Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,519,014 G→A pseudogene (294/504 nt) insB1 → IS1 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,519,0140GA100.0% 370.3 / NA 127pseudogene (294/504 nt)insB1IS1 transposase B,IS, phage, Tn, Transposon‑related functions, extrachromosomal, transposon related
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (67/60);  total (67/60)

Only 100 of 127 total aligned reads displayed.

CGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGAAGTTAC  >  NC_000913/4518883‑4519150
                                                                                                                                   |                                                                                                                                        
cGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACgg                                                                                                                                   >  2:1490294/1‑139 (MQ=255)
                         aTGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACg                                                                                                          >  1:5434/1‑139 (MQ=255)
                           gACAGGCTCCGGAAGACGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCt                                                                                                       <  2:1453867/139‑1 (MQ=11)
                                gATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCt                                                                                                       <  1:1262507/135‑1 (MQ=255)
                                gATACGGAGAACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCt                                                                                                       >  2:1262507/1‑135 (MQ=255)
                                 ctccGGAAGACGGTTGTTGCGCACGTATTCGGTGAACTCACTAAGGAGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAATTGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAggg                                                                                                 >  2:1257885/4‑139 (MQ=11)
                                 aTACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAgg                                                                                                  >  1:1312534/1‑139 (MQ=255)
                                   aCGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGa                                                                                                >  2:535901/1‑139 (MQ=255)
                                     ggaagaCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACg                                                                                                                                    >  1:744456/1‑100 (MQ=11)
                                     ggaagaCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACg                                                                                                                                    <  2:744456/100‑1 (MQ=11)
                                      gaagaCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGc                                                                                            <  2:957605/136‑1 (MQ=14)
                                      gaagaCGGTTGTTGCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGc                                                                                            >  2:614157/4‑139 (MQ=14)
                                          aCGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTa                                                                                                                   >  1:72133/1‑113 (MQ=255)
                                              ttgttgCGCACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGta                                                                                    <  2:1252325/139‑2 (MQ=14)
                                                gttgcgcACGTATTCGGTGAACGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAAt                                                                                  <  2:506570/136‑1 (MQ=11)
                                                gtgGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTa                                                                                   >  1:1857315/1‑139 (MQ=255)
                                                gtgGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTa                                                                                   >  2:1131637/1‑139 (MQ=255)
                                                gtgGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTa                                                                                   >  1:1353394/1‑139 (MQ=255)
                                                     gcACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                                                              <  1:1538417/139‑1 (MQ=255)
                                                      cACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                                                                        <  1:1148148/128‑1 (MQ=255)
                                                      cACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                                                                        >  2:1148148/1‑128 (MQ=255)
                                                       aCGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                                                            <  2:1353394/139‑1 (MQ=255)
                                                       aCGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                                                            <  2:1857315/139‑1 (MQ=255)
                                                        cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTTAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAg                                                                           >  1:1108820/1‑139 (MQ=255)
                                                        cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCt                                                                                                       <  2:1275695/111‑1 (MQ=255)
                                                        cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                                                            <  1:1038238/138‑1 (MQ=255)
                                                        cGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                                                            >  2:1038238/1‑138 (MQ=255)
                                                            ttCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTACGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGc                                                                              <  1:1021540/132‑1 (MQ=255)
                                                             tCGGTGAACGGACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGATat                                                                     <  1:1257885/139‑2 (MQ=14)
                                                              cGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTac                                                                     >  2:1178119/1‑139 (MQ=255)
                                                                   aaCGCACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCgatatacgcag                                                               >  1:319386/1‑129 (MQ=11)
                                                                     cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                                                         >  2:788177/1‑80 (MQ=255)
                                                                     cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGcc                                                                                                                         <  1:788177/80‑1 (MQ=255)
                                                                     cGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAgc                                                              >  1:1679073/1‑139 (MQ=255)
                                                                       cACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCt                                                                                                       >  1:923420/1‑95 (MQ=17)
                                                                       cACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCt                                                                                                       <  2:923420/95‑1 (MQ=17)
                                                                       cACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCgatatac                                                                   >  1:86435/1‑125 (MQ=14)
                                                                       cACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCgatatac                                                                   <  2:86435/131‑7 (MQ=14)
                                                                       cACTATGGCGACGCTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAgcgaa                                                           <  1:614157/139‑3 (MQ=11)
                                                                           ctGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCa                                                                                                            >  2:1092460/1‑87 (MQ=255)
                                                                           atGGCGACGCAGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa                                                       <  1:1408508/138‑1 (MQ=11)
                                                                            tGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGa                                                       <  2:1434045/139‑1 (MQ=255)
                                                                               cgacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa                                                       <  1:1455840/130‑1 (MQ=11)
                                                                               cgacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGa                                                       >  2:1455840/6‑135 (MQ=14)
                                                                                gacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggt                                                  >  1:1304711/5‑137 (MQ=11)
                                                                                gacgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggt                                                  >  1:878907/5‑137 (MQ=11)
                                                                                  cgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCgatat                                                                     <  2:1234348/116‑5 (MQ=17)
                                                                                  cgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggtat                                                <  2:319386/137‑5 (MQ=11)
                                                                                  cgcTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCggtat                                                >  2:686221/3‑135 (MQ=11)
                                                                                    cTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGTATaa                                              >  2:698611/1‑139 (MQ=11)
                                                                                    cTGGAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGTATaa                                              >  1:1591992/1‑139 (MQ=11)
                                                                                      ggAGCGTCTTCTGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                        <  2:175414/63‑1 (MQ=37)
                                                                                        aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACa                                                 >  1:326532/1‑133 (MQ=255)
                                                                                        aGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACa                                                 <  2:326532/133‑1 (MQ=255)
                                                                                            tcttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGTGAAAGCTGCACGTTATCAGCAAGCGTTacac                                                                   <  1:1246637/111‑1 (MQ=255)
                                                                                            tcttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatctgaat                                       >  2:1239044/1‑139 (MQ=255)
                                                                                            tcttctGAGCCTGCTGTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAg                                                                                             >  1:1267093/1‑84 (MQ=21)
                                                                                             cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                          <  2:395159/55‑1 (MQ=255)
                                                                                             cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGAtggctggc                                                                                                                          >  1:395159/1‑55 (MQ=255)
                                                                                             cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAggg                                                                                                 >  1:18274/1‑80 (MQ=255)
                                                                                             cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGaa                                                                                               >  1:499429/1‑82 (MQ=255)
                                                                                             cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                                                                        <  1:1543324/89‑1 (MQ=255)
                                                                                             cttctGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                                                                        >  2:1543324/1‑89 (MQ=255)
                                                                                             cttctGAGCCTGCTGTCGGCCTCTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                        <  1:758684/57‑1 (MQ=37)
                                                                                             cttctGAGCCTGCTGTCGGCCTCTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                        >  2:758684/1‑57 (MQ=37)
                                                                                                 tGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCaa                                                                            >  1:623925/1‑97 (MQ=255)
                                                                                                   aGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTc                                                                 >  2:1033918/1‑106 (MQ=255)
                                                                                                   aGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTc                                                                 <  1:1033918/106‑1 (MQ=255)
                                                                                                   aGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGac                                <  1:1537987/139‑1 (MQ=255)
                                                                                                      ctgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                        >  2:30940/1‑48 (MQ=255)
                                                                                                      ctgctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                        <  1:30940/48‑1 (MQ=255)
                                                                                                       tgctgTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGc                                                                          >  2:1223840/1‑92 (MQ=17)
                                                                                                       tgctgTGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGc                                                                          <  1:1223840/92‑1 (MQ=17)
                                                                                                        gctgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACAt                           <  2:1584048/139‑1 (MQ=255)
                                                                                                         gctgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAg                                                      >  2:1640533/6‑111 (MQ=11)
                                                                                                         gctgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCAAGCGATATACGCAGCGAATTGAg                                                      <  1:1640533/106‑1 (MQ=11)
                                                                                                          tgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatct                                           >  2:1476374/1‑121 (MQ=255)
                                                                                                          tgTCGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatct                                           <  1:1476374/121‑1 (MQ=255)
                                                                                                          ctgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                        >  1:1451122/5‑44 (MQ=25)
                                                                                                          ctgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACg                                                                                      <  1:1538569/74‑1 (MQ=18)
                                                                                                          ctgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                                            <  1:1022403/84‑1 (MQ=17)
                                                                                                          ctgtggCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTAATCAGCaa                                                                            >  2:1022403/5‑88 (MQ=17)
                                                                                                             cGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                        <  1:241540/41‑1 (MQ=255)
                                                                                                             cGGCCTTTGAGGTCGTGGTATAAATGACGGATGGCTGGCCg                                                                                                                        >  2:241540/1‑41 (MQ=255)
                                                                                                              ggCCTTTGAGGTCGTGGTATAAATGACCGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAGTCTGAATCTGAGACAACATCTCGCa                     >  2:1215031/1‑139 (MQ=255)
                                                                                                                   ttGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCt                >  1:506140/1‑139 (MQ=255)
                                                                                                                   ttGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCt                <  1:1131637/139‑1 (MQ=255)
                                                                                                                    tGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                                          <  2:772874/64‑1 (MQ=255)
                                                                                                                    tGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCa                                                           >  1:512820/1‑95 (MQ=255)
                                                                                                                    tGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCa                                                           <  2:512820/95‑1 (MQ=255)
                                                                                                                    tGAGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTg               >  1:722179/1‑139 (MQ=255)
                                                                                                                      aGGTCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTggg             >  2:577934/1‑139 (MQ=255)
                                                                                                                         tCGTGGTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACg          <  2:1312534/139‑1 (MQ=255)
                                                                                                                             ggTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAg                                                                                             <  2:703564/52‑1 (MQ=255)
                                                                                                                             ggTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCa                                                                                        >  2:567360/1‑57 (MQ=255)
                                                                                                                              gTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                                          >  1:226142/1‑54 (MQ=255)
                                                                                                                              gTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTg                                                                                          <  2:226142/54‑1 (MQ=255)
                                                                                                                              gTATAAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGAAGt     <  2:585730/139‑1 (MQ=255)
                                                                                                                                ataAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatctgaatc                                      <  1:683815/104‑1 (MQ=255)
                                                                                                                                ataAATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCAGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATaatctgaatc                                      >  2:683815/1‑104 (MQ=255)
                                                                                                                                   |                                                                                                                                        
CGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTGGCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATAGATGACGGATGGCTGGCCGCTGTATGAATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGCGCATTGAGCGACATAATCTGAATCTGAGACAACATCTGGCAAGGCTGGGACGGAAGTTAC  >  NC_000913/4518883‑4519150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: