Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,078,438	G→A	*330* (TAG→TAA)	yiiD →	GNAT family putative N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,078,438	0	G	A	100.0%	190.0 / NA	59	*330* (TAG→TAA)	yiiD	GNAT family putative N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/33);  total (26/33)

GGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTA  >  NC_000913/4078315‑4078565                                                                                                                            |                                                                                                                                ggTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCATTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGc                                                                                                                  >  1:438946/1‑139 (MQ=255)         tGCAGGTCGAAATCTTTGGCGATGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          <  1:1879763/139‑1 (MQ=255)         tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          <  1:1242473/139‑1 (MQ=255)         tGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          <  1:1461701/139‑1 (MQ=255)            aGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          >  2:975583/1‑136 (MQ=255)            aGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          <  1:975583/136‑1 (MQ=255)                       tttGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAgg                                                                                            <  2:463530/139‑1 (MQ=255)                        ttGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGt                                                                                           >  1:1284285/1‑139 (MQ=255)                           gCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCATTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCAt                                                                                        <  2:438946/139‑1 (MQ=255)                              acgaGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTc                                                                                          <  2:1459393/134‑1 (MQ=255)                              acgaGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTc                                                                                          >  1:1459393/1‑134 (MQ=255)                                 agaCGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTatat                                                                                  <  2:651864/139‑1 (MQ=255)                                 agaCGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTatat                                                                                  <  2:552956/139‑1 (MQ=255)                                                       aGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTta                                                            >  1:1353360/1‑139 (MQ=255)                                                          cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAttt                                                                  >  1:902400/1‑130 (MQ=255)                                                          cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAttt                                                                  <  2:902400/130‑1 (MQ=255)                                                          cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat                                                         <  1:847364/139‑1 (MQ=255)                                                          cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat                                                         <  2:1284285/139‑1 (MQ=255)                                                          cACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat                                                         <  2:1007122/139‑1 (MQ=255)                                                            cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          >  1:177984/1‑88 (MQ=255)                                                            cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          <  2:177984/88‑1 (MQ=255)                                                            cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          >  1:868464/1‑88 (MQ=255)                                                            cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          <  2:868464/88‑1 (MQ=255)                                                            cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtg                                                                                                              <  1:1236378/84‑1 (MQ=255)                                                            cGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtg                                                                                                              >  2:1236378/1‑84 (MQ=255)                                                                       cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGGTGGAGAGGGGCGGTGCTGCCTCTCTCATTCAg                                                                                             <  1:1114852/90‑1 (MQ=255)                                                                       cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          >  1:88674/1‑77 (MQ=255)                                                                       cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          <  2:88674/77‑1 (MQ=255)                                                                       cTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAAAGGGGCGGTGCTGCCTCTCTCATTCAg                                                                                             >  2:1114852/1‑90 (MQ=255)                                                                        tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGa                                                                                                                             >  2:644419/1‑57 (MQ=255)                                                                        tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGa                                                                                                                             <  1:644419/57‑1 (MQ=255)                                                                        tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat                                                         >  2:1682968/1‑125 (MQ=255)                                                                        tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTatat                                                         <  1:1682968/125‑1 (MQ=255)                                                                        tGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAActct                                           >  2:84006/1‑139 (MQ=255)                                                                          cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCct                                                                                                        <  1:657578/76‑1 (MQ=255)                                                                          cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCct                                                                                                        >  2:657578/1‑76 (MQ=255)                                                                          cccGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCtat                                         >  1:1863095/1‑139 (MQ=255)                                                                              cgAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtg                                                                                                              >  2:1075385/1‑66 (MQ=255)                                                                              cgAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACCGATGGAGAGGGGCGGtg                                                                                                              <  1:1075385/66‑1 (MQ=255)                                                                                      tttGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAttt                                                                  >  1:448923/1‑102 (MQ=255)                                                                                      tttGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTAttt                                                                  <  2:448923/102‑1 (MQ=255)                                                                                            ccGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTgg                                                                        <  1:83482/90‑1 (MQ=255)                                                                                            ccGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTgg                                                                        >  2:83482/1‑90 (MQ=255)                                                                                            ccGTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGtt                       <  1:84006/139‑1 (MQ=255)                                                                                              gTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGgag                                                                                                                        >  1:1279561/1‑40 (MQ=255)                                                                                              gTATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATCgag                                                                                                                        <  2:1279561/40‑1 (MQ=255)                                                                                               tATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAgggg                                                                                                                   >  2:1237182/1‑44 (MQ=255)                                                                                               tATGAAGAGGGCGGGAACGAAGAAGAGTAACTGATGGAGAgggg                                                                                                                   <  1:1237182/44‑1 (MQ=255)                                                                                                         gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCgg                                                                                                                <  2:1270251/37‑1 (MQ=255)                                                                                                         gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCgg                                                                                                                >  1:1270251/1‑37 (MQ=255)                                                                                                         gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCg                         >  1:1595391/1‑124 (MQ=255)                                                                                                         gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCg                         <  2:1595391/124‑1 (MQ=255)                                                                                                         gCGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtg          >  1:563298/1‑139 (MQ=255)                                                                                                          cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTcc                                                                            <  1:1440133/72‑1 (MQ=255)                                                                                                          cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTcc                                                                            >  2:1440133/1‑72 (MQ=255)                                                                                                          cGGGAACGAAGAAGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACgtgt         <  1:1383055/139‑1 (MQ=255)                                                                                                             gAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          >  2:1720370/1‑39 (MQ=255)                                                                                                             gAACGAAGAAGAGTAACTGATGGAGAGGGGCGGtgctgc                                                                                                          <  1:1720370/39‑1 (MQ=255)                                                                                                                 gaagaaGAGTAACTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTa  <  2:1863095/139‑1 (MQ=255)                                                                                                                            |                                                                                                                                GGTGCAGATGCAGGTCGAAATCTTTGGCGACGAGACGCCGGGTGCAGTGTTTGAAGGCACGTATATCGTTCTGCCCGCGAAGCCATTTGGCCCGTATGAAGAGGGCGGGAACGAAGAAGAGTAGCTGATGGAGAGGGGCGGTGCTGCCTCTCTCATTCAGGTCATTTATATTAGTCCTTGGCTATTTCGGTTATATTAAATAAGAACTCTATAAATTTTTATTCTTCGTTGGCTCCATACGTGTCTTGCTA  >  NC_000913/4078315‑4078565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑