Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,976,605	T→C	F110L (TTT→CTT)	wzxE →	O‑antigen translocase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,976,605	0	T	C	100.0%	237.3 / NA	67	F110L (TTT→CTT)	wzxE	O‑antigen translocase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/35);  total (32/35)

TGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTT  >  NC_000913/3976475‑3976726                                                                                                                                     |                                                                                                                          tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAAt                                                                                                                   >  2:1043157/1‑139 (MQ=255)    ccAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATAcc                                                                                                                <  1:522664/139‑1 (MQ=255)      aGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGa                                                                                                              <  2:785209/139‑1 (MQ=255)       gTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGAc                                                                                                             <  1:1225287/139‑1 (MQ=255)           cATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATc                                                                                                         >  1:113037/1‑139 (MQ=255)             tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCGTCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAg                                                                                                       >  1:1451971/1‑139 (MQ=255)             tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAg                                                                                                       >  2:1357438/1‑139 (MQ=255)             tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAg                                                                                                       >  1:330739/1‑139 (MQ=255)                  aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAgggg                                                                                                    >  1:335816/1‑139 (MQ=255)                   tCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTg                                                                                                 <  1:139307/139‑1 (MQ=255)                     cGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGa                                                                                                              >  2:1227467/1‑124 (MQ=255)                     cGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGa                                                                                                              <  1:1227467/124‑1 (MQ=255)                         aCAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTg                                                                                                 <  1:1400580/133‑1 (MQ=255)                         aCAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTg                                                                                                 >  2:1400580/1‑133 (MQ=255)                               gcgcCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTg                                                                                     >  2:30274/1‑139 (MQ=255)                                     cgTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTg                                                                               >  2:443397/1‑139 (MQ=255)                                        ggTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTACGTTTAGTGGCGCTGGTg                                                                            <  1:1043157/139‑1 (MQ=255)                                            ggCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCaaa                                                                        <  2:113037/139‑1 (MQ=255)                                                 ttcgtcaGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                   <  2:1451971/135‑1 (MQ=255)                                                   catcaGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATa                                                                                                                  >  1:262238/1‑90 (MQ=255)                                                   catcaGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATa                                                                                                                  <  2:262238/90‑1 (MQ=255)                                                      caGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGc                                                              <  2:330739/139‑1 (MQ=255)                                                        gCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCt                                                                                                  <  1:244758/101‑1 (MQ=255)                                                        gCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCt                                                                                                  >  2:244758/1‑101 (MQ=255)                                                        gCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTg                                                                                                 <  2:371990/102‑1 (MQ=255)                                                        gCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTg                                                                                                 >  1:371990/1‑102 (MQ=255)                                                           aTGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGt                                                                             >  1:611420/1‑119 (MQ=255)                                                           aTGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGt                                                                             <  2:611420/119‑1 (MQ=255)                                                           aTGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTggg                                                         >  1:330274/1‑139 (MQ=255)                                                            tGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                        <  1:288072/139‑1 (MQ=255)                                                            tGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                        <  1:30274/139‑1 (MQ=255)                                                             tgTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGc                                                       <  1:216318/138‑1 (MQ=255)                                                             ggTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGc                                                       >  2:1086001/1‑139 (MQ=255)                                                             ggTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGc                                                       >  2:637262/1‑139 (MQ=255)                                                                          tctcTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            <  1:58561/107‑1 (MQ=255)                                                                          tctcTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            >  2:58561/1‑107 (MQ=255)                                                                           ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTg                                           >  1:961930/1‑139 (MQ=255)                                                                            tctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTgg                                          >  2:430507/1‑139 (MQ=255)                                                                               aCGCTGATGGCGCTGGTTTTTGTGCTGGCGGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGgcgc                                       <  2:1088309/139‑1 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGGTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtgatga                                 >  2:1239885/1‑139 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGTTGCGTTTAGTGGCGCTGATGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtgatga                                 >  1:1208006/1‑139 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcg                                                                                  >  1:416719/1‑90 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcg                                                                                  <  2:416719/90‑1 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            <  1:412669/96‑1 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            >  2:412669/1‑96 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                   >  1:893316/1‑105 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                   <  2:893316/105‑1 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtgatga                                 <  2:682084/139‑1 (MQ=255)                                                                                     aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtgatga                                 >  2:1846268/1‑139 (MQ=255)                                                                                          gcTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            <  2:1294571/91‑1 (MQ=255)                                                                                          gcTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                            >  1:1294571/1‑91 (MQ=255)                                                                                               tttttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcg                       >  2:980878/1‑139 (MQ=255)                                                                                                 tttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcg                     <  1:637262/139‑1 (MQ=255)                                                                                                 tttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcg                     <  1:443397/139‑1 (MQ=255)                                                                                                 tttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcg                     <  1:1086001/139‑1 (MQ=255)                                                                                                       cTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg               <  2:330274/139‑1 (MQ=255)                                                                                                        tGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGc              <  2:335816/139‑1 (MQ=255)                                                                                                         ggCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGAc                                                                                                             >  2:514376/1‑43 (MQ=255)                                                                                                         ggCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGAc                                                                                                             <  1:514376/43‑1 (MQ=255)                                                                                                                  gcCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCg    <  2:961930/139‑1 (MQ=255)                                                                                                                    cAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGtt  <  1:980878/139‑1 (MQ=255)                                                                                                                           ccAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGcc                                                             >  1:72192/1‑73 (MQ=255)                                                                                                                           ccAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGcc                                                             <  2:72192/73‑1 (MQ=255)                                                                                                                                gATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCaaa                                                                        >  2:1290094/1‑57 (MQ=255)                                                                                                                                gATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCaaa                                                                        <  1:1290094/57‑1 (MQ=255)                                                                                                                                gATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcg                     <  2:1713276/108‑1 (MQ=255)                                                                                                                                gATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcg                     >  1:1713276/1‑108 (MQ=255)                                                                                                                                     |                                                                                                                          TGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTT‑‑TCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTT  >  NC_000913/3976475‑3976726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑