Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	444,641	C→T	intergenic (‑126/‑42)	panE ← / → yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	444,641	0	C	T	100.0%	213.7 / NA	67	intergenic (‑126/‑42)	panE/yajQ	2‑dehydropantoate reductase, NADPH‑specific/phage Phi6 host factor, ATP/GTP binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/30);  total (37/30)

CTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGT  >  NC_000913/444521‑444764                                                                                                                         |                                                                                                                            ctcctcACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCAt                                                                                                           <  1:1668206/139‑1 (MQ=255)        cccGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAAt                                                                                                    >  2:1446564/1‑139 (MQ=255)           gCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGaa                                                                                                 <  1:1446564/139‑1 (MQ=255)                  cTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGaa                                                                                                 >  1:1601973/1‑132 (MQ=255)                  cTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGaa                                                                                                 <  2:1601973/132‑1 (MQ=255)                           ttCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGta                                                                                                                        >  2:1070894/1‑100 (MQ=255)                           ttCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGta                                                                                                                        <  1:1070894/100‑1 (MQ=255)                             cAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                               <  1:1508905/139‑1 (MQ=255)                                        tCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGata                                                                    >  1:1676748/1‑139 (MQ=255)                                        tCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGata                                                                    >  2:207371/1‑139 (MQ=255)                                         cccTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                   <  2:1294171/139‑1 (MQ=255)                                         cccTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                   <  1:332831/139‑1 (MQ=255)                                              aaTTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtct                                                              >  1:46047/1‑139 (MQ=255)                                                  gtgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCaaaa                                                                                                      <  1:1243216/95‑1 (MQ=255)                                                  gtgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTAATTAACTTTGTGGGTATTATGCTTCGCATCaaaa                                                                                                      >  2:1243216/1‑95 (MQ=255)                                                   tgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGc                                                                                <  2:1748918/116‑1 (MQ=255)                                                   tgCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGc                                                                                >  1:1748918/1‑116 (MQ=255)                                                    gCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAg                                                        >  1:626811/1‑139 (MQ=255)                                                    gCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAg                                                        <  1:207371/139‑1 (MQ=255)                                                     cATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                       >  2:864589/1‑139 (MQ=255)                                                     cATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGt                                                       >  1:105979/1‑139 (MQ=255)                                                      aTGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGtt                                                      <  2:105979/139‑1 (MQ=255)                                                          tGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAg                                                        <  2:358526/133‑1 (MQ=255)                                                          tGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAg                                                        >  1:358526/1‑133 (MQ=255)                                                                      gCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCa                                                                                                         <  2:468090/72‑1 (MQ=255)                                                                      gCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCa                                                                                                         >  1:468090/1‑72 (MQ=255)                                                                         caATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTa                                   <  2:1676748/139‑1 (MQ=255)                                                                            ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                   >  1:1054983/1‑104 (MQ=255)                                                                            ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                   <  2:1054983/104‑1 (MQ=255)                                                                            ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAAcg                                >  1:321244/1‑139 (MQ=255)                                                                            ttAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAAcg                                >  2:50412/1‑139 (MQ=255)                                                                               aGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCATAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCgg                             >  1:1225491/1‑139 (MQ=255)                                                                               aGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCgg                             >  2:1868730/1‑139 (MQ=255)                                                                                 ggATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                               >  2:636040/1‑87 (MQ=255)                                                                                 ggATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                               <  1:636040/87‑1 (MQ=255)                                                                                    tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                               <  1:1140745/84‑1 (MQ=255)                                                                                    tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                               >  2:1140745/1‑84 (MQ=255)                                                                                    tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGAt                        >  2:96648/1‑139 (MQ=255)                                                                                    tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGAt                        >  2:265935/1‑139 (MQ=255)                                                                                    tACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGAt                        >  1:705206/1‑139 (MQ=255)                                                                                      cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATg                                                                                                   <  1:410720/62‑1 (MQ=255)                                                                                      cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATg                                                                                                   >  2:410720/1‑62 (MQ=255)                                                                                      cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                              >  2:1602949/1‑83 (MQ=255)                                                                                      cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                              <  1:1602949/83‑1 (MQ=255)                                                                                      cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATaa                      >  1:385146/1‑139 (MQ=255)                                                                                      cGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATaa                      >  2:240388/1‑139 (MQ=255)                                                                                                 aGGACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCcgcg           <  2:705206/139‑1 (MQ=255)                                                                                                   gACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                              >  1:651404/1‑70 (MQ=255)                                                                                                   gACTGTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCa                                                                              <  2:651404/70‑1 (MQ=255)                                                                                                       gTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATTAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGa                                           <  1:420136/101‑1 (MQ=255)                                                                                                       gTCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATTAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGa                                           >  2:420136/1‑101 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                               >  2:1766940/1‑64 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGcc                                                                               <  1:1766940/64‑1 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATc                                                                            <  1:10343/67‑1 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATc                                                                            >  2:10343/1‑67 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                   <  1:1767904/76‑1 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGatat                                                                   >  2:1767904/1‑76 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtctc                                                             >  2:484381/1‑82 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGtctc                                                             <  1:484381/82‑1 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGa    >  2:586844/1‑139 (MQ=255)                                                                                                        tCGGTTATTTAACTTTGTGGGGATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATACCATCTTTCGATATTGTCTCTGAAGTTGATCTTCATGAAGCACGTAACGCGGTCGATAACGCGAGCCGCCAAGTGGa    >  1:1441746/1‑139 (MQ=255)                                                                                                          ggTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGt  <  1:265935/139‑1 (MQ=255)                                                                                                          ggTTATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGt  <  1:96648/139‑1 (MQ=255)                                                                                                             tATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAgg                                                                                               >  1:1687440/1‑43 (MQ=255)                                                                                                             tATTTAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAgg                                                                                               <  2:1687440/43‑1 (MQ=255)                                                                                                                ttAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGc                                        >  1:1159351/1‑95 (MQ=255)                                                                                                                ttAACTTTGTGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGc                                        <  2:1159351/95‑1 (MQ=255)                                                                                                                         |                                                                                                                            CTCCTCACCCGCAACCACTCCGGGTGTTCAATAAGGCTATCCCTTAATTGTGCATGCTGTTGCGACTATGCACAATTAAGGGATACGTCCTGGTGCAGGACTGTCGGTTATTTAACTTTGCGGGTATTATGCTTCGCATCAAAAATGAAGGGAGAGGAAAAGATGCCATCTTTCGATATTGTCTCTGAAGTTGATCTTCAGGAAGCACGTAACGCGGTCGATAACGCGAGCCGCGAAGTGGAGT  >  NC_000913/444521‑444764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑