Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,428,079	C→T	pseudogene (386/402 nt) *213* (TAG→TAA)	ytfA → ytfB ←	pseudogene, related to transcriptional regulators OapA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,428,079	0	C	T	98.7%	248.7 / ‑4.4	77	pseudogene (386/402 nt) *213* (TAG→TAA)	ytfA ytfB	pseudogene, related to transcriptional regulators OapA family protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (37/39);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

ATATTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGTT  >  NC_000913/4427948‑4428207                                                                                                                                    |                                                                                                                                 atatTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACcgcg                                                                                                                           >  1:858176/1‑139 (MQ=255) atatTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACcgcg                                                                                                                           >  2:539419/1‑139 (MQ=255)    ttattTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGG‑AGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACg                                                                                                                       <  2:1519418/139‑1 (MQ=255)               aCCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTg                                                                                                             >  1:1391596/1‑139 (MQ=255)                      tACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGCAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCg                                                                                                      >  1:1299229/1‑139 (MQ=255)                           ttGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCgcgc                                                                                                                          <  1:1796754/114‑1 (MQ=255)                           ttGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCgcgc                                                                                                                          >  2:1796754/1‑114 (MQ=255)                             ggCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCg                                                                                               >  2:966046/1‑139 (MQ=255)                             ggCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCg                                                                                               >  1:949188/1‑139 (MQ=255)                             ggCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCg                                                                                               >  2:317867/1‑139 (MQ=255)                              gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              <  1:1468385/139‑1 (MQ=255)                              gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              >  2:230069/1‑139 (MQ=255)                              gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              >  2:1574315/1‑139 (MQ=255)                              gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              >  2:1358477/1‑139 (MQ=255)                               ccTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCggg                                                                                             <  1:311619/139‑1 (MQ=255)                               ccTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCggg                                                                                             <  1:356709/139‑1 (MQ=255)                                  ggTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGa                                                                                          <  2:1260190/139‑1 (MQ=255)                                   gTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGaa                                                                                         >  2:708265/1‑139 (MQ=255)                                    tttATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGCAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAAc                                                                                        <  2:1299229/139‑1 (MQ=255)                                      tATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAg                                                                                      <  1:1358477/139‑1 (MQ=255)                                      tATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAg                                                                                      <  2:1324146/139‑1 (MQ=255)                                       aTCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAgg                                                                                     >  2:420983/1‑139 (MQ=255)                                         cacaTCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCgcgc                                                                                                                          >  2:1084709/1‑100 (MQ=255)                                         cacaTCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCgcgc                                                                                                                          <  1:1084709/100‑1 (MQ=255)                                         cacaTCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                   <  1:708265/139‑1 (MQ=255)                                           caTCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCt                                                                                 <  1:539419/139‑1 (MQ=255)                                             tCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGt                                                                               <  1:966046/139‑1 (MQ=255)                                             tCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGt                                                                               <  1:317867/139‑1 (MQ=255)                                                 tATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGtt                                                                              >  1:1278366/1‑136 (MQ=255)                                                 tATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGat                                                                           <  1:420983/139‑1 (MQ=255)                                                 tATGGTTTCACCCCAGGCACTAGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCAGCTGGCGGGTGAACAGGACCTGtt                                                                              <  2:1278366/136‑1 (MQ=255)                                                 tATGGTTTCACCCCACGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGat                                                                           >  1:1257279/1‑139 (MQ=255)                                                      tttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTc                                                                      >  2:510178/1‑139 (MQ=255)                                                        tCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTccc                                                                    <  2:1664724/139‑1 (MQ=255)                                                         ccccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTcccc                                                                   >  1:1855012/3‑139 (MQ=255)                                                         cACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTcccc                                                                   <  2:1391596/139‑1 (MQ=255)                                                          aCCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCg                                                                  >  2:94429/1‑139 (MQ=255)                                                           ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              >  1:1670280/1‑110 (MQ=255)                                                           ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              <  2:1670280/110‑1 (MQ=255)                                                           ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGt                                                                 >  1:539587/1‑139 (MQ=255)                                                              cAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATc                                                              <  1:230069/139‑1 (MQ=255)                                                               aGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCa                                                             <  1:94429/139‑1 (MQ=255)                                                                ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCaa                                                            >  2:964707/1‑139 (MQ=255)                                                                      ggAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTg                                                                                                             <  1:1294672/84‑1 (MQ=255)                                                                      ggAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTg                                                                                                             >  2:1294672/1‑84 (MQ=255)                                                                      ggAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              >  2:459169/1‑99 (MQ=255)                                                                      ggAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              <  1:459169/99‑1 (MQ=255)                                                                      ggAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTa                                                      <  1:510178/139‑1 (MQ=255)                                                                      ggAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGC‑TTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTaa                                                     >  2:1507568/1‑139 (MQ=255)                                                                                  tAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTcacca                                          <  2:858176/139‑1 (MQ=255)                                                                                     ggTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                   <  1:1062835/95‑1 (MQ=255)                                                                                     ggTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                   >  2:1062835/1‑95 (MQ=255)                                                                                     ggTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCAcgc                                       <  1:91241/139‑1 (MQ=255)                                                                                         atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCgcgc                                                                                                                          >  2:436035/1‑52 (MQ=255)                                                                                         atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCgcgc                                                                                                                          <  1:436035/52‑1 (MQ=255)                                                                                         atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTccc                                                                    <  2:1743025/106‑1 (MQ=255)                                                                                         atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTccc                                                                    >  1:1743025/1‑106 (MQ=255)                                                                                          tcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              >  1:338735/1‑79 (MQ=255)                                                                                          tcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              <  2:338735/79‑1 (MQ=255)                                                                                          tcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAAt                                                           >  2:879153/1‑114 (MQ=255)                                                                                          tcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAAt                                                           <  1:879153/114‑1 (MQ=255)                                                                                           caAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                   <  1:876149/89‑1 (MQ=255)                                                                                           caAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                   >  2:876149/1‑89 (MQ=255)                                                                                                  aCAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTg                          >  2:691811/1‑139 (MQ=255)                                                                                                    aaTCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACCGGACCt                                                                                 >  1:1210366/1‑82 (MQ=255)                                                                                                    aaTCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCt                                                                                 <  2:1210366/82‑1 (MQ=255)                                                                                                            gCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAAc                                                                                        <  2:1543091/67‑1 (MQ=255)                                                                                                            gCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAAc                                                                                        >  1:1543091/1‑67 (MQ=255)                                                                                                                gCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              >  1:331617/1‑57 (MQ=255)                                                                                                                gCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              <  2:331617/57‑1 (MQ=255)                                                                                                                 cTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              >  1:375541/1‑56 (MQ=255)                                                                                                                 cTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                              <  2:375541/56‑1 (MQ=255)                                                                                                                          gACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                   >  1:1249692/1‑58 (MQ=255)                                                                                                                          gACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                   <  2:1249692/58‑1 (MQ=255)                                                                                                                          gACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTAt                                                               >  2:443717/1‑78 (MQ=255)                                                                                                                          gACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTAt                                                               <  1:443717/78‑1 (MQ=255)                                                                                                                          gACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGtt  <  1:964707/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 ATATTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGTT  >  NC_000913/4427948‑4428207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑