Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,188,124 A→G E925E (GAA→GAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,188,1240AG100.0% 305.7 / NA 85E925E (GAA→GAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/40);  total (45/40)

GTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAG  >  NC_000913/4187993‑4188258
                                                                                                                                   |                                                                                                                                      
gTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTa                                                                                                                                 <  1:1449738/139‑1 (MQ=255)
   cGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTacac                                                                                                                              <  1:735978/139‑1 (MQ=255)
   cGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTacac                                                                                                                              >  1:1759773/1‑139 (MQ=255)
     ttCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAg                                                                                                                            >  2:1413004/1‑139 (MQ=255)
        tgttgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTg                                                                                                                         >  2:1552898/1‑139 (MQ=255)
           tgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTac                                                                                                                                >  1:667586/1‑129 (MQ=255)
           tgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTac                                                                                                                                <  2:667586/129‑1 (MQ=255)
           tgtATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGAcc                                                                                                                      >  2:1620355/1‑139 (MQ=255)
             tATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCAt                                                                                                                    >  2:621452/1‑139 (MQ=255)
                 ttGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGt                                                                                                                <  1:163278/139‑1 (MQ=255)
                  tgtgACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTa                                                                                                               >  2:161732/1‑139 (MQ=255)
                       cacCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTc                                                                                                          <  1:161732/139‑1 (MQ=255)
                        acCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGACCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTcc                                                                                                         >  2:1873163/1‑139 (MQ=255)
                            aCTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACAt                                                                                                     >  2:961968/1‑139 (MQ=255)
                            aCTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACAt                                                                                                     >  2:1639826/1‑139 (MQ=255)
                                        gtgCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGc                                                                                         <  2:689008/139‑1 (MQ=255)
                                           cgcACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCg                                                                                                                 <  2:1267523/112‑1 (MQ=255)
                                           cgcACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCg                                                                                                                 >  1:1267523/1‑112 (MQ=255)
                                              aCTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCg                                                                                   <  1:1552898/139‑1 (MQ=255)
                                              aCTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCg                                                                                   <  2:1876142/139‑1 (MQ=255)
                                                 gCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGcc                                                                                                                                      >  2:1703501/1‑85 (MQ=255)
                                                  cTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGcc                                                                                                                                      <  2:1009209/84‑1 (MQ=255)
                                                     cGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCg                                                                                           >  2:1463651/1‑124 (MQ=255)
                                                     cGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCg                                                                                           <  1:1463651/124‑1 (MQ=255)
                                                      ggTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGgctg                                                                           <  2:1759773/139‑1 (MQ=255)
                                                        tCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGgctgct                                                                         >  1:350908/1‑139 (MQ=255)
                                                          gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCcac                                                                                                       >  1:1592396/1‑107 (MQ=255)
                                                          gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCcac                                                                                                       <  2:1592396/107‑1 (MQ=255)
                                                          gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGa                                                                       >  2:1497025/1‑139 (MQ=255)
                                                          gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGa                                                                       >  1:201412/1‑139 (MQ=255)
                                                          gTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGa                                                                       >  2:208883/1‑139 (MQ=255)
                                                            gACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTa                                                                                                                                 <  2:611132/79‑1 (MQ=255)
                                                            gACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTa                                                                                                                                 >  1:611132/1‑79 (MQ=255)
                                                            gACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGgctgct                                                                         >  1:1872920/1‑135 (MQ=255)
                                                            gACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGgctgct                                                                         <  2:1872920/135‑1 (MQ=255)
                                                               cTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCa                                                                  <  1:1413004/139‑1 (MQ=255)
                                                                  gcgcGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAg                                                                                                                            >  2:1578312/1‑78 (MQ=255)
                                                                  gcgcGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAg                                                                                                                            <  1:1578312/78‑1 (MQ=255)
                                                                  gcgcGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGgctg                                                                           <  2:1463456/127‑1 (MQ=255)
                                                                  gcgcGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGgctg                                                                           >  1:1463456/1‑127 (MQ=255)
                                                                  gcgcGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCa                                                               >  1:1780946/1‑139 (MQ=255)
                                                                     cgTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATcc                                                            <  1:1620355/139‑1 (MQ=255)
                                                                        ggCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGAc                                                                                                                       <  1:1200608/77‑1 (MQ=255)
                                                                        ggCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGAc                                                                                                                       >  2:1200608/1‑77 (MQ=255)
                                                                           cacaTCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGACATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGa                                                      <  2:742033/139‑1 (MQ=255)
                                                                             catcatCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGaaa                                                    >  1:1817244/1‑139 (MQ=255)
                                                                                 atcaACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACa                                                <  1:208883/139‑1 (MQ=255)
                                                                                     acaaGGGTGCAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGTATCCAAGTGAAAAACAAAgg                                            <  1:1107468/139‑1 (MQ=255)
                                                                                      caaGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGt                                           >  1:1222205/1‑139 (MQ=255)
                                                                                        aGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCt                                                                                                                          <  1:1474522/58‑1 (MQ=255)
                                                                                        aGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCt                                                                                                                          >  2:1474522/1‑58 (MQ=255)
                                                                                        aGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTa                                                                                                               <  2:1665288/69‑1 (MQ=255)
                                                                                        aGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTa                                                                                                               >  1:1665288/1‑69 (MQ=255)
                                                                                        aGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGc                                                                                         <  2:523021/91‑1 (MQ=255)
                                                                                        aGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGc                                                                                         >  1:523021/1‑91 (MQ=255)
                                                                                        aGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAg                                         >  2:195552/1‑139 (MQ=255)
                                                                                         gggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGc                                                                                                                  >  1:1304249/1‑65 (MQ=255)
                                                                                         gggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGc                                                                                                                  <  2:1304249/65‑1 (MQ=255)
                                                                                         gggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACg                                                                                                             >  1:1185986/1‑70 (MQ=255)
                                                                                         gggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACg                                                                                                             <  2:1185986/70‑1 (MQ=255)
                                                                                          ggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGcc                                                                                                                                      >  1:186788/1‑44 (MQ=255)
                                                                                          ggTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGcc                                                                                                                                      <  2:186788/44‑1 (MQ=255)
                                                                                            tGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATc                                     <  1:621452/139‑1 (MQ=255)
                                                                                                gCAATCGGTGTGATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGc                                 <  2:1222205/139‑1 (MQ=255)
                                                                                                    tCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAg                             >  1:233368/1‑139 (MQ=255)
                                                                                                    tCGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAg                             >  1:348378/1‑139 (MQ=255)
                                                                                                     cGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTa                                                                                                                                 >  2:21457/1‑38 (MQ=255)
                                                                                                     cGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTa                                                                                                                                 <  1:21457/38‑1 (MQ=255)
                                                                                                     cGGTGTTATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGc                            >  1:148878/1‑139 (MQ=255)
                                                                                                           tATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCgg                                                                              <  1:123882/83‑1 (MQ=255)
                                                                                                           tATCGCGGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCgg                                                                              >  2:123882/1‑83 (MQ=255)
                                                                                                                cgGCACAGTCCATCGGTGAGCCGGGTACACAGCTGAc                                                                                                                       <  2:1426198/37‑1 (MQ=255)
                                                                                                                cgGCACAGTCCATCGGTGAGCCGGGTACACAGCTGAc                                                                                                                       >  1:1426198/1‑37 (MQ=255)
                                                                                                                cgGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCa                                                                                                                     <  2:1838072/39‑1 (MQ=255)
                                                                                                                cgGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCa                                                                                                                     >  1:1838072/1‑39 (MQ=255)
                                                                                                                cgGCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTc                 >  2:1496173/1‑139 (MQ=255)
                                                                                                                  gCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCg                                                                                                                 >  1:756229/1‑41 (MQ=255)
                                                                                                                  gCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCg                                                                                                                 <  2:756229/41‑1 (MQ=255)
                                                                                                                  gCACAGTCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCgg               >  1:412277/1‑139 (MQ=255)
                                                                                                                        tCCATCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGa         >  2:338453/1‑139 (MQ=255)
                                                                                                                        cccATCGGTGAGCCGGCTACGCAGCTGACCATGCGTACGTTCCACACCGGTGATGCGGCACCTGGTGCGGCTGCTGAATCCAGCATCATAGGGAAAAGCCGAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGa         >  1:585607/2‑139 (MQ=255)
                                                                                                                            tCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTc     <  2:1817244/139‑1 (MQ=255)
                                                                                                                            tCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTc     <  1:1873163/139‑1 (MQ=255)
                                                                                                                            tCGGTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTc     <  1:1496173/139‑1 (MQ=255)
                                                                                                                               gTGAGCCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAg  <  2:201412/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
GTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGGCGCGTGGCCACATCATCAACAAGGGTGAAGCAATCGGTGTTATCGCGGCACAGTCCATCGGTGAACCGGGTACACAGCTGACCATGCGTACGTTCCACATCGGTGGTGCGGCATCTCGTGCGGCTGCTGAATCCAGCATCCAAGTGAAAAACAAAGGTAGCATCAAGCTCAGCAACGTGAAGTCGGTTGTGAACTCCAG  >  NC_000913/4187993‑4188258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑