Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	379,606	C→T	*92* (TAG→TAA)	frmR ←	regulator protein that represses frmRAB operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	379,606	0	C	T	100.0%	184.0 / NA	57	*92* (TAG→TAA)	frmR	regulator protein that represses frmRAB operon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

CTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCG  >  NC_000913/379472‑379741                                                                                                                                       |                                                                                                                                        cTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTAtt                                                                                                                                     >  2:338998/1‑139 (MQ=255)      aTTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGa                                                                                                                                <  2:885438/139‑1 (MQ=255)      aTTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACAATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGa                                                                                                                                >  1:1334484/1‑139 (MQ=255)      aTTAGAACTTCACCATTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCTGGGACAAATGCAACGGCAGCACGTAATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGACTCAGTTATTTAAGa                                                                                                                                <  2:1151548/139‑1 (MQ=255)           cACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGc                                                                                                                           >  1:660246/1‑139 (MQ=255)           cACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGc                                                                                                                           >  2:396415/1‑139 (MQ=255)           cACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCACTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGc                                                                                                                           >  1:14910/1‑139 (MQ=255)            aCTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCa                                                                                                                          <  1:923487/139‑1 (MQ=255)                 aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     <  2:943784/139‑1 (MQ=255)                 aCCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     <  2:660246/139‑1 (MQ=255)                 aCCTTTTTTCGGGGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     <  1:396415/139‑1 (MQ=255)                    ttttttCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAg                                                                                                                  <  1:338998/139‑1 (MQ=255)                      ttttCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTGAGTTATTTAAGATAGGCACGAACCAGtt                                                                                                                >  2:1127831/1‑139 (MQ=255)                        ttCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCa                                                                                                              <  1:904868/139‑1 (MQ=255)                             tggtgCAACTTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCACCTCGCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGa                                                                                                                       <  1:1418250/125‑1 (MQ=255)                             tggtgCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGa                                                                                                                       >  2:1418250/1‑125 (MQ=255)                                       tCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     >  2:620636/1‑117 (MQ=255)                                       tCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     <  1:620636/117‑1 (MQ=255)                                       tCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACg                                                                                               >  1:1636635/1‑139 (MQ=255)                                       tCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACg                                                                                               <  2:288348/139‑1 (MQ=255)                                             tCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTgg                                                                                         <  1:1127831/139‑1 (MQ=255)                                                 cGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGa                                                                                     >  2:280107/1‑139 (MQ=255)                                                   aTTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACt                                                                                   >  2:168411/1‑139 (MQ=255)                                                       ccAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcgc                                                                               <  1:280107/139‑1 (MQ=255)                                                         aGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCACTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCgg                                                                             <  2:14910/139‑1 (MQ=255)                                                                tACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGc                                                                      >  2:1874734/1‑139 (MQ=255)                                                                        gCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCaa                                                                                                 >  2:1550694/1‑104 (MQ=255)                                                                        gCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCaa                                                                                                 <  1:1550694/104‑1 (MQ=255)                                                                        gCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCAttt                                                              >  1:1833952/1‑139 (MQ=255)                                                                            aTGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGAATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGt                                                          <  2:1334484/139‑1 (MQ=255)                                                                                 aCGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAAc                                                     <  2:116499/139‑1 (MQ=255)                                                                                 aCGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAAc                                                     >  2:191135/1‑139 (MQ=255)                                                                                  cGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACg                                                    <  2:1636635/139‑1 (MQ=255)                                                                                      agcaCGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTc                                                <  1:191135/139‑1 (MQ=255)                                                                                              aTTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGatat                                        <  2:1434032/139‑1 (MQ=255)                                                                                                  cATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCt                                    >  2:176516/1‑139 (MQ=255)                                                                                                        tcGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAg                              >  2:1269617/1‑139 (MQ=255)                                                                                                        tcGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTC‑AACGTTTCCCGGATATGGCTTTCAAGc                             >  2:1066331/1‑139 (MQ=255)                                                                                                         cGCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGc                             >  1:691269/1‑139 (MQ=255)                                                                                                          gCTCTTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCa                            <  2:691269/139‑1 (MQ=255)                                                                                                           ctctTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     >  1:1884699/1‑49 (MQ=255)                                                                                                           ctctTCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     <  2:1884699/49‑1 (MQ=255)                                                                                                               tCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     <  2:125692/45‑1 (MQ=255)                                                                                                               tCCTCAATATGGTAATAGATTCAGTTATTTAAGATAGGCACGAAc                                                                                                                     >  1:125692/1‑45 (MQ=255)                                                                                                                      tatGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTc                                                                                 <  2:95438/74‑1 (MQ=255)                                                                                                                      tatGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTc                                                                                 >  1:95438/1‑74 (MQ=255)                                                                                                                      tatGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATgg                                      >  2:294182/1‑117 (MQ=255)                                                                                                                      tatGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATgg                                      <  1:294182/117‑1 (MQ=255)                                                                                                                      tatGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCa                >  2:1448945/1‑139 (MQ=255)                                                                                                                        tGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAg                                                                    <  2:1038023/85‑1 (MQ=255)                                                                                                                        tGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAg                                                                    >  1:1038023/1‑85 (MQ=255)                                                                                                                        tGGTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGc              >  1:1167032/1‑139 (MQ=255)                                                                                                                          gTAATAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCACGGATATGGCTTTCAAGCACTTCTGCCATCAGccc            >  2:1133577/1‑139 (MQ=255)                                                                                                                              tAGATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTa        >  1:1714156/1‑139 (MQ=255)                                                                                                                                gATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcgc                                                                               >  2:1532407/1‑66 (MQ=255)                                                                                                                                gATTCAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTcgc                                                                               <  1:1532407/66‑1 (MQ=255)                                                                                                                                    cAGTTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCcgcg  <  1:1874734/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        CTTTAATTAGCACTTCACCTTTTTTCGGTGGTGCAACGTCAATTTCAACGATTTCCAGCGGTTTACCGGGAGCAAATGCAACGGCAGCACGTGATTTCATCTCTCGCTCTTCCTCAATATGGTAATAGATTCAGCTATTTAAGATAGGCACGAACCAGTTCAATAGTGTCGTCAACGGATTGGCTGACTTCGCGGCTGTAGCAGTCATTTCGGTCAAACGTTTCCCGGATATGGCTTTCAAGCACTTCTGCCATCAGCCCATTAGCCGCG  >  NC_000913/379472‑379741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑