Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,061,157	G→A	*237* (TAG→TAA)	yihL →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,061,157	0	G	A	100.0%	277.8 / NA	84	*237* (TAG→TAA)	yihL	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/43);  total (41/43)

TTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACT  >  NC_000913/4061030‑4061286                                                                                                                                |                                                                                                                                  ttGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        <  2:333699/139‑1 (MQ=255)   ggCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTg                                                                                                                      <  2:151325/139‑1 (MQ=255)   ggCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTg                                                                                                                      >  1:151325/1‑139 (MQ=255)           tGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACaataaa                                                                                                              >  2:1231166/1‑139 (MQ=255)             cGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACaataaata                                                                                                            >  2:1484428/1‑139 (MQ=255)               ggTATGCCAGCGATCTTAACTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAAc                                                                                                          <  1:1231166/139‑1 (MQ=255)                    gCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTACGGTGACAATAAATAACGCaag                                                                                                     >  2:1104691/1‑139 (MQ=255)                         cGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaaga                                                                                                >  1:233208/1‑139 (MQ=255)                          gATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGAt                                                                                               >  2:1770131/1‑139 (MQ=255)                           aTCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGAtt                                                                                              <  1:1184802/139‑1 (MQ=255)                             cTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        >  2:718560/1‑111 (MQ=255)                             cTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        <  1:718560/111‑1 (MQ=255)                              tgaaTTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATATATAACGCAAGAAAGATTCTa                                                                                           <  1:1104691/137‑1 (MQ=255)                               tAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc                                                                                          <  2:233208/139‑1 (MQ=255)                                aaTTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        >  1:1130898/1‑108 (MQ=255)                                aaTTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        <  2:1130898/108‑1 (MQ=255)                                     aaaaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                                   >  2:857882/1‑108 (MQ=255)                                     aaaaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                                   <  1:857882/108‑1 (MQ=255)                                      aaaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACg                                                                                      <  2:1585924/136‑1 (MQ=255)                                      aaaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGACGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACg                                                                                      >  1:1585924/1‑136 (MQ=255)                                       aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTTGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        <  2:1454983/101‑1 (MQ=255)                                       aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        >  1:1454983/1‑101 (MQ=255)                                       aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                                   <  1:166174/106‑1 (MQ=255)                                       aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACa                                                                                                                   >  2:166174/1‑106 (MQ=255)                                       aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                   >  1:1313135/1‑122 (MQ=255)                                       aaGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                   <  2:1313135/122‑1 (MQ=255)                                        aGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCg                                                                                 >  2:155198/1‑139 (MQ=255)                                         gCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGa                                                                                <  2:1347825/139‑1 (MQ=255)                                          cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc                                                                                          >  2:1337080/1‑128 (MQ=255)                                          cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc                                                                                          <  1:1337080/128‑1 (MQ=255)                                          cATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGac                                                                               >  1:1265312/1‑139 (MQ=255)                                             cgcgCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACAct                                                                            <  1:1484428/139‑1 (MQ=255)                                                    aCCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACActc                                                                           <  1:1190903/133‑1 (MQ=255)                                                    aCCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGCGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACActc                                                                           >  2:1190903/1‑133 (MQ=255)                                                             gCAATATTGTCCAAATAGCTATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTAc                                                            <  2:1265312/139‑1 (MQ=255)                                                                      tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                                                  <  2:1468381/92‑1 (MQ=255)                                                                      tCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaaa                                                                                                  >  1:1468381/1‑92 (MQ=255)                                                                       ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc                                                                                          >  1:1271248/1‑99 (MQ=255)                                                                       ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc                                                                                          <  2:1271248/99‑1 (MQ=255)                                                                       ccAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGc                                                  >  1:487342/1‑139 (MQ=255)                                                                        cAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGc                                                                                                        <  1:850745/84‑1 (MQ=255)                                                                        cAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGc                                                                                                        >  2:850745/1‑84 (MQ=255)                                                                           aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGa                                                                                                                     >  1:481712/1‑68 (MQ=255)                                                                           aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGa                                                                                                                     <  2:481712/68‑1 (MQ=255)                                                                           aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGc                                                                                                        >  2:1513970/1‑81 (MQ=255)                                                                           aTAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGc                                                                                                        <  1:1513970/81‑1 (MQ=255)                                                                               atatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaag                                                                                                     <  2:951118/80‑1 (MQ=255)                                                                               atatTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaag                                                                                                     >  1:951118/1‑80 (MQ=255)                                                                                 atTGAATATTGGCGATTTGAGGCCGTAGTCCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaag                                                                                                     >  2:1162648/1‑78 (MQ=255)                                                                                 atTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaag                                                                                                     <  1:1162648/78‑1 (MQ=255)                                                                                     aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                   >  1:1862050/1‑76 (MQ=255)                                                                                     aaTATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaagaa                                                                                                   <  2:1862050/76‑1 (MQ=255)                                                                                       tatTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATg                                  <  2:487342/139‑1 (MQ=255)                                                                                          tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        >  1:227137/1‑50 (MQ=255)                                                                                          tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATgg                                                                                                                        <  2:227137/50‑1 (MQ=255)                                                                                          tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca                                                                              <  2:989562/92‑1 (MQ=255)                                                                                          tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGaca                                                                              >  1:989562/1‑92 (MQ=255)                                                                                          tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGa                                                                                <  2:865921/90‑1 (MQ=255)                                                                                          tGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGa                                                                                >  1:865921/1‑90 (MQ=255)                                                                                           ggCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGc                              <  1:1770131/139‑1 (MQ=255)                                                                                           ggCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGc                              <  2:600367/139‑1 (MQ=255)                                                                                            gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATACATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtt                                                                   <  1:1328510/101‑1 (MQ=255)                                                                                            gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtt                                                                   >  2:1328510/1‑101 (MQ=255)                                                                                            gCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt                             >  1:1806211/1‑139 (MQ=255)                                                                                               aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaag                                                                                                     >  2:1500325/1‑64 (MQ=255)                                                                                               aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCaag                                                                                                     <  1:1500325/64‑1 (MQ=255)                                                                                               aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt                             >  1:871795/1‑136 (MQ=255)                                                                                               aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCt                             <  2:871795/136‑1 (MQ=255)                                                                                               aTTTGAGGCCGTAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGa                          >  2:469206/1‑139 (MQ=255)                                                                                                          tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtt                                                                   <  2:168889/87‑1 (MQ=255)                                                                                                          tAGACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCtt                                                                   >  1:168889/1‑87 (MQ=255)                                                                                                            gACCTCATCATTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCct             <  2:1806211/139‑1 (MQ=255)                                                                                                                  atcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt       <  1:469206/139‑1 (MQ=255)                                                                                                                   tcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt       >  2:291443/1‑138 (MQ=255)                                                                                                                   tcatTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGAt       <  1:291443/138‑1 (MQ=255)                                                                                                                    catTAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAATATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                                <  1:434961/96‑1 (MQ=255)                                                                                                                    catTAATCTGTAAGTGATTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCt                                                >  2:434961/1‑96 (MQ=255)                                                                                                                       tAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCtt                                                                         <  1:951413/68‑1 (MQ=255)                                                                                                                       tAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCtt                                                                         >  2:951413/1‑68 (MQ=255)                                                                                                                       tAATCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACt  >  1:1363684/1‑139 (MQ=255)                                                                                                                         aTCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc                                                                                          >  2:134433/1‑49 (MQ=255)                                                                                                                         aTCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTAc                                                                                          <  1:134433/49‑1 (MQ=255)                                                                                                                         aTCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTa                                                     <  2:1734823/86‑1 (MQ=255)                                                                                                                         aTCTGTAAGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTa                                                     >  1:1734823/1‑86 (MQ=255)                                                                                                                                |                                                                                                                                  TTGGCGGTTCTGCGGGTATGCCAGCGATCTTAATTGAAAAGCATCGCGCCGACCAGCAAGGCAATATTGTCCAAATAGATATTGAATATTGGCGATTTGAGGCCGTAGACCTCATCATTAATCTGTAGGTGTTTTATGGTGACAATAAATAACGCAAGAAAGATTCTACAACGTGTCGACACTCTTCCTCTTTATTTACATGCTTATGCCTTTCATTTAAATATGCGGCTGGAAAGAGTGTTGCCTGCTGATTTACT  >  NC_000913/4061030‑4061286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑