Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,077	G→A	*349* (TAG→TAA)	wzzE →	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,077	0	G	A	100.0%	184.6 / NA	56	*349* (TAG→TAA)	wzzE	Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

TATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑G‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAA  >  NC_000913/3969943‑3970206                                                                                                                                         |                                                                                                                                  tATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                                <  2:123480/139‑1 (MQ=255)  aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                                                                               >  1:132874/1‑139 (MQ=255)  aTCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATaacaac                                                                                                                               >  1:967866/1‑139 (MQ=255)          ttGCGTACGCCGGAAGAACCGGCAAAACGCAATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTC‑GCGGGGGCTGATCGGAGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACtactgc                                                                                                                       >  1:621380/1‑139 (MQ=255)                 cGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAgc                                                                                                                >  2:652511/1‑139 (MQ=255)                            ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATT‑ACCCGCCGTTGCTCGAAATAACaa                                                                                                                                <  1:1563589/111‑1 (MQ=255)                            ccGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATT‑ACCCGCCGTTGCTCGAAATAACaa                                                                                                                                >  2:1563589/1‑111 (MQ=255)                                 aaaaCGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                                <  2:132874/139‑1 (MQ=255)                                                aCGTCGTGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa                                                                                 >  2:1747900/1‑139 (MQ=255)                                                 cgtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAAt                                                                                <  1:1747900/139‑1 (MQ=255)                                                  gtcgtGCCTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATc                                                                               >  2:1521452/1‑139 (MQ=255)                                                         cTTCCTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGa                                                                        <  2:1839495/139‑1 (MQ=255)                                                            ccTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                           >  2:572536/1‑133 (MQ=255)                                                            ccTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGAtg                                                                           <  1:572536/133‑1 (MQ=255)                                                            ccTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAg                                                                     >  2:832671/1‑139 (MQ=255)                                                             cTGATGATTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGt                                                                    <  2:525286/139‑1 (MQ=255)                                                              tgatgaTTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                   <  1:652511/139‑1 (MQ=255)                                                                  gatTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                               <  1:841782/139‑1 (MQ=255)                                                                  gatTATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                               <  1:832671/139‑1 (MQ=255)                                                                     tATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                                                                              <  2:760557/73‑1 (MQ=255)                                                                     tATGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAacaaca                                                                                                                              >  1:760557/1‑73 (MQ=255)                                                                      aTGCGGGGCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGt                                                           <  1:1792589/139‑1 (MQ=255)                                                                       tgcggggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                   >  1:805275/4‑130 (MQ=255)                                                                       tgcggggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTa                                                                   <  2:805275/127‑1 (MQ=255)                                                                       tgcggggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTa                                                          >  1:405511/4‑139 (MQ=255)                                                                           gggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                                >  1:761221/1‑65 (MQ=255)                                                                           gggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACaa                                                                                                                                <  2:761221/65‑1 (MQ=255)                                                                            ggCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTgg                                                     >  2:1785386/1‑139 (MQ=255)                                                                             gCATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGt                                                    <  1:1521452/139‑1 (MQ=255)                                                                              cATTGTCGG‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTa                                                   <  2:967866/139‑1 (MQ=255)                                                                                 tgtcgg‑gggggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTAcgc                                                <  1:982578/134‑1 (MQ=255)                                                                                           ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                                >  2:605942/1‑82 (MQ=255)                                                                                           ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTcgccg                                                                                                <  1:605942/82‑1 (MQ=255)                                                                                           ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAg                                       >  2:658235/1‑139 (MQ=255)                                                                                           ggCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAg                                       >  1:245527/1‑139 (MQ=255)                                                                                             cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                              <  2:1356642/82‑1 (MQ=255)                                                                                             cTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                              >  1:1356642/1‑82 (MQ=255)                                                                                              tGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCa                                    >  1:1061558/1‑139 (MQ=255)                                                                                              tGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCa                                    >  2:485250/1‑139 (MQ=255)                                                                                                    gggCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGa                              >  2:1553326/1‑139 (MQ=255)                                                                                                       cTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATgg                           <  2:405511/139‑1 (MQ=255)                                                                                                          gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa                                                                                 >  2:1504387/1‑82 (MQ=255)                                                                                                          gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGaa                                                                                 <  1:1504387/82‑1 (MQ=255)                                                                                                          gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                               >  2:639397/1‑100 (MQ=255)                                                                                                          gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTactga                                                               <  1:639397/100‑1 (MQ=255)                                                                                                          gtgtCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGgcgc                        >  2:1033743/1‑139 (MQ=255)                                                                                                             tCGCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGt                     <  2:656260/139‑1 (MQ=255)                                                                                                               gCATTAACCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTg                   >  1:734034/1‑139 (MQ=255)                                                                                                                    aaCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGa                                                                                                                  <  2:1228160/39‑1 (MQ=255)                                                                                                                    aaCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGa                                                                                                                  >  1:1228160/1‑39 (MQ=255)                                                                                                                     aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                              <  2:55063/58‑1 (MQ=255)                                                                                                                     aCCCGCCGTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCt                                                                                              >  1:55063/1‑58 (MQ=255)                                                                                                                         gccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                      >  2:27954/1‑62 (MQ=255)                                                                                                                         gccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                      <  1:909642/62‑1 (MQ=255)                                                                                                                         gccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                      <  1:27954/62‑1 (MQ=255)                                                                                                                         gccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGaa                                                                                      >  2:909642/1‑62 (MQ=255)                                                                                                                          ccgTTGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGtt        <  1:1785386/139‑1 (MQ=255)                                                                                                                              tGCTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCa    >  1:75584/1‑139 (MQ=255)                                                                                                                                cTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCaaa  >  2:303520/1‑139 (MQ=255)                                                                                                                                cTCGAAATAACAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCaaa  >  1:924457/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                  TATCGCTATTTGCGTACGCCGGAAGAACCGGTAAAACGCGATAGCCCACGTCGTGCCTTCCTGATGATTATGTGGGGCATTGTC‑G‑GGGGGCTGATCGGGGCTGGTGTCGCATTAACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAA  >  NC_000913/3969943‑3970206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑