Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,317,409	C→T	G163S (GGC→AGC)	phnL ←	ribophosphonate triphosphate synthase subunit, putative ABC transporter‑related ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,317,409	0	C	T	100.0%	147.2 / NA	45	G163S (GGC→AGC)	phnL	ribophosphonate triphosphate synthase subunit, putative ABC transporter‑related ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/21);  total (24/21)

GCCTACGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGC  >  NC_000913/4317284‑4317540                                                                                                                              |                                                                                                                                    gCCTACGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTg                                                                                                                        >  1:1642936/1‑139 (MQ=255)      cGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGAcgcg                                                                                                                   >  2:437585/1‑139 (MQ=255)           gCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCgctgct                                                                                                              >  2:1594748/1‑139 (MQ=255)           gCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCgctgct                                                                                                              >  2:713559/1‑139 (MQ=255)             tGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGt                                                                                                            >  1:368807/1‑139 (MQ=255)              gcgcCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCtgcg                                                                                                                                  <  1:129338/116‑1 (MQ=255)              gcgcCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCtgcg                                                                                                                                  >  2:129338/1‑116 (MQ=255)                 ccACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGc                                                                                                        >  1:1520624/1‑139 (MQ=255)                   aCGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCa                                                                                                      <  2:203339/139‑1 (MQ=255)                    cGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGt                                                                                                                          <  1:738139/118‑1 (MQ=255)                    cGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGt                                                                                                                          >  2:738139/1‑118 (MQ=255)                    cGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTAACGCGCTGCTGTTCGCCAc                                                                                                     >  2:543367/1‑139 (MQ=255)                          ttGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGaaa                                                                                               >  2:1754871/1‑139 (MQ=255)                            ggCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAAt                                                                                             >  2:1196712/1‑139 (MQ=255)                                 cgcgAATCAGTTCCACTACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGa                                                                                        >  2:1465673/1‑139 (MQ=255)                                 cgcgAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGa                                                                                        <  1:713559/139‑1 (MQ=255)                                      aTCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATggtg                                                                                   <  1:1754871/139‑1 (MQ=255)                                         aGTTCCACTACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATggtgcca                                                                                <  1:1465673/139‑1 (MQ=255)                                           ttCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATggtgccagg                                                                              <  1:437585/139‑1 (MQ=255)                                                    cgcgGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAgg                                                                     >  2:559886/1‑139 (MQ=255)                                                            tGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTAACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCgg                                                             <  1:543367/139‑1 (MQ=255)                                                                  tGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGtt                                                       <  2:1520624/139‑1 (MQ=255)                                                                        ccAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                           >  2:868679/1‑81 (MQ=255)                                                                        ccAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGtt                                                                                                           <  1:868679/81‑1 (MQ=255)                                                                        ccAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCg                                                 >  2:1680360/1‑139 (MQ=255)                                                                                            tCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCtt                             >  1:1794625/1‑139 (MQ=255)                                                                                                gcagcaGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgc                         >  2:803168/1‑139 (MQ=255)                                                                                                gcagcaGAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgc                         >  2:709603/1‑139 (MQ=255)                                                                                                    cagAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCAc                                                          >  2:32486/1‑102 (MQ=255)                                                                                                    cagAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCAc                                                          <  1:32486/102‑1 (MQ=255)                                                                                                    cagAATGGGGTAGTCGACGATAAAGCTACGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAgcggcg                     <  2:1031101/139‑1 (MQ=255)                                                                                                     agAATGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGgcgc                    <  2:1642936/139‑1 (MQ=255)                                                                                                         tGGGGTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGc                >  1:199114/1‑139 (MQ=255)                                                                                                            ggTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGa                                                                                        >  1:442665/1‑64 (MQ=255)                                                                                                            ggTAGTCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGa                                                                                        <  2:442665/64‑1 (MQ=255)                                                                                                                 tCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATggtgcca                                                                                >  1:862735/1‑67 (MQ=255)                                                                                                                 tCGACGATAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATggtgcca                                                                                <  2:862735/67‑1 (MQ=255)                                                                                                                  cgacgaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCgg       <  1:559886/139‑1 (MQ=255)                                                                                                                  cgacgaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCgg       <  2:368807/139‑1 (MQ=255)                                                                                                                   gacgaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAgg                                                   >  1:1002028/1‑94 (MQ=255)                                                                                                                   gacgaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAgg                                                   <  2:1002028/94‑1 (MQ=255)                                                                                                                   gacgaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGa      >  2:1407966/1‑139 (MQ=255)                                                                                                                    acgaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGaa     <  1:677725/139‑1 (MQ=255)                                                                                                                      gaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGtt                                                       >  2:1556045/1‑87 (MQ=255)                                                                                                                      gaTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGtt                                                       <  1:1556045/87‑1 (MQ=255)                                                                                                                       aTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGc  <  1:1680360/139‑1 (MQ=255)                                                                                                                       aTAAAGCTGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGc  <  1:1407966/139‑1 (MQ=255)                                                                                                                              |                                                                                                                                    GCCTACGATGGCTGCGCCACGGGTTTTGGCTTCGCGAATCAGTTCCACCACCGCGGCGCTGTTTTTGGCGTCCAGCGAGGCGGTAGGTTCGTCAAGCAGCAGAATGGGGTAGTCGACGATAAAGCCGCGGGCGATGTTGACGCGCTGCTGTTCGCCACCGGAAAATGTCGATGGTGCCAGGTGCCACAGGCGTTCCGGCACGTTCAGGCGGGTGAGAAGACGCGCGGCTTTAGCGGCGCAGGCTTCACGCGGAACGC  >  NC_000913/4317284‑4317540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑