Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,898,609	G→A	*196* (TAG→TAA)	cbrC →	UPF0167 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,898,609	0	G	A	100.0%	196.7 / NA	60	*196* (TAG→TAA)	cbrC	UPF0167 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/30);  total (30/30)

AAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACA  >  NC_000913/3898479‑3898741                                                                                                                                   |                                                                                                                                     aagaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTGATTATCAGGGTTATCTCTTCCGCTCTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              <  2:1150283/139‑1 (MQ=255) aagaTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              <  1:623445/139‑1 (MQ=255)    aTTGTGAGAATTTCGGTGTTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaata                                                                                                                           >  2:1177120/1‑139 (MQ=255)    aTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaata                                                                                                                           >  1:443513/1‑139 (MQ=255)    aTTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTA‑‑TCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaataat                                                                                                                         >  2:364598/1‑139 (MQ=255)                    tATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                                           <  2:443513/139‑1 (MQ=255)                    tATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                                           <  2:1074504/139‑1 (MQ=255)                              tCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                                 <  1:158082/139‑1 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              <  2:1745858/91‑1 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              >  1:1745858/1‑91 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaataat                                                                                                                         >  2:1774840/1‑96 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAaataat                                                                                                                         <  1:1774840/96‑1 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGc                                                                                                               <  1:1095306/106‑1 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGc                                                                                                               >  2:1095306/1‑106 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                              >  2:1193723/1‑139 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                              >  1:601676/1‑139 (MQ=255)                                                 tGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCt                                                                              <  2:1091832/139‑1 (MQ=255)                                                   cAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGaa                                                                                                                    <  2:1463928/99‑1 (MQ=255)                                                   cAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGaa                                                                                                                    >  1:1463928/1‑99 (MQ=255)                                                        gggTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTa                                                                                                                                   >  1:330313/1‑79 (MQ=255)                                                        gggTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTa                                                                                                                                   <  2:330313/79‑1 (MQ=255)                                                             gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCg                                                                  <  1:1177120/139‑1 (MQ=255)                                                             gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCATAATTCACACATc                                                                                           >  2:1057169/1‑114 (MQ=255)                                                             gtCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCATAATTCACACATc                                                                                           <  1:1057169/114‑1 (MQ=255)                                                                       gggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAAtt                                                        >  2:681479/1‑139 (MQ=255)                                                                        ggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              <  1:144027/68‑1 (MQ=255)                                                                        ggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              >  2:144027/1‑68 (MQ=255)                                                                        ggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                                 <  1:1399796/97‑1 (MQ=255)                                                                        ggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTca                                                                                                 >  2:1399796/1‑97 (MQ=255)                                                                        ggTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTc                                                       >  2:1494750/1‑139 (MQ=255)                                                                            aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                             <  2:197034/81‑1 (MQ=255)                                                                            aTCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCg                                                                                                             >  1:197034/1‑81 (MQ=255)                                                                               tctTCCGCTGTCTCCACTGCGGCAAGCTGGGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCa                                                <  1:1230715/139‑1 (MQ=255)                                                                                  tCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCg                                             >  2:1881722/1‑139 (MQ=255)                                                                                   ccGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              <  2:41799/57‑1 (MQ=255)                                                                                   ccGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              >  1:41799/1‑57 (MQ=255)                                                                                      cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCtt                                                                                    <  1:607362/96‑1 (MQ=255)                                                                                      cTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCtt                                                                                    >  2:607362/1‑96 (MQ=255)                                                                                         tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                                           >  1:1007663/1‑70 (MQ=255)                                                                                         tctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                                           <  2:1007663/70‑1 (MQ=255)                                                                                          ctcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATc                                     <  1:364598/139‑1 (MQ=255)                                                                                           tcCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCa                                    >  2:872298/1‑139 (MQ=255)                                                                                             cACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTa                                                                      >  2:1335983/1‑103 (MQ=255)                                                                                             cACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTa                                                                      <  1:1335983/103‑1 (MQ=255)                                                                                               cTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGa                                <  1:1881722/139‑1 (MQ=255)                                                                                                   ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              >  2:877776/1‑41 (MQ=255)                                                                                                   ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              <  1:877776/41‑1 (MQ=255)                                                                                                   ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATcc                                                                                          >  2:1207009/1‑77 (MQ=255)                                                                                                   ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATcc                                                                                          <  1:1207009/77‑1 (MQ=255)                                                                                                   ggCAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTAc                            >  2:2222/1‑139 (MQ=255)                                                                                                     cAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              <  2:751146/39‑1 (MQ=255)                                                                                                     cAAGCTGAGACTGTGGGGTGATTTTTCGTAATTATTTaa                                                                                                                              >  1:751146/1‑39 (MQ=255)                                                                                                                  tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                                           >  2:887543/1‑45 (MQ=255)                                                                                                                  tgGGGTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGa                                                                                                           <  1:887543/45‑1 (MQ=255)                                                                                                                   ggggTGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGc            >  1:1228245/1‑139 (MQ=255)                                                                                                                       tGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGa                                <  2:597803/115‑1 (MQ=255)                                                                                                                       tGATTTTTCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGa                                >  1:597803/1‑115 (MQ=255)                                                                                                                          tttttCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTa                                                                                   <  2:1629017/61‑1 (MQ=255)                                                                                                                          tttttCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTa                                                                                   >  1:1629017/1‑61 (MQ=255)                                                                                                                             ttCGTAATTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAaca  <  1:2222/139‑1 (MQ=255)                                                                                                                                   |                                                                                                                                     AAGATTGTGAGAATTTCGGTATTAGAAATTCTGATCTAGCTAAATGCCTGCAAAAGGGTGGTCATTGTCAGGGTTATCTCTTCCGCTGTCTCCACTGCGGCAAGCTGAGACTGTGGGGTGATTTTTCGTAGTTATTTAAATAATGAGAACAGGCCGGAGCGTAATTCACACATCCGGCCTTATTTCTTAAGCTTAACCGAGTGCTAATTCTGCGGCAGCGGCTTTATCAGCGATTACCATCAGCGAAGGATGCAGCTGCAACA  >  NC_000913/3898479‑3898741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑