Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,761,850	C→T	A36T (GCG→ACG)	yibF ←	glutathione S‑transferase homolog

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,761,850	0	C	T	100.0%	217.6 / NA	67	A36T (GCG→ACG)	yibF	glutathione S‑transferase homolog
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/30);  total (37/30)

CGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGCGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAAT  >  NC_000913/3761722‑3761981                                                                                                                                 |                                                                                                                                    cGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                           <  1:794610/139‑1 (MQ=255)                 attAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                            <  1:1100417/122‑1 (MQ=255)                 attAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                            >  2:1100417/1‑122 (MQ=255)                                 cAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCtt                                                                                           >  1:14880/1‑139 (MQ=255)                                   gCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                            <  2:1563933/104‑1 (MQ=255)                                   gCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                            >  1:1563933/1‑104 (MQ=255)                                    cGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTc                                                                                        >  2:1601916/1‑139 (MQ=255)                                    cGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTc                                                                                        >  2:1091220/1‑139 (MQ=255)                                    cGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTc                                                                                        >  1:1854775/1‑139 (MQ=255)                                     gatgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTTTGCCCtctcct                                                                                       <  1:1091220/139‑6 (MQ=255)                                       tgatCGGCGAATCAGACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTACGCCACGCCGTTGTCCGTGTTATAggt                                                                                                                            >  2:1141170/1‑99 (MQ=255)                                       tgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                            <  1:1141170/100‑1 (MQ=255)                                       tgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGa                                                                                                        <  1:106464/120‑1 (MQ=255)                                       tgatCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGa                                                                                                        >  2:106464/1‑120 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTTGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCt                                                                                            <  1:1181554/130‑1 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                            >  1:705898/1‑98 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAggg                                                                                                                            <  2:705898/98‑1 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                           <  2:93625/99‑1 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                           >  1:93625/1‑99 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATtaat                                                                                                                >  2:1362382/1‑110 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATtaat                                                                                                                <  1:1362382/110‑1 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaaca                                                                                   >  2:784070/1‑139 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaaca                                                                                   >  1:777100/1‑139 (MQ=255)                                         atCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTGCATTAATAAATTCGAAAGTTATGCCCt                                                                                            >  2:1181554/1‑130 (MQ=255)                                          tCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaa                                                                                  <  1:917778/139‑1 (MQ=255)                                                   cAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                               >  2:722055/1‑117 (MQ=255)                                                   cAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                               <  1:722055/117‑1 (MQ=255)                                                   cAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGa                                                                         >  1:343625/1‑139 (MQ=255)                                                     aaCCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAg                                                                                                                         >  1:545769/1‑89 (MQ=255)                                                     aaCCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAg                                                                                                                         <  2:545769/89‑1 (MQ=255)                                                                gCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACa                                                            >  1:1449224/1‑139 (MQ=255)                                                                gCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACa                                                            >  1:208565/1‑139 (MQ=255)                                                                     ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                 <  2:228654/113‑1 (MQ=255)                                                                     ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                 >  1:228654/1‑113 (MQ=255)                                                                     ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaa                                                                                     <  2:1292815/109‑1 (MQ=255)                                                                     ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaa                                                                                     >  1:1292815/1‑109 (MQ=255)                                                                     ctTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACgg                                                       >  1:1260029/1‑139 (MQ=255)                                                                        cGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCt                                                    >  1:1854244/1‑139 (MQ=255)                                                                           tCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGa                                                                                                        <  1:491250/84‑1 (MQ=255)                                                                           tCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGa                                                                                                        >  2:491250/1‑84 (MQ=255)                                                                           tCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGgt                                                 >  1:628810/1‑139 (MQ=255)                                                                              ccAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                           <  2:582223/62‑1 (MQ=255)                                                                              ccAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGc                                                                                                                           >  1:582223/1‑62 (MQ=255)                                                                               cAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAg                                             <  2:628810/139‑1 (MQ=255)                                                                               cAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAg                                             >  2:834446/1‑139 (MQ=255)                                                                                aGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCt                                                    >  2:1495639/1‑131 (MQ=255)                                                                                aGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCt                                                    <  1:1495639/131‑1 (MQ=255)                                                                                                  aaCGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTa                                                                                                  >  2:1529012/1‑67 (MQ=255)                                                                                                  aaCGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTa                                                                                                  <  1:1529012/67‑1 (MQ=255)                                                                                                  aaCGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGATTTTCATGc                          >  1:1141915/1‑139 (MQ=255)                                                                                                  aaCGGGTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGc                          <  2:343625/139‑1 (MQ=255)                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacgac                                                                                 >  1:1348207/1‑79 (MQ=255)                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaac                                                                                 <  2:1348207/79‑1 (MQ=255)                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACggg                                                      <  2:1779060/106‑1 (MQ=255)                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACggg                                                      >  1:1779060/1‑106 (MQ=255)                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACgg                                                       >  1:104535/1‑105 (MQ=255)                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACgg                                                       <  2:104535/105‑1 (MQ=255)                                                                                                       gTTAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAATTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGt                     >  1:465120/1‑139 (MQ=255)                                                                                                         tAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaa                                                                                  >  2:1356202/1‑76 (MQ=255)                                                                                                         tAAATTGCGCCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTaacaa                                                                                  <  1:1356202/76‑1 (MQ=255)                                                                                                                 gcCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCtttt                                                                                         <  2:96845/61‑1 (MQ=255)                                                                                                                 gcCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCtttt                                                                                         >  1:96845/1‑61 (MQ=255)                                                                                                                 gcCACGCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCg           <  2:1260029/139‑1 (MQ=255)                                                                                                                      gCCGTTGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGaa      <  1:1881043/139‑1 (MQ=255)                                                                                                                          ttGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                               <  1:1319240/46‑1 (MQ=255)                                                                                                                          ttGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGc                                                                                               >  2:1319240/1‑46 (MQ=255)                                                                                                                          ttGTCCGTGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAat  <  2:1854244/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    CGCCGGAGCGACATTCATTAATTCAATATATTCAGCGATGATCGGCGAATCAAACCAGCATTCGCCCTCTTCGGTCACCAGCACCGGCACTTTTCCTAACGGGTTAAATTGCGCCACGCCGTTGTCCGCGTTATAGGGCAGTTCATTAATAAATTCGAAAGTTATGCCCTTTTCTAACAACAGAATAGAAAGTTTGCGTACAAACGGGCTGGTGTAGCTACCGACGAGTTTCATGCCGAGTCCTTTGTGCGAGGAAAAAT  >  NC_000913/3761722‑3761981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑