Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	443,051	C→T	*197* (TAG→TAA)	yajL ←	oxidative‑stress‑resistance chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	443,051	0	C	T	100.0%	192.1 / NA	59	*197* (TAG→TAA)	yajL	oxidative‑stress‑resistance chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/30);  total (29/30)

GGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGT  >  NC_000913/442923‑443182                                                                                                                                 |                                                                                                                                    ggggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           <  1:1165267/139‑1 (MQ=255) ggggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           >  1:1706978/1‑139 (MQ=255) ggggTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           <  1:1613911/139‑1 (MQ=255)      gatgaGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATta                                                                                                                      >  1:1017513/1‑139 (MQ=255)              gTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact                                                                                                                                 <  1:1185403/120‑1 (MQ=255)              gTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact                                                                                                                                 >  2:1185403/1‑120 (MQ=255)                 tGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact                                                                                                                                 >  1:1565658/1‑117 (MQ=255)                 tGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTAGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact                                                                                                                                 <  2:1565658/117‑1 (MQ=255)                   cAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTgccg                                                                                                         <  1:531756/139‑1 (MQ=255)                                  cgcgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTtgtg                                                                                          >  2:1242939/1‑139 (MQ=255)                                    cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata                                                                                                                          >  2:785948/1‑105 (MQ=255)                                    cgAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata                                                                                                                          <  1:785948/105‑1 (MQ=255)                                      aGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGc                                                                                      >  2:904876/1‑139 (MQ=255)                                       gCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                                                                                     <  2:1706978/139‑1 (MQ=255)                                       gCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGcc                                                                                     <  1:624618/139‑1 (MQ=255)                                        cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           >  2:613452/1‑100 (MQ=255)                                        cAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           <  1:613452/100‑1 (MQ=255)                                           ggCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACtt                                                                                 <  1:904876/139‑1 (MQ=255)                                                      tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                              <  2:1577087/115‑1 (MQ=255)                                                      tgtgAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                              >  1:1577087/1‑115 (MQ=255)                                                          acggTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGGCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCAc                                                                  >  1:1128202/3‑139 (MQ=255)                                                          aaGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCaa                                                                  <  2:1628404/139‑2 (MQ=255)                                                            ggTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc                                                                <  2:1533655/139‑1 (MQ=255)                                                            ggTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc                                                                <  2:1017513/139‑1 (MQ=255)                                                                tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattac                                                                                                                                  >  1:378856/1‑69 (MQ=255)                                                                tatCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattac                                                                                                                                  <  2:378856/69‑1 (MQ=255)                                                                 atCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaac                                                           >  1:1487362/1‑139 (MQ=255)                                                                  tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaa                                                                                                                            <  1:1068673/73‑1 (MQ=255)                                                                  tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaa                                                                                                                            >  2:1068673/1‑73 (MQ=255)                                                                  tCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCCTTTTCACGCccaacc                                                          >  2:1252022/1‑139 (MQ=255)                                                                    gCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCccaaccaa                                                        >  1:765525/1‑139 (MQ=255)                                                                     cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCAt                                                                                                     >  2:816261/1‑93 (MQ=255)                                                                     cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCAt                                                                                                     <  1:816261/93‑1 (MQ=255)                                                                     cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                              >  2:723493/1‑100 (MQ=255)                                                                     cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGt                                                                                              <  1:723493/100‑1 (MQ=255)                                                                     cccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCcaaccaac                                                       >  2:472601/1‑139 (MQ=255)                                                                     cccGTAATTTGTGGTTTTTACGTCGCATATGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCcaaccaac                                                       <  2:1128202/139‑1 (MQ=255)                                                                      ccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGTCATCACCAGTTGTGAt                                                                                        >  1:1745327/1‑105 (MQ=255)                                                                      ccGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGTCATCACCAGTTGTGAt                                                                                        <  2:1745327/105‑1 (MQ=255)                                                                       cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATtata                                                                                                                    <  2:485818/76‑1 (MQ=255)                                                                       cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATtata                                                                                                                    >  1:485818/1‑76 (MQ=255)                                                                       cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc                                                                <  1:281928/128‑1 (MQ=255)                                                                       cGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGc                                                                >  2:281928/1‑128 (MQ=255)                                                                        gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact                                                                                                                                 <  1:1572626/62‑1 (MQ=255)                                                                        gTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACattact                                                                                                                                 >  2:1572626/1‑62 (MQ=255)                                                                                    ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           <  2:285485/56‑1 (MQ=255)                                                                                    ttttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           >  1:285485/1‑56 (MQ=255)                                                                                      ttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggg                                                                           <  1:1315339/102‑1 (MQ=255)                                                                                      ttACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggg                                                                           >  2:1315339/1‑102 (MQ=255)                                                                                       tACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata                                                                                                                          >  1:788336/1‑54 (MQ=255)                                                                                       tACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaata                                                                                                                          <  2:788336/54‑1 (MQ=255)                                                                                       tACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           >  2:1883265/1‑53 (MQ=255)                                                                                       tACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGtaat                                                                                                                           <  1:1883265/53‑1 (MQ=255)                                                                                             gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCa                                                                               >  1:1736324/1‑91 (MQ=255)                                                                                             gCATCTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCa                                                                               <  2:1736324/91‑1 (MQ=255)                                                                                                 cTGGTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGa                           <  2:765525/139‑1 (MQ=255)                                                                                                    gTCAGATGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAg                        >  1:549565/1‑139 (MQ=255)                                                                                                         aTGCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTa                   >  2:1744989/1‑139 (MQ=255)                                                                                                           gCGACGTTTGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTAcc                 <  1:472601/139‑1 (MQ=255)                                                                                                                   tGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCa                                                                                                <  2:173252/52‑1 (MQ=255)                                                                                                                   tGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCa                                                                                                >  1:173252/1‑52 (MQ=255)                                                                                                                   tGCCGCATCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCct         >  1:558924/1‑139 (MQ=255)                                                                                                                          tCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATa                                              <  1:1290316/95‑1 (MQ=255)                                                                                                                          tCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATa                                              >  2:1290316/1‑95 (MQ=255)                                                                                                                          tCCGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGt  <  2:1487362/139‑1 (MQ=255)                                                                                                                           ccGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggg                                                                           >  2:510718/1‑65 (MQ=255)                                                                                                                           ccGACATTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATggg                                                                           <  1:510718/65‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    GGGGTGATGAGCCGTCTGCAGGCGCTCTATCTGCGCGAGCAGGGCTTTAACAATGTGAAGGTATATCGCCCGTAATTTGTGGTTTTTACGTCGCATCTGGTCAGATGCGACGTTTGCCGCATCCGACACTACTCGTAATAATTATAAATCCCTGCCGCCATCACCAGTTGTGATGCCACTTCATGGGCTTTTTCACGCCCAACCAACAGGTCGATAATTTTCAGACCAAAGTCGATAGCTGTACCCGGCCCCTGGCTGGT  >  NC_000913/442923‑443182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑