| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 932,340 | (A)8→7 | intergenic (‑290/‑255) | trxB ← / → lrp | thioredoxin reductase, FAD/NAD(P)‑binding/leucine‑responsive global transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 932,333 | 0 | A | . | 94.9% | 143.3 / 2.9 | 39 | intergenic (‑283/‑262) | trxB/lrp | thioredoxin reductase, FAD/NAD(P)‑binding/leucine‑responsive global transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base . (19/18); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.07e-01 | |||||||||||
TAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGAT > NC_000913/932201‑932434 | tAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa < 2:687079/139‑1 (MQ=255) aaTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca < 1:737681/139‑3 (MQ=255) ttCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTa > 1:826066/1‑139 (MQ=255) aaGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATgcg < 1:596067/139‑1 (MQ=255) gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTtat < 1:268617/129‑1 (MQ=255) gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTtat > 2:268617/1‑129 (MQ=255) gATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATaa > 1:600741/1‑139 (MQ=255) ccTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGtt > 1:893469/1‑139 (MQ=255) tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa < 2:893469/139‑1 (MQ=255) atatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca > 1:278356/1‑84 (MQ=255) atatCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca < 2:278356/86‑3 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGTAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa > 2:622697/1‑139 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca < 2:512223/83‑3 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca > 1:840250/1‑81 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca > 1:512223/1‑81 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca < 2:840250/83‑3 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaa > 1:1244893/1‑78 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaa < 2:1244893/78‑1 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa > 2:795704/1‑139 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa < 2:209309/139‑1 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGa > 2:1052166/1‑139 (MQ=255) aTGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCa < 2:38932/94‑1 (MQ=255) aTGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCa > 1:38932/1‑94 (MQ=255) ttgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGt < 2:600741/139‑1 (MQ=255) tgtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTa > 1:949851/1‑139 (MQ=255) gtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg > 1:586432/1‑121 (MQ=255) gtACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg < 2:586432/121‑1 (MQ=255) tACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTAtt < 1:823713/76‑1 (MQ=255) tACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTAtt > 2:823713/1‑76 (MQ=255) tACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTAt < 1:804179/75‑1 (MQ=255) tACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTAt > 2:804179/1‑75 (MQ=255) aCTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATgg < 1:1052166/139‑1 (MQ=255) cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatgc < 2:666145/95‑1 (MQ=255) cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatgc > 1:666145/1‑95 (MQ=255) cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatg > 2:64318/1‑94 (MQ=255) cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatg < 1:64318/94‑1 (MQ=255) aaaaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATaa < 2:1027916/86‑1 (MQ=255) aaaaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATaa > 1:1027916/1‑86 (MQ=255) aaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaacagt < 1:854413/66‑5 (MQ=255) aaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaacagt > 2:854413/1‑62 (MQ=255) tGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTatat > 2:1182226/1‑70 (MQ=255) tGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTatat < 1:1182226/70‑1 (MQ=255) tGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTg < 1:978551/138‑1 (MQ=255) tGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTg > 2:978551/1‑138 (MQ=255) taCAATCCCCTGGTGTTTTGCTAAAACATTCGAGGAAG‑AAAAAAACGGTATGCTTATATGCGCATAACCATGCATGTAAATACCATGTATACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGCGAt < 2:724235/138‑1 (MQ=255) ggTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca > 2:618936/1‑34 (MQ=255) ggTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca < 1:618936/36‑3 (MQ=255) ttttGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTatat < 2:1087900/44‑1 (MQ=38) ttttGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTatat > 1:1087900/1‑44 (MQ=38) ttGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTtat > 2:1290230/1‑41 (MQ=255) ttGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTtat < 1:1290230/41‑1 (MQ=255) ttGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGc > 2:477802/1‑100 (MQ=255) ttGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGc < 1:477802/100‑1 (MQ=255) | TAAATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGAT > NC_000913/932201‑932434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |