Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,168,350 Δ1 bp coding (483/633 nt) ycfQ ← repressor for bhsA(ycfR)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,168,3480G.100.0% 146.1 / NA 35coding (485/633 nt)ycfQrepressor for bhsA(ycfR)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/17);  total (18/17)

TTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATC  >  NC_000913/1168212‑1168476
                                                                                                                                        |                                                                                                                                
ttCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCgga                                                                                                                                >  2:688136/1‑138 (MQ=255)
     gCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTcc                                                                                                                          >  1:1138085/1‑139 (MQ=255)
       cATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTcccc                                                                                                                        <  2:1138085/139‑1 (MQ=255)
                               aTCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATaaaaa                                                                                                <  1:688136/139‑1 (MQ=255)
                                                                    cgcTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGAt                                                           >  1:57404/1‑139 (MQ=255)
                                                                     gcTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGAtt                                                          >  1:1049885/1‑139 (MQ=255)
                                                                         aTCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCaa                                                      >  2:15042/1‑139 (MQ=255)
                                                                                   ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCa                                                                         <  2:873756/110‑1 (MQ=255)
                                                                                   ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCa                                                                         >  1:873756/1‑110 (MQ=255)
                                                                                   ccTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCg                                            >  1:650861/1‑139 (MQ=255)
                                                                                        ataataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGata                                         >  2:1135459/1‑137 (MQ=255)
                                                                                        ataataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGata                                         <  1:1135459/137‑1 (MQ=255)
                                                                                           ataCAATTAAGGTATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTcc                                    >  2:243079/1‑139 (MQ=255)
                                                                                           ataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATaaa                                                                                                  <  1:20150/77‑1 (MQ=255)
                                                                                           ataCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATaaa                                                                                                  >  2:20150/1‑77 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCa                                                       >  1:499260/1‑119 (MQ=255)
                                                                                            taCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCa                                                       <  2:499260/119‑1 (MQ=255)
                                                                                             aCAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTg                                  <  1:1184691/139‑1 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCggat                                                                                                                               <  2:1039602/43‑3 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCggat                                                                                                                               >  1:1039602/1‑41 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGc                                             <  1:335843/124‑1 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGc                                             >  2:335843/1‑124 (MQ=255)
                                                                                                 ttAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATc                              >  2:1295365/1‑139 (MQ=255)
                                                                                                    aGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTgg                           >  1:678977/1‑139 (MQ=255)
                                                                                                      gAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCcgcg                                                                                                                     <  2:153420/47‑1 (MQ=255)
                                                                                                      gAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCcgcg                                                                                                                     >  1:153420/1‑47 (MQ=255)
                                                                                                      gAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTgggg                         >  1:1016586/1‑139 (MQ=255)
                                                                                                         ttCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAAc                                                     >  1:36255/1‑108 (MQ=255)
                                                                                                         ttCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAAc                                                     <  2:36255/108‑1 (MQ=255)
                                                                                                         ttCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGata                                         <  1:683946/120‑1 (MQ=255)
                                                                                                         ttCTGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGata                                         >  2:683946/1‑120 (MQ=255)
                                                                                                            tGCCAGATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAggg                   <  1:15042/139‑1 (MQ=255)
                                                                                                                 gATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGTTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg              <  2:1178132/139‑1 (MQ=255)
                                                                                                                 gATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg              <  2:655425/139‑1 (MQ=255)
                                                                                                                 gATGTGTCACGTCACAGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg              <  2:1049885/139‑1 (MQ=255)
                                                                                                                             cacaGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATc  <  2:678977/139‑1 (MQ=255)
                                                                                                                              acaGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg              >  2:123489/1‑126 (MQ=255)
                                                                                                                              acaGTGGGGCGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGgtggtg              <  1:123489/126‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                
TTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATCAGTTTTTCCAGCGATGCACCTTCGCGTGCGCTGATCGACATCCCCTGAATAATACAATTAAGGAATTCTGCCAGATGTGTCACGTCACAGTGGGGCGGGATTTCCCCGCGCGCTTGTCGTTGACATAAAAACTGCTGCAAAGTGCGCTCTTGCATCGCATGGCGTGATTTCAACGTATTGGCGATATCTCCTGAATCTGGGGAGAGGGTGGTGCAGTTGTTGATC  >  NC_000913/1168212‑1168476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: