGTTCTTTCCTGATTGAGATGTGGACCGAAAAAGCCAAAGAGCCGGTGCTGGAGATTATTCAGGCGCGGCGTTGGATTGAAGCGCGTATGCAGGAGGCTGGATTTATATGTTAGAGCAACTGAAAGCCGACGTGCTGGCGGCGAATCTGGCGCTTCCCGCTCACCATCTGGTGACGTTCACCTGGGGCAATGTCAGCGCGGTAGACGAAACGCGGCAATGGATGGTAATCAAACCTTCCGGCGTCGAGT  >  NC_000913/3749956‑3750227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑