breseq  version 0.32.1  revision aa8f2595a244
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsT1000_846_TGTTAACT-ATTGACAT.R2.good.fq1,884,785198,492,563100.0%105.3 bases139 bases91.6%
errorsT1000_846_TGTTAACT-ATTGACAT.R1.good.fq1,884,785198,492,563100.0%105.3 bases139 bases91.6%
total3,769,570396,985,126100.0%105.3 bases139 bases91.6%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65277.04.0100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000005281
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000677
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.041

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.76259

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.8
R3.3.1

Execution Times

stepstartendelapsed
Read and reference sequence file input06:07:14 20 Jul 201806:07:53 20 Jul 201839 seconds
Read alignment to reference genome06:07:53 20 Jul 201806:09:54 20 Jul 20182 minutes 1 second
Preprocessing alignments for candidate junction identification06:09:54 20 Jul 201806:10:41 20 Jul 201847 seconds
Preliminary analysis of coverage distribution06:10:41 20 Jul 201806:11:58 20 Jul 20181 minute 17 seconds
Identifying junction candidates06:11:58 20 Jul 201806:12:04 20 Jul 20186 seconds
Re-alignment to junction candidates06:12:04 20 Jul 201806:12:30 20 Jul 201826 seconds
Resolving best read alignments06:12:30 20 Jul 201806:13:49 20 Jul 20181 minute 19 seconds
Creating BAM files06:13:49 20 Jul 201806:14:56 20 Jul 20181 minute 7 seconds
Tabulating error counts06:14:56 20 Jul 201806:15:26 20 Jul 201830 seconds
Re-calibrating base error rates06:15:26 20 Jul 201806:15:26 20 Jul 20180 seconds
Examining read alignment evidence06:15:26 20 Jul 201806:21:04 20 Jul 20185 minutes 38 seconds
Polymorphism statistics06:21:04 20 Jul 201806:21:04 20 Jul 20180 seconds
Output06:21:04 20 Jul 201806:22:11 20 Jul 20181 minute 7 seconds
Total 14 minutes 57 seconds