Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,232,752 G→A A161V (GCG→GTG)  lysC ← lysine‑sensitive aspartokinase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,232,7520GA100.0% 224.3 / NA 68A161V (GCG→GTG) lysClysine‑sensitive aspartokinase 3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/36);  total (32/36)

GTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGAC  >  NC_000913/4232623‑4232871
                                                                                                                                 |                                                                                                                       
gTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTatat                                                                                                                <  1:68594/139‑1 (MQ=255)
   taATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTg                                                                                                             >  1:264537/1‑139 (MQ=255)
               ccACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGAc                                                                                                 <  1:1749301/139‑1 (MQ=255)
                    gCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAAt                                                                                            >  1:481466/1‑139 (MQ=255)
                       aaGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTg                                                                                                       <  2:1527835/125‑1 (MQ=255)
                       aaGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTg                                                                                                       >  1:1527835/1‑125 (MQ=255)
                              gtTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTgg                                                                                  >  1:1069951/1‑139 (MQ=255)
                               ttGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGt                                                                                 <  1:1302139/139‑1 (MQ=255)
                                    cGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                            >  2:1238319/1‑139 (MQ=255)
                                    cGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                            <  1:1778636/139‑1 (MQ=255)
                                     gACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCAt                                                                           <  1:545035/139‑1 (MQ=255)
                                         tttATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt                                                                       <  1:267262/139‑1 (MQ=255)
                                         tttATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACt                                                                       <  1:1238319/139‑1 (MQ=255)
                                          ttATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACtt                                                                      >  2:1684975/1‑139 (MQ=255)
                                           tATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATc                                                                          >  2:1060405/1‑134 (MQ=255)
                                           tATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATc                                                                          <  1:1060405/134‑1 (MQ=255)
                                           tATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGACCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATc                                                                          >  2:591806/1‑134 (MQ=255)
                                           tATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGACCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATc                                                                          <  1:591806/134‑1 (MQ=255)
                                            aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATc                                                                                                               <  1:1669276/96‑1 (MQ=255)
                                            aTTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATc                                                                                                               >  2:1669276/1‑96 (MQ=255)
                                              tttCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                  <  2:1069951/139‑1 (MQ=255)
                                              tttCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                  <  2:1287628/139‑1 (MQ=255)
                                                 cGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTAc                                                               >  2:1209246/1‑139 (MQ=255)
                                                  gCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGATCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa                                                                                             >  1:1470407/1‑108 (MQ=255)
                                                  gCTACCGATAAAGCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGTGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa                                                                                             <  2:1470407/108‑1 (MQ=255)
                                                             aTCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACtg                                                   <  2:264537/139‑1 (MQ=255)
                                                                    ggTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCt                                                                                                                    <  2:705159/67‑1 (MQ=255)
                                                                    ggTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCt                                                                                                                    >  1:705159/1‑67 (MQ=255)
                                                                    ggTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg                                                                                          <  2:727659/93‑1 (MQ=255)
                                                                    ggTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCg                                                                                          >  1:727659/1‑93 (MQ=255)
                                                                         tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa                                                                                             >  2:118947/1‑85 (MQ=255)
                                                                         tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCaaa                                                                                             <  1:118947/85‑1 (MQ=255)
                                                                         tCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATc                                       >  1:42584/1‑139 (MQ=255)
                                                                          cACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                            <  1:322961/101‑1 (MQ=255)
                                                                          cACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCa                                                                            >  2:322961/1‑101 (MQ=255)
                                                                           aCTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                  >  2:727020/1‑110 (MQ=255)
                                                                           aCTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                  <  1:727020/110‑1 (MQ=255)
                                                                             tAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCTGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCACGt                                   <  1:1209246/139‑1 (MQ=255)
                                                                             tAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGt                                   <  1:1684975/139‑1 (MQ=255)
                                                                                    tCATTGAGACGTGGGAGCAGCTGTAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCa                            >  2:431774/1‑139 (MQ=255)
                                                                                    tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTgg                                                                                                            >  1:636014/1‑59 (MQ=255)
                                                                                    tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTgg                                                                                                            <  2:636014/59‑1 (MQ=255)
                                                                                    tCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCa                            >  1:1150551/1‑139 (MQ=255)
                                                                                     cATTGAGACGTGGGATCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATc                                                                                                               <  1:1241991/55‑1 (MQ=255)
                                                                                     cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATc                                                                                                               >  2:1241991/1‑55 (MQ=255)
                                                                                     cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTa                                                                <  1:1036185/102‑1 (MQ=255)
                                                                                     cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTa                                                                >  2:1036185/1‑102 (MQ=255)
                                                                                     cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                     >  2:1181745/1‑113 (MQ=255)
                                                                                     cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCAc                                                     <  1:1181745/113‑1 (MQ=255)
                                                                                     cATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAg                           >  1:984703/1‑139 (MQ=255)
                                                                                         gagaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtc                       <  2:42584/139‑1 (MQ=255)
                                                                                         gagaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtc                       >  1:1361984/1‑139 (MQ=255)
                                                                                          agaCGTGGGAGCAGCTGTAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtct                      <  1:431774/139‑1 (MQ=255)
                                                                                           gaCGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGAtctc                     <  2:984703/139‑1 (MQ=255)
                                                                                               tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGAc                                                                                                 <  2:1076161/59‑1 (MQ=255)
                                                                                               tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTg                                             >  1:1589305/1‑111 (MQ=255)
                                                                                               tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTg                                             <  2:1589305/111‑1 (MQ=255)
                                                                                               tGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTcaaca                 >  2:1408705/1‑139 (MQ=255)
                                                                                               tGGGAGCAGCTGCAGCGAGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGAc                                                                                                 >  1:1076161/1‑59 (MQ=255)
                                                                                                gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                  <  2:1777407/89‑1 (MQ=255)
                                                                                                gggAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACg                                                                  >  1:1777407/1‑89 (MQ=255)
                                                                                                      agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt                                                                                        >  1:1341110/1‑61 (MQ=255)
                                                                                                      agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGt                                                                                        <  2:1341110/61‑1 (MQ=255)
                                                                                                      agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATc                                                                          >  2:208460/1‑75 (MQ=255)
                                                                                                      agcTGCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATc                                                                          <  1:208460/75‑1 (MQ=255)
                                                                                                          gCAGCGCGGCCAGTTCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGt      <  2:481466/139‑1 (MQ=255)
                                                                                                                        tCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGAc  >  2:477777/1‑129 (MQ=255)
                                                                                                                        tCCGCCAGCACGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGAc  <  1:477777/129‑1 (MQ=255)
                                                                                                                                 |                                                                                                                       
GTATAATCGCTGCCTCCACGGCCAAGCGTCGTTGTACGACCTTTATTTTCGCTACCGATAAATCCCTGGGTGATCACTAAGCCTTCATTGAGACGTGGGAGCAGCTGCAGCGCGGCCAGTTCCGCCAGCGCGGCTATATCTGGCTCTGCACGACCAAATCGGTCGTTGGTACGCATCACTTTACGTACATCAAACCACTGTGCCTGAACATCGCGTTCGCGCAGGATCTCAACAAACAGCAGGGTCGAC  >  NC_000913/4232623‑4232871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑