Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,757,183	A→G	C215C (TGT→TGC)	yiaY ←	L‑threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,757,183	0	A	G	100.0%	270.3 / NA	77	C215C (TGT→TGC)	yiaY	L‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/41);  total (36/41)

AACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAAG  >  NC_000913/3757047‑3757314                                                                                                                                         |                                                                                                                                    aaCCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGcgca                                                                                                                                   >  1:840955/1‑139 (MQ=255) aaCCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGcgca                                                                                                                                   >  1:966985/1‑139 (MQ=255) aaCCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGcgca                                                                                                                                   <  1:1565119/139‑1 (MQ=255)   cccAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAg                                                                                                                                 <  2:840955/139‑1 (MQ=255)   cccAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAg                                                                                                                                 <  2:624263/139‑1 (MQ=255)      agagAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGt                                                                                                                              <  2:1076692/139‑1 (MQ=255)        agaAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCa                                                                                                                            >  2:1653551/1‑139 (MQ=255)           aGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTg                                                                                                                         >  1:1445744/1‑139 (MQ=255)             cATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGAt                                                                                                                       >  1:1628760/1‑139 (MQ=255)             cATTATTGAACGCCATACCGGCGAGGAACT‑GGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATc                                                                                                                      >  1:1418665/1‑139 (MQ=255)                        gCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcgg                                                                                                            <  1:1609547/139‑1 (MQ=255)                         ccATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcggc                                                                                                           <  2:1445744/139‑1 (MQ=255)                          cATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcgg                                                                                                            >  2:984319/1‑137 (MQ=255)                          cATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcgg                                                                                                            <  1:984319/137‑1 (MQ=255)                          cATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCa                                                                                                          >  2:1456197/1‑139 (MQ=255)                           aTACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCaa                                                                                                         <  2:408038/139‑1 (MQ=255)                            tACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAAt                                                                                                        >  1:789926/1‑139 (MQ=255)                               cGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGa                                                                                                     <  2:1647281/139‑1 (MQ=255)                                 gCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTg                                                                                                                >  2:789339/1‑126 (MQ=255)                                 gCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTg                                                                                                                <  1:789339/126‑1 (MQ=255)                                   gAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                 <  2:1007239/139‑1 (MQ=255)                                    aGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACat                                                                                                >  1:474784/1‑139 (MQ=255)                                     ggAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                               <  2:648692/139‑1 (MQ=255)                                        aCTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTgg                                                                                                               >  1:1412704/1‑120 (MQ=255)                                        aCTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTgg                                                                                                               <  2:1412704/120‑1 (MQ=255)                                            ggCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGa                                                                                                     <  2:1031143/126‑1 (MQ=255)                                            ggCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGa                                                                                                     >  1:1031143/1‑126 (MQ=255)                                                tAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGt                                                                                                                              >  1:362212/1‑97 (MQ=255)                                                tAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGt                                                                                                                              <  2:362212/97‑1 (MQ=255)                                                 aaGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                   >  1:1230617/1‑139 (MQ=255)                                                  aGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgcg                                                                                  <  1:1870676/139‑1 (MQ=255)                                                       ttGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgcgtgcgt                                                                             >  1:1615009/1‑139 (MQ=255)                                                        tGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGtt                                                                            <  2:789926/139‑1 (MQ=255)                                                           ttCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAg                                                                         <  2:474784/139‑1 (MQ=255)                                                               cgcgCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCg                                                                                                                               <  1:1852348/81‑1 (MQ=255)                                                               cgcgCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCg                                                                                                                               >  2:1852348/1‑81 (MQ=255)                                                                     ttCGCATTACTGCCATCTCCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                 >  2:540069/1‑105 (MQ=255)                                                                     ttCGCATTACTGCCATCTCCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                 <  1:540069/105‑1 (MQ=255)                                                                      tCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATAcc                                                              >  2:1771390/1‑139 (MQ=255)                                                                       cGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATAccc                                                             >  1:1429814/1‑139 (MQ=255)                                                                              cTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcgg                                                      <  2:1615009/139‑1 (MQ=255)                                                                              cTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcgg                                                      <  1:1456197/139‑1 (MQ=255)                                                                              cTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcg                                                       >  1:1443149/1‑138 (MQ=255)                                                                              cTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcg                                                       <  2:1443149/138‑1 (MQ=255)                                                                                gCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCa                                                                                                          <  2:1376059/85‑1 (MQ=255)                                                                                gCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCa                                                                                                          >  1:1376059/1‑85 (MQ=255)                                                                                   aTCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTc                                                 <  2:1486333/139‑1 (MQ=255)                                                                                     cTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcg                                                                                                             <  2:1600218/77‑1 (MQ=255)                                                                                     cTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcg                                                                                                             >  1:1600218/1‑77 (MQ=255)                                                                                         aaCGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGAc                                           >  1:662193/1‑139 (MQ=255)                                                                                         aaCGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGAc                                           <  1:1653551/139‑1 (MQ=255)                                                                                                aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcggc                                                                                                           <  1:1724102/68‑1 (MQ=255)                                                                                                aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTggcggc                                                                                                           >  2:1724102/1‑68 (MQ=255)                                                                                                aaCGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCa                                    <  1:1771390/139‑1 (MQ=255)                                                                                                  cGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAg                                                                                                                                 >  2:703990/1‑44 (MQ=255)                                                                                                  cGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAg                                                                                                                                 <  1:703990/44‑1 (MQ=255)                                                                                                  cGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATa                                  <  2:1230617/139‑1 (MQ=255)                                                                                                    gCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATAcc                                <  2:1418665/139‑1 (MQ=255)                                                                                                       ggTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGcggcgg                                                   >  2:426439/1‑117 (MQ=255)                                                                                                       ggTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGcggcgg                                                   <  1:426439/117‑1 (MQ=255)                                                                                                       ggTTTTCGGCAATCATGGTCACGGCTTACAGTTCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCCTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAat                             <  2:245796/139‑1 (MQ=255)                                                                                                        gTTTTCGGCAATCATGGTCACGGCTTTCAGTGcgca                                                                                                                                   <  1:1778225/36‑1 (MQ=255)                                                                                                        gTTTTCGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAatc                            >  2:8988/1‑139 (MQ=255)                                                                                                             cGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcgg                                                      >  1:655207/1‑108 (MQ=255)                                                                                                             cGGCAATCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTgcgg                                                      <  2:655207/108‑1 (MQ=255)                                                                                                                  aTCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                   >  2:1345693/1‑74 (MQ=255)                                                                                                                  aTCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAgc                                                                                   <  1:1345693/74‑1 (MQ=255)                                                                                                                  aTCATGGTCACGGCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATACGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCAGCAGAgagg                  >  2:1114973/1‑139 (MQ=255)                                                                                                                             ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                 >  1:319519/1‑49 (MQ=255)                                                                                                                             ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACa                                                                                                 <  2:319519/49‑1 (MQ=255)                                                                                                                             ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCaa                              >  2:445237/1‑116 (MQ=255)                                                                                                                             ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCaa                              <  1:445237/116‑1 (MQ=255)                                                                                                                             ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCa                               <  1:1777346/115‑1 (MQ=255)                                                                                                                             ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCa                               >  2:1777346/1‑115 (MQ=255)                                                                                                                             ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACa       >  1:1403882/1‑139 (MQ=255)                                                                                                                             ggCTTTCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACa       >  1:604002/1‑139 (MQ=255)                                                                                                                                  tCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                               <  2:656111/46‑1 (MQ=255)                                                                                                                                  tCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACata                                                                                               >  1:656111/1‑46 (MQ=255)                                                                                                                                  tCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAatcat                          >  2:872106/1‑115 (MQ=255)                                                                                                                                  tCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAatcat                          <  1:872106/115‑1 (MQ=255)                                                                                                                                  tCAGTGCGCAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAag  <  2:1628760/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    AACCCAGAGAAGCATTATTGAACGCCATACCGGCGAGGAACTGGGCATAAGCCATTGCTTCACGCGCTTTCGCATTACTGCCATCTTCAACGGCTAACGGCAGGTTTTCGGCAATCATGGTCACGGCTTTCAGTGCACAAGCGTCAGTGATCGGCGTGGCGGCAATAGAAACATATGCTTCGATAGCGTGCGTTAAGGCATCCATACCCGTTGCGGCGGTCAGTGACTTCGGCATACCAATCATCAGAGAGGAGTCATTGACAGAAAG  >  NC_000913/3757047‑3757314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑